Morphological and molecular evidence support description of two new diatom species from the genus Ulnaria in Lake Baikal

Kulikovskiy, Maxim; Lange‐Bertalot, Horst; Annenkova, Nataliia V.; Gusev, Evgeniy; Kociolek, John Patrick

doi:10.5507/fot.2015.011

Cited by 27 publications

(19 citation statements)

References 34 publications

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“…They are a predominant component of lake sediments and are known to be suitable bioindicator proxies used in spatial and temporal lake environmental reconstructions [5][6][7]. Moreover, studies on large old lake systems have uncovered several endemic diatom taxa [8][9][10][11], which make them suitable organisms to investigate their community patterns in the light of taxa differentiation and formation of biodiversity. It is known that differences in diatom communities in various intra-lake settings have been attributed to strong variations in lake depth, light exposure, ice cover dynamics, habitat variability and lake chemistry including pH, salinity, phosphorous and nitrogen concentrations, as well as to catchment-related differences [11][12][13][14][15][16], which suggests that these parameters especially influence the biodiversity of such systems.…”

Section: Introductionmentioning

confidence: 99%

Lake-depth related pattern of genetic and morphological diatom diversity in boreal Lake Bolshoe Toko, Eastern Siberia

et al. 2020

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Large, old and heterogenous lake systems are valuable sources of biodiversity. The analysis of current spatial variability within such lakes increases our understanding of the origin and establishment of biodiversity. The environmental sensitivity and the high taxonomic richness of diatoms make them ideal organisms to investigate intra-lake variability. We investigated modern intra-lake diatom diversity in the large and old sub-arctic Lake Bolshoe Toko in Siberia. Our study uses diatom-specific metabarcoding, applying a short rbcL marker combined with next-generation sequencing and morphological identification to analyse the diatom diversity in modern sediment samples of 17 intra-lake sites. We analysed abundance-based compositional taxonomic diversity and generic phylogenetic diversity to investigate the relationship of diatom diversity changes with water depth. The two approaches show differences in taxonomic identification and alpha diversity, revealing a generally higher diversity with the genetic approach. With respect to beta diversity and ordination analyses, both approaches result in similar patterns. Water depth or related lake environmental conditions are significant factors influencing intra-lake diatom patterns, showing many significant negative correlations between alpha and beta diversity and water depth. Further, one near-shore and two lagoon lake sites characterized by low (0-10m) and medium (10-30m) water depth are unusual with unique taxonomic compositions. At deeper (>30m) water sites we identified strongest phylogenetic clustering in Aulacoseira, but generally much less in Staurosira, which supports that water depth is a strong environmental filter on the Aulacoseira communities. Our study demonstrates the utility of combining analyses of genetic and morphological as well as phylogenetic diversity to decipher compositional and generic phylogenetic patterns, which are relevant in understanding intra-lake heterogeneity as a source of biodiversity in the sub-arctic glacial Lake Bolshoe Toko.

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Section: Introductionmentioning

confidence: 99%

Lake-depth related pattern of genetic and morphological diatom diversity in boreal Lake Bolshoe Toko, Eastern Siberia

et al. 2020

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“…In most of the phylogenetic trees constructed so far, the Toxariids relate to the branch comprised of polar centric diatoms 5 , 8 , 9 , 11 , 12 , 33 – 35 ,present study . In one case, however, Ardissonea formosa (Hantzsch) Grunow was found to be sister to araphid pennates 36 . Polar centric diatoms correspond to Clade 2a in early studies by Medlin and colleagues, and now recognized at the class level as the Mediophyceae 5 , 37 – 39 .…”

Section: Discussionmentioning

confidence: 95%

Ardissonea crystallina has a type of sexual reproduction that is unusual for centric diatoms

Давидович

Давидович²,

Podunay³

et al. 2017

Sci Rep

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Molecular phylogenetic analyses place Ardissonea crystallina (C. Agardh) Grunow and all Toxariids among the bi- and multipolar centric diatoms, almost always recovered as a derived lineage sister to Lampriscus. In all centrics where sexual reproduction has been documented, oogamy, with larger immobile eggs and smaller flagellated sperm has been observed. We were able to initiate both homothallic and heterothallic reproduction in A. crystallina. The heterothallic reproduction turned out to be non-oogamous; gametes were more or less equal in size but no flagellated cells were detected. At the same time, two mating types (“male” and “female”) were recognized by the distinct morphology and behaviour of the gametes. While no flagella were observed, periodically thin cytoplasmic projections arose on the surface of the “male” gametes. These projections similar to those found in some pennate diatoms facilitated contact with the “female” cells. In each gametangial cell, regardless of the mating type, only one gamete was formed. Thus, the Toxariids may represent a unique evolutionary group, at least in respect to their reproductive biology. The hypothesis discussed is that non-oogamous mode of reproduction could have evolved in Ardissonea (and possibly in other Toxariids) independently of the pennate lineage of diatoms.

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“…The revision of the genera Fragilaria , Synedra , Ulnaria [ 37 – 39 ] being still in progress, we would rather use in our paper the species names S . ulna subsp.…”

Section: Methodsmentioning

confidence: 99%

A family of silicon transporter structural genes in a pennate diatom Synedra ulna subsp. danica (Kütz.) Skabitsch

Marchenkov

Petrova

et al. 2018

PLoS ONE

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Silicon transporters (SIT) are the proteins, which capture silicic acid in the aquatic environment and direct it across the plasmalemma to the cytoplasm of diatoms. Diatoms utilize silicic acid to build species-specific ornamented exoskeletons and make a significant contribution to the global silica cycle, estimated at 240 ±40 Tmol a year. Recently SaSIT genes of the freshwater araphid pennate diatom Synedra acus subsp. radians are found to be present in the genome as a cluster of two structural genes (SaSIT-TD and SaSIT-TRI) encoding several concatenated copies of a SIT protein each. These structural genes could potentially be transformed into “mature” SIT proteins by means of posttranslational proteolytic cleavage. In the present study, we discovered three similar structural SuSIT genes in the genome of a closely related freshwater diatom Synedra ulna subsp. danica. Structural gene SuSIT1 is identical to structural gene SuSIT2, and the two are connected by a non-coding nucleotide DNA sequence. All the putative “mature” SITs contain conserved amino acid motifs, which are believed to be important in silicon transport. The data obtained suggest that the predicted “mature” SIT proteins may be the minimal units necessary for the transport of silicon is S. ulna subsp. danica. The comparative analysis of all available multi-SITs has allowed us to detect two conservative motifs YQXDXVYL and DXDID, located between the “mature” proteins. Aspartic acid-rich DXDID motif can, in our opinion, serve as a proteolysis site during the multi-SIT cleavage. The narrow distribution of the distances between CMLD and DXDID motifs can serve as additional evidence to the conservation of their function.

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Morphological and molecular evidence support description of two new diatom species from the genus Ulnaria in Lake Baikal

Cited by 27 publications

References 34 publications

Lake-depth related pattern of genetic and morphological diatom diversity in boreal Lake Bolshoe Toko, Eastern Siberia

Lake-depth related pattern of genetic and morphological diatom diversity in boreal Lake Bolshoe Toko, Eastern Siberia

Ardissonea crystallina has a type of sexual reproduction that is unusual for centric diatoms

A family of silicon transporter structural genes in a pennate diatom Synedra ulna subsp. danica (Kütz.) Skabitsch

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