Morphological and Molecular Identification Aphids of Rosae

Jalalizand, Alireza; Mirhendi, H; Karimi, Azadeh; Modaresi, Mehrdad; Mahmoodi, Esmaeil

doi:10.1016/j.apcbee.2012.11.003

Cited by 12 publications

(8 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In overall, aphid species identification in the earlier study was done grounded on morphological and coloration methods (Jalalizand et al, 2012), while in this paper focused on mitochondrion gene via molecular technique which were commonly used for identification species, that not used previously in this region, so that the obtained results were a vital and established a very effective determination in almost all used species by COI mt DNA. Whereas this study concentrated on the mitochondrion gene using a molecular method that was frequently used to identify species "that had not been utilized before in this region".…”

Section: Resultsmentioning

confidence: 99%

“…In several cases, identification of species based on coloration of pattern is challenging. Because Aphid morphology is influenced by a variety of environmental and physiological factors (Jalalizand et al,2012). In cases when morphological and ecological evidence remain unclear, molecular methods are indeed very helpful (Choe et al 2006 andValenzuela et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Since a proper detection of insects is really the first stage in pest management. Precise identification of aphid species is required such as using of molecular techniques (Jalalizand et al,2012). In an effort to identify different aphid species and get over the constraints of morphological identifications, several molecular approaches have been investigated.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular Identification of Some Aphid Species (Homoptera; Aphididae) Based on RFLP-PCR Technique

Muhammad¹,

Mawlud²,

Taha³

et al. 2022

Mesopotamia Journal of Agriculture

View full text Add to dashboard Cite

This work includes, identification eight species of Aphids (Homoptera: Aphididae) which collected from the leaves of different plants in many localities of Erbil governorate Kurdistan region-Iraq from the period May till July 2022, these are: Chaitophorus salijaponicus, Aphis fabae, Macrosiphum rosae, Capitophorus carduinus, Myzus persicae, Aphis ruborum, Aphis punca, and Aphis gossypii. The mitochondrial cytochrome C Oxidase subunit I (COI) gene used for identification these species. DNA was isolated, and a band of 550 bp of mt COI gene was amplified during the PCR amplification. The amplicons were digested with HinfI and DdeI restriction enzymes. The restricted fragments produced by RFLP technique were proved by agarose gel electrophoresis. The results illustrated that digested amplicons were given bands according to their cut sites. This study presented that studying aphids to detect their species through a RFLP-PCR technique by using these restriction enzymes can distinguish some species with reliable results. HinfI and DdeI REs could not distinguish all species, HinfI only discriminated species Macrosiphum rosae, Capitophorus carduinus, Myzus persicae and Aphis gossypii, but DdeI identified the remain species, Chaitophorus salijaponicus, Macrosiphum rosae, Myzus persicae and Aphis ruborum, within and among other species exactly. The study suggested using other restriction enzymes to provide full recognition profile for all species.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Identification of Some Aphid Species (Homoptera; Aphididae) Based on RFLP-PCR Technique

Muhammad¹,

Mawlud²,

Taha³

et al. 2022

Mesopotamia Journal of Agriculture

View full text Add to dashboard Cite

show abstract

“…A precise taxonomic identification of aphids, especially in the field, is challenging because of morphotypes with discreet morphometric differences within the same species [5] and chromatic variations unleashed by environmental stimuli [6] and intrinsic factors such as overpopulation or the type of host [7]. Molecular identification approaches, overcome the difficulties posed by morphological traits in telling aphid species apart.…”

Section: Introductionmentioning

confidence: 99%

Identification and life history of aphids associated with chili pepper crops in southwestern Colombia

et al. 2020

View full text Add to dashboard Cite

Viral diseases, transmitted by aphids, are the most limiting problems in chili pepper crops. Understanding the demographic features of these aphids, may thus assist the design of better disease control strategies in chili peppers. Aphid species found in chili pepper crops in south western Colombia were identiﬁed as Aphis gossypii Glover and Myzus persicae (Sulzer). An array of life-history parameters of both aphid species were investigated at (25 ± 0.5) ◦C, (75 ± 1.75) % r.h., L12:D12, and LS 5-Light Storm in chili pepper crops. Both aphid populations consisted only of parthenogenetic females, showing a similar average development time—from the ﬁrst nymphal instar to the post-reproductive adult—, female longevity, and daily average fertility values. The length of the reproductive period was higher for M. persicae. A. gossypii reached its adult state signiﬁcantly faster than M. persicae. The intrinsic rate of population growth (rm) was lower for M.persicae (0.39) compared to A. gossypii (0.43). Results showed a potential for fast population growth in both species, which would enhance their role as virus vectors. The information acquired is essential to develop pest management initiatives for these two aphid species.

show abstract

“…1). This species cause direct damage by sap sucking from young leaves and developing flower buds which in turn results in discoloration of leaves, stunted growth and gall formation, while as indirect loss is incurred by honeydew secretion on flowers and surface of leaves on which molds grow resulting in reduction in photosynthesis and finally the yield (Jalalizand, 2012). All these factors together cause significant damage to rose plants by decreasing their beauty and the value of cut flowers.…”

Section: Introductionmentioning

confidence: 99%

Seasonal incidence and bionomics of rose aphid, Macrosiphum rosae (Linnaeus, 1758), (Hemiptera: Aphididae) in Kashmir, India

Yousuf

Buhroo

2020

AAS

View full text Add to dashboard Cite

<p>Rose is the principal flower of the world floriculture industry that is being exclusively used as cut flower, potted plant and garden plant. It plays significant part in numerous industries viz. food, perfumery and cosmetic industries. About 96 % of women’s perfumes contain true Bulgarian rose oil. Roses are well acclimatized in Jammu & Kashmir because of its suitable agro climatic conditions which can permit its large scale production and rose products produced in the state are at par with the international standards. But the aesthetic and commercial value of roses is greatly lowered by numerous insect pests resulting in low yield. However, its major pest include aphid species most notoriously Macrosiphum rosae that pose many challenges and threats to rose plant cultivation. Aphid colonies on roses result in reduction of medical value of the plant and cause economic losses to growers particularly during spring and summer season. In order to reduce the economic losses inflicted by rose aphid, it is necessary to study different biological parameters of this pest species so that an effective management plan can be formulated.</p>

show abstract

Morphological and Molecular Identification Aphids of Rosae

Cited by 12 publications

References 2 publications

Molecular Identification of Some Aphid Species (Homoptera; Aphididae) Based on RFLP-PCR Technique

Molecular Identification of Some Aphid Species (Homoptera; Aphididae) Based on RFLP-PCR Technique

Identification and life history of aphids associated with chili pepper crops in southwestern Colombia

Seasonal incidence and bionomics of rose aphid, Macrosiphum rosae (Linnaeus, 1758), (Hemiptera: Aphididae) in Kashmir, India

Contact Info

Product

Resources

About