Morphological and molecular genetic characterization of Cryptoperidiniopsis brodyi (Dinophyceae) from Australia-wide isolates

Park, Tae-Gyu; Bolch, Christopher J. S.; Hallegraeff, Gustaaf M.

doi:10.1016/j.hal.2007.02.004

Cited by 8 publications

(9 citation statements)

References 53 publications

(69 reference statements)

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“…The present study confirmed previous findings of the conserved nature of the rDNA regions in P. piscicida (Tengs et al. 2003, Park et al. 2007a,b) and also indicated sequence conservation of the COI gene; however, a polymorphic site in the ITS1 region was identified and confirmed by RFLP analysis.…”

Section: Discussionsupporting

confidence: 92%

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GENETIC VARIATION AMONG STRAINS OF PSEUDOPFIESTERIA SHUMWAYAE AND PFIESTERIA PISCICIDA (DINOPHYCEAE)¹

Small

Shields

Haas

et al. 2009

Journal of Phycology

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The putatively toxic dinoflagellates Pseudopfiesteria shumwayae (Glasgow et J. M. Burkh.) Litaker, Steid., P. L. Mason, Shields et P. A. Tester and Pfiesteria piscicida Steid. et J. M. Burkh. have been implicated in massive fish kills and of having negative impacts on human health along the mid-Atlantic seaboard of the USA. Considerable debate still remains as to the mechanisms responsible for fish mortality (toxicity vs. micropredation) caused by these dinoflagellates. Genetic differences among these cultures have not been adequately investigated and may account for or correlate with phenotypic variability among strains within each species. Genetic variation among strains of Ps. shumwayae and P. piscicida was examined by PCR-RFLP analysis using cultures obtained from the Provasoli-Guillard National Center for Culture of Marine Phytoplankton (CCMP), as well as those from our own and other colleagues' collection efforts. Examination of restriction digest banding profiles for 22 strains of Ps. shumwayae revealed the presence of 10 polymorphic restriction endonuclease sites within the first and second internal transcribed spacers (ITS1 and ITS2) and the 5.8S gene of the rDNA complex, and the cytochrome oxidase subunit I (COI) gene. Three compound genotypes were represented within the 22 Ps. shumwayae strains. Conversely, PCR-RFLP examination of 14 strains of P. piscicida at the same ITS1, 5.8S, and ITS2 regions revealed only one variable restriction endonuclease site, located in the ITS1 region. In addition, a dinoflagellate culture listed as P. piscicida (CCMP 1928) and analyzed as part of this study was identified as closely related to Luciella masanensis P. L. Mason, H. J. Jeong, Litaker, Reece et Steid.

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Section: Discussionsupporting

confidence: 92%

“…shumwayae and P. piscicida by ballast water, as has been suggested by Doblin et al. (2004) and Park et al. (2007a).…”

Section: Discussionsupporting

confidence: 69%

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GENETIC VARIATION AMONG STRAINS OF PSEUDOPFIESTERIA SHUMWAYAE AND PFIESTERIA PISCICIDA (DINOPHYCEAE)¹

Small

Shields

Haas

et al. 2009

Journal of Phycology

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“…The transitional nature of this sample was also reflected in the rest of the OTUs, which were affiliated to the aerobic genera Maribacter (Nedashkovskaya et al 2004), Mesonia (Nedashkovskaya et al 2006) and Sulfitobacter (Sorokin et al 1995). Maribacter is a halotolerant taxon (Nedahskovskaya et al 2004) and it has been reported to occur in evaporitic habitats (Dorador et al 2009) while members of the Sulfitobacter genus can grow at 10% NaCl salinity (Park et al 2007), suggesting these OTUs could represent metabolically active bacteria in these samples.…”

