Morphological and molecular identification of java barb (Barbonymus gonionotus) from East Java Province

Abstract

This study aims is compare the identification of Java barb fish (Barbonymus gonionotus) in East Java Province by morphometric and molecular identification. Molecular identification was conducted using mitochondrial DNA based on the Cytochrome Oxidase Sub Unit I (COI) region. Java barb fish samples were collected from various regions in East Java Province including Gresik, Mojokerto, Madiun, Malang, and Banyuwangi. The identification results by morphological and molecular methods showed the same results, namely Barbonymus gonionotus. Based on the morphological identification, Barbonymus gonionotus has a long and flat body shape with a raised back, terminal mouth position, silver body colour with a darker dorsal area, dorsal fin shape with weakly hardened rays, and homocercal caudal fin shape. The morphometric measurements of Java barb fish using the one-way ANOVA test showed that the five locations differed significantly. The results of the molecular research showed that all java barb fish sequences were successfully amplified with the COI gene (648 bp). The BLASTN results show the percentage identity ranges from 99.85-100%.