Morphological and molecular-genetic distinctions between adult mosquitoes Culex torrentium Martini and C. pipiens Linnaeus (Diptera, Culicidae) from Moscow Province

Fedorova, M. V.; Шайкевич, Е. В.

doi:10.1134/s0013873807020017

Cited by 22 publications

(20 citation statements)

References 13 publications

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“…The 3' end of the ITS2 region in C. torrentium contains dinucleotide repeats in various combinations (Fedorova, Shaikevich, 2007). Variability of the ITS2 region in the studied specimens of C. torrentium is 2 %, and the difference between C. p. pipiens and C. torrentium reaches 12.5 % (Fig.…”

Section: Comp Cytogenet 2010 4(2)mentioning

confidence: 99%

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Polymorphism of mitochondrial COI and nuclear ribosomal ITS2 in the Culex pipiens complex and in Culex torrentium (Diptera: Culicidae)

Шайкевич¹,

Захаров²

2010

CCG

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Abstract. Polymorphism of the mtDNA gene COI encoding cytochrome C oxidase subunit I was studied in the mosquitoes Culex pipiens Linnaeus, 1758 and C. torrentium Martini, 1925 from sixteen locations in Russia and in three laboratory strains of subtropical subspecies of the C. pipiens complex. Representatives of this complex are characterized by a high ecological plasticity and there are signifi cant ecophysiological differences between its morphologically similar members. The full-size DNA sequence of the gene COI spans 1548 bp and has a total A+T content of 70.2 %. The TAA is a terminating codon in all studied representatives of the C. pipiens complex and C. torrentium. 64 variable nucleotide sites (4 %) were found, fi fteen haplotypes were detected, and two heteroplasmic specimens of C. torrentium were recorded. COI haplotype diversity was low in Wolbachia-infected populations of the C. pipiens complex. Monomorphic haplotypes were found in C. p. quinquefasciatus and C. p. pipiens f. molestus. Three haplotypes were detected for the C. p. pipiens, but these haplotypes were not population-specifi c. On the other hand, each of the ten studied Wolbachia-uninfected C. torrentium individuals from three different populations had unique mitochondrial haplotypes. Polymorphism of the 478-bp ITS2 nucleotide sequences was similar in infected C. p. pipiens and C. p. pipiens f. molestus and uninfected C. torrentium specimens. The ITS2 genetic distance between C. p. pipiens and C. torrentium reached 12.5 %. Possible effects of Wolbachia invasion on C. pipiens populations are discussed.

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Section: Comp Cytogenet 2010 4(2)mentioning

confidence: 99%

“…Similarities are often results in misidentifi cation of these two species. However, genetic differences allow precise distinction of these species using the appropriate DNA markers (Fedorova, Shaikevich, 2007).…”

Section: Introductionmentioning

confidence: 99%

Polymorphism of mitochondrial COI and nuclear ribosomal ITS2 in the Culex pipiens complex and in Culex torrentium (Diptera: Culicidae)

Шайкевич¹,

Захаров²

2010

CCG

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“…Unfortunately, morphological features are often insufficient for identifying species of bloodsucking mosquitoes as some are very variable and consequently unreliable [for example, siphonal index of C. pipiens larvae (Fedorova & Shaikevich, 2007)], while many diagnostic features (especially paleolae and chaetae) are not present on collected material. This problem can be solved by using molecular-genetic analysis and developing DNA markers for specifically identifying species and subspecies of these insects.…”

Section: Introductionmentioning

confidence: 99%

Molecular approach for identification of mosquito species (Diptera: Culicidae) in Province of Alessandria, Piedmont, Italy

Talbalaghi¹,

Шайкевич²

2011

Eur. J. Entomol.