Section: Methodsmentioning

confidence: 99%

Inter-comparison of the potentially active prokaryotic communities in the halocline sediments of Mediterranean deep-sea hypersaline basins

et al. 2015

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The sediment microbiota of the Mediterranean deep--sea anoxic hypersaline basins (DHABs) are understudied relative to communities in the brines and halocline waters. In this study, the active fraction of the prokaryotic community in the halocline sediments of L' Atalante, Urania and Discovery DHABs was investigated based on extracted total RNA and 454 pyrosequencing of the 16S rRNA gene. Bacterial and archaeal communities were different in the sediments underlying the halocline waters of the three habitats, reflecting the unique chemical settings of each basin. The relative abundance of unique operational taxonomic units (OTUs) was also different between deep--sea control sediments and sediments underlying DHAB haloclines, suggesting adaptation to the steep DHAB chemical gradients. Only a few OTUs were affiliated to known bacterial halophilic and/or aerobic groups. Many OTUs, including some of the dominant ones, were related to aerobic taxa. Archaea were detected only in few halocline samples, with lower OTU richness relative to Bacteria, and were dominated by taxa associated with methane cycling. This study suggests that while metabolically active prokaryotic communities appear to be present in sediments underlying the three DHABs investigated, their diversity and activity are likely to be more reduced in sediments underlying the brines.3

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“…Cryptoperidiniopsis brodyi appears to be widely distributed in Australia and P. piscicida was also found in ballast water from Indonesia (Park et al, 2007a). The objective of this study was to examine capability of P. piscicida from Indonesia and C. brodyi from Australia to cause Pacific oyster Crassostrea gigas (Thunberg, 1793) and brine shrimp Artemia salina (Linnaeus, 1758) deaths.…”

Section: Introductionmentioning

confidence: 99%

Larval Crassostrea bivalve and Artemia brine shrimp bioassays to assess toxicity and micropredation by the heterotrophic dinoflagellates Cryptoperidiniopsis brodyi and Pfiesteria piscicida from Australian waters

Park¹,

Bolch²,

Hallegraeff³

2007

Journal of Plankton Research

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Morphological and molecular genetic characterization of Cryptoperidiniopsis brodyi (Dinophyceae) from Australia-wide isolates

Cited by 8 publications

References 53 publications

GENETIC VARIATION AMONG STRAINS OF PSEUDOPFIESTERIA SHUMWAYAE AND PFIESTERIA PISCICIDA (DINOPHYCEAE)¹

GENETIC VARIATION AMONG STRAINS OF PSEUDOPFIESTERIA SHUMWAYAE AND PFIESTERIA PISCICIDA (DINOPHYCEAE)¹

Inter-comparison of the potentially active prokaryotic communities in the halocline sediments of Mediterranean deep-sea hypersaline basins

Larval Crassostrea bivalve and Artemia brine shrimp bioassays to assess toxicity and micropredation by the heterotrophic dinoflagellates Cryptoperidiniopsis brodyi and Pfiesteria piscicida from Australian waters

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Morphological and molecular genetic characterization of Cryptoperidiniopsis brodyi (Dinophyceae) from Australia-wide isolates

Cited by 8 publications

References 53 publications

GENETIC VARIATION AMONG STRAINS OF PSEUDOPFIESTERIA SHUMWAYAE AND PFIESTERIA PISCICIDA (DINOPHYCEAE)1

GENETIC VARIATION AMONG STRAINS OF PSEUDOPFIESTERIA SHUMWAYAE AND PFIESTERIA PISCICIDA (DINOPHYCEAE)1

Inter-comparison of the potentially active prokaryotic communities in the halocline sediments of Mediterranean deep-sea hypersaline basins

Larval Crassostrea bivalve and Artemia brine shrimp bioassays to assess toxicity and micropredation by the heterotrophic dinoflagellates Cryptoperidiniopsis brodyi and Pfiesteria piscicida from Australian waters

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GENETIC VARIATION AMONG STRAINS OF PSEUDOPFIESTERIA SHUMWAYAE AND PFIESTERIA PISCICIDA (DINOPHYCEAE)¹

GENETIC VARIATION AMONG STRAINS OF PSEUDOPFIESTERIA SHUMWAYAE AND PFIESTERIA PISCICIDA (DINOPHYCEAE)¹