Morphological and molecular identification of Explanatum explanatum in domestic water buffalo in Pakistan

Chaudhry, Umer; Paridon, Bradley van; Lejeune, Manigandan; Shabbir, Muhammad Zubair; Rashid, Imran; Ashraf, Kamran; Ashraf, Shoaib; Gilleard, John S.; Sargison, Neil

doi:10.1016/j.vprsr.2017.02.002

Cited by 11 publications

(14 citation statements)

References 35 publications

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“…The results of our study showed that five paramphistomes isolates showed no variation in ITS-2, while the result of the phylogenetic analysis showed that there was a close genetic relationship between the sample and C. fabaceus that was a member of the Cladorchiidae family, similar to the sample’s family (Figure-2). It has been reported before that ITS-2 might be a useful marker in recovering their phylogenetic species and in identifying paramphistomoid species in every life stage [9-16]. Therefore, this nucleotide sequence data may reveal the life cycle, pathogenicity, and also epidemiological studies of this Sumatran elephant paramphistomoid.…”

Section: Discussionmentioning

confidence: 96%

“…Therefore, an alternative approach to identifying these species is necessary. Polymerase chain reaction (PCR)-based techniques using the ribosomal deoxyribonucleic acid (rDNA) second internal transcribed spacer (ITS-2) have proven that the rDNA ITS-2 was a useful marker for identifying the paramphistomoid species in every life stage and also in recovering their phylogenetic species [9-16].…”

Section: Introductionmentioning

confidence: 99%

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Morphological and molecular identification of Pfenderius heterocaeca (Trematode: Paramphistomoidea) from Sumatran elephant (Elephas maximus sumatranus)

Firdausy¹,

Prahardani²,

Wusahaningtyas³

et al. 2019

Vet World

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Background and Aim: Paramphistomiasis is common in tropical countries such as Indonesia and affects livestock and various endemic wild animals such as Sumatran elephants. However, the specific species of paramphistomoid worm that causes paramphistomiasis are rarely reported. The study aims at identifying paramphistomoid worm that infects Sumatran elephants. Materials and Methods: Flukes were collected from the feces of five semi-captive Sumatran elephants that lived at Tegal Yoso Elephant Response Unit in Way Kambas National Park, in 2018, after treatment of oxyclozanide 1 g at the dose of approximately 5-8 mg/kg of body weight. Eight paramphistomoid worms were flattened and stained in Semichon's carmine for morphological identification, and five other worms were used for molecular identification at second internal transcribed spacer (ITS-2) of ribosomal deoxyribonucleic acid sequence. Results: Forty-five flukes were collected from five Sumatran elephants in Lampung, Indonesia. Eight paramphistomoid worms were morphologically identified as Pfenderius heterocaeca> and five isolates did not show any variation in ITS-2. Phylogenetic analysis showed that there was a close genetic relationship between our sample and Chiorchis fabaceus that had a family similar to the samples. Conclusion: Based on the morphological and molecular characteristics, the paramphistomoids found in Sumatran elephant on Way Kambas National Park are P. heterocaeca. Keywords: internal transcribed spacer-2, paramphistomiasis, Pfenderius spp., Sumatran elephant.

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Morphological and molecular identification of Pfenderius heterocaeca (Trematode: Paramphistomoidea) from Sumatran elephant (Elephas maximus sumatranus)

Firdausy¹,

Prahardani²,

Wusahaningtyas³

et al. 2019

Vet World

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“…A study from Pakistan [11] identified the ITS2 region of G. explanatum as well from Rawalpindi, Punjab province.…”

Section: Discussionmentioning

confidence: 99%

“…from Paramphistomum leydeni at 7 nucleotide sites [8,11] . The current sequence ITS2 of 286 bp was genetically identical to G. explanatum of Myanmar (AB743577) and a difference of single nucleotide with Bangladesh (LC101683), and single nucleotide difference reported with Myanmar [11] . Interspecific variations were one to two nucleotides with India (KF564869) and China (KT198990) respectively.…”

Section: Discussionmentioning

confidence: 99%

“…Although, considerable attention has been paid to adverse pathology and epidemiology of G. explanatum in Pakistan, relatively little attention has been paid to the systematics of the species. So far, a single report on molecular characterization of G. explanatum comes out from Punjab [11] . Thus, the aim of this study was to determine the genetic structure of G. explanatum in Pakistan by using the complete ribosomal DNA (ITS1-5.8S-ITS2) regions.…”

Section: Genetic Studies On Gigantocotyle Explanatummentioning

confidence: 99%

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Kuzeybatı Pakistan’daki Buffalo’lardan Gigantocotyle explanatum’un Genetik Karakterizasyonu

Ullah¹,

Afshan²,

Arshad³

et al. 2019

Kafkas Univ Vet Fak Derg

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Morphological and molecular identification of lymnaeid snail and trematodes cercariae in different water bodies in Perak, Malaysia

et al. 2023

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Lymnaeid snails are vital in transmitting trematode cercariae as an intermediate host that can infect buffalo and other ruminants and humans, causing signi cant economic losses. The study aimed to conduct morphological and molecular identi cation of snails and cercariae collected from the selected buffalo farms under palm oil integration in Perak, Malaysia. A cross-sectional study was conducted, and 35 water were investigated for the presence or absence of snails. A total of 836 lymnaeid snails were collected from three marshes wetlands. To identify the snail family and species, morphological identi cation was performed on each snail's shell, and to identify trematode cercariae types; the crushing method was used to observe the cercarial stage inside each snail's body. In addition, the Internal Transcribed Spacer 2 (ITS2) region and Cytochrome c oxidase subunit 1 (cox1) was used as the target gene to identify the snail species and cercarial types up to the species level. The result showed that the collected snails belong to the family Lymnaeidae and Radix rubiginosa species. The infection rate by cercarial emergence in snails was 8.73%. Five morphological cercarial types were observed which include: echinostome, xiphidiocercariae, gymnocephalous, brevifurcate-apharyngeate distome, and vivax. Using molecular methods, the identi ed cercariae belong to the three families, including Echinostomatidae, Plagiorchiidae, and Fasciolidae. This is the rst report on R. rubiginosa and different types of trematodes cercariae in buffalo farms under palm oil integration in Perak. Our nding con rmed that R. rubiginosa could serve as an intermediate host for a range of parasitic trematodes in Perak.

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Morphological and molecular identification of Explanatum explanatum in domestic water buffalo in Pakistan

Abstract: Short Communication Morphological and molecular identification of Explanatum explanatum in domestic water buffalo in Pakistan

Cited by 11 publications

References 35 publications

Morphological and molecular identification of Pfenderius heterocaeca (Trematode: Paramphistomoidea) from Sumatran elephant (Elephas maximus sumatranus)

Morphological and molecular identification of Pfenderius heterocaeca (Trematode: Paramphistomoidea) from Sumatran elephant (Elephas maximus sumatranus)

Kuzeybatı Pakistan’daki Buffalo’lardan Gigantocotyle explanatum’un Genetik Karakterizasyonu

Morphological and molecular identification of lymnaeid snail and trematodes cercariae in different water bodies in Perak, Malaysia

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