Morphological and Molecular Identification of the Neoscytalidium dimidiatum, and Evaluation of Kaolin and Magnesium Oxide Nano-particles Efficacy to Control in Vitro

Abstract

The study was conducted during season of 2018-2019 in the College of Agricultural Engineering Sciences / University of Baghdad.The aim of examining and diagnosing the pathogenic fungus Neoscytalidium dimidiatum from apple, mulberry and castor plants. The results of the electrophoresis of DNA extracted from the pathogen on agarose gel showed the presence of one band with a molecular weight of 550bp, The nucleotide sequences of the fungus have been deposited in Genebank under accession number MW011739.1, MW011738.1, MW011740.1 respectively.The results showed that the magnesium oxide nanoparticles and the score pesticide achieved a high antagonistic capacity reaching 100%,while the effect of kaolin in its three concentrations as the concentration of 0.5% did not achieve any inhibition in the growth of the fungal hyphae,while the average percentage of inhibition reached 37.08% at the concentration of 2% and 35.69 at the concentration of 1%.