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Abstract. The aim of the present work was to identify cryptic species in the Anopheles maculipennis and Culex pipiens complexes and to study the genetic structure of the dominant mosquito species Ochlerotatus caspius (Diptera: Culicidae) in the Province of Alessandria close to the vast area untreated rice fields in Piedmont, NW Italy. With the help of PCR-RFLP analysis, four members of the Anopheles maculipennis complex were identified: A. messeae, A. maculipennis, A. sacharovi and A. atroparvus. Only C. pipiens f. molestus was identified in 11 habitats studied in Piedmont. Partial sequences of the cytochrome c oxidase subunit 1 (COI) mitochondrial gene and the second internal transcribed spacer (ITS2) of nuclear ribosomal RNA genes for Italian O. caspius are reported here for the first time. The results indicate that this species diverged from Iranian representatives of this species about one million years ago. The great diversity of mosquito species in Piedmont considerably increases the risk of vector-borne dise ases.

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“…References for primers: 1 Simon et al (1994); 2 Lunt et al (1996); 3 Shaikevich (2007); 4 Folmer et al (1994). References for studied regions: a Shaikevich et al (2005), Fedorova & Shaikevich (2007), Vinogradova et al (2007); b Cywinska et al (2006), Shaikevich (2007), Vinogradova et al (2007).…”

Section: Methodsmentioning

confidence: 99%

“…torrentium (Shaikevich et al , 2005; Vinogradova & Shaikevich, 2007; Vinogradova et al , 2007). Polymerase chain reaction (PCR) amplifications of the nuclear ace ‐2 gene (Smith & Fonesca, 2004) and the ribosomal internal transcribed spacer 2 (ITS2) gene (Fedorova & Shaikevich, 2007; Vinogradova & Shaikevich, 2007) have been used to detect polymorphism between the species. Genetic polymorphism has also been detected by using restriction enzymes to digest varying parts of the mitochondrial cytochrome c oxidase subunit I (COI) gene (Shaikevich et al , 2005; Fedorova & Shaikevich, 2007; Shaikevich, 2007; Vinogradova & Shaikevich, 2007; Vinogradova et al , 2007).…”

Section: Introductionmentioning

confidence: 99%

A sensitive and reliable restriction enzyme assay to distinguish between the mosquitoes Culex torrentium and Culex pipiens

Hesson

Lundström

Halvarsson

et al. 2010

Medical and Veterinary Entomology

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Culex pipiens pipiens Linnaeus and Culex torrentium Martini (Diptera: Culicidae) are closely related vector species that exist sympatrically in Europe. The two species are morphologically almost identical and can only be distinguished with certainty by characters of the male genitalia. Hence, correct species identification and conclusions on distribution and vector status are very difficult and often neglected. Therefore, we developed a reliable and simple mitochondrial cytochrome c oxidase subunit I (COI) gene restriction enzyme assay to discriminate between Cx. pipiens and Cx. torrentium, based on the analysis of morphologically identified male specimens. We sequenced approximately 830 bp in the 3' region of the mitochondrial COI gene of 18 morphologically identified males of Cx. pipiens and Cx. torrentium. Two restriction enzymes (FspBI and SspI) that could distinguish between the two species according to species-specific differences in these sequences were chosen. The restriction enzymes were tested on 227 samples from Sweden and verified by sequencing 44 of them. The enzyme FspBI correctly identified all investigated samples; the enzyme SspI identified all samples except one Cx. torrentium. We hope the method and the findings presented here will help to shed light on the true distribution and relative proportions of the two species in Europe.

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Morphological and molecular-genetic distinctions between adult mosquitoes Culex torrentium Martini and C. pipiens Linnaeus (Diptera, Culicidae) from Moscow Province

Cited by 22 publications

References 13 publications

Polymorphism of mitochondrial COI and nuclear ribosomal ITS2 in the Culex pipiens complex and in Culex torrentium (Diptera: Culicidae)

Polymorphism of mitochondrial COI and nuclear ribosomal ITS2 in the Culex pipiens complex and in Culex torrentium (Diptera: Culicidae)

Molecular approach for identification of mosquito species (Diptera: Culicidae) in Province of Alessandria, Piedmont, Italy

A sensitive and reliable restriction enzyme assay to distinguish between the mosquitoes Culex torrentium and Culex pipiens

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