Morphological and molecular charaterisation of Pratylenchus parazeae n. sp. (Nematoda: Pratylenchidae) parasitizing sugarcane in China

Wang, Honghong; Zhuo, Kan; Ye, Weimin; Liao, Jinling

doi:10.1007/s10658-015-0674-z

Cited by 31 publications

(15 citation statements)

References 38 publications

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“…However, accurate diagnose of Pratylenchus spp. needs to be supported by means of morphological and molecular techniques (Subbotin et al 2008;De Luca et al 2011;Wang et al 2015;Nguyen et al 2017;Divsalar et al 2018). Among the DNA barcoding markers, the 28S rDNA (Carta et al 2001;Al-Banna et al 2004;De Luca et al 2004, 2011Subbotin et al 2005Subbotin et al , 2008 and COI of mtDNA (Singh et al 2018) have been used for Pratylenchus spp.…”

Section: Introductionmentioning

confidence: 99%

Molecular and morphological variation of the root-lesion nematode Pratylenchus neglectus

et al. 2018

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During a survey of plant-parasitic nematodes in Iran, five populations of Pratylenchus neglectus were studied by means of morphological and molecular characterization. The results showed that body length has high significant correlation with morphometric data indices such as a (r = 0.487; p ≤ 0.01), b (r = 0.355; p ≤ 0.05), c (r = 0.527; p ≤ 0.01), stylet (r = 0.625; p ≤ 0.01), excretory pore (r = 0.750; p ≤ 0.01), overlapping (r = 0.434; p ≤ 0.01) and tail length (r = 0.447; p ≤ 0.05). However, the highest correlation was detected between pharynx overlapping and postvulval uterine sac (r = −0.935; p ≤ 0.01). A molecular study of the P. neglectus populations using 28S rDNA showed close relationships of the Iranian, UK (KX683377), South Korea (KY468842) and China (JX046968). The COI of mtDNA analysis showed close relationship of the Iranian P. neglectus with the Chineese populations (KX349423; KY424105). The results indicated monophyly of P. neglectus. Illustration and measurement tables are provided for the species.

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Section: Introductionmentioning

confidence: 99%

Molecular and morphological variation of the root-lesion nematode Pratylenchus neglectus

et al. 2018

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“…a. the FAM signal showing specificity for P. neglectus, Gsy24S, Sd12922, Sdwg and Xz9 represent four populations of P. neglectus; nontarget nematodes including P. thornei, P. coffeae, P. brachyurus, P. loosi, P. parazeae, P. scribneri, P. vulnus, P. zeae, Meloidogyne incognita, M. enterolobii, Heterodera fengi, Aphelenchoides besseyi and Caenorhabditis elegans; b. the ROX signal showing specificity for P. thornei. Gsy24L and Xjdm represent two populations of P. thornei; non-target nematodes including P. neglectus, P. coffeae, P. brachyurus, P. loosi, P. parazeae, P. scribneri, P. vulnus, P. zeae, M. incognita, M. enterolobii, H. fengi, A. besseyi and C. elegans Pratylenchus species (De Luca et al 2011;Wang et al 2015), making it difficult to design species-specific primers. Intraspecific variations in the ITS-rDNA of P. neglectus and P. thornei could reach 7% and 6% respectively (De Luca et al 2011).…”

Section: Discussionmentioning

confidence: 99%

Duplex real-time quantitative PCR for simultaneous detection and quantification of Pratylenchus neglectus and P. thornei

Lin

Tao

Wang³

et al. 2020

Eur J Plant Pathol

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Pratylenchus neglectus and P. thornei are economically important migratory plant endoparasitic nematodes and are widely found in a wide variety of crops. Based on 28 s rDNA D2D3 expansion region, a duplex real-time quantitative (qPCR) assay was developed to simultaneously detect and quantify these two nematodes. The qPCR system included two pairs of primers and two Taqman probes, that is the P. neglectus-specific primer pair 28sPnF5/28sPnR7 and probe pntaq3 and the P. thornei-specific primer pair 28sPtF1/28sPtR1 and probe pttaq3. The optimal conditions of the qPCR assay were 20 μL reaction volume comprising 10 μL THUNDERBIRD Probe qPCR Mix, 0.2 μM of primer 28sPnF5 and 28sPnR7, 0.25 μM of primer 28sPtF1and 28sPtR1, 0.1 μM of probe pntaq3 and 0.08 μM of probe pttaq3, 1 μL template DNA. And the amplification program were 95°C for 2 min, 40 cycles of 95°C for 10s, 65°C for 25 s. The specificity of the qPCR assay was confirmed by the lack of fluorescence signal from non-target nematode populations including seven other Pratylenchus species and five other nematode species. The assay was very sensitive as it can detect a single target nematode and a single target nematode mixed with up to 100 individuals of non-target nematodes. A dilution series from P. neglectus and P. thornei DNAs resulted in two standard curves respectively showing a highly significant linearity between the Cycle threshold (Ct) values and the dilution rates. This study is the first to provide a molecular tool for simultaneous detection and quantification of P. neglectus and P. thornei.

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“…Some of these have been used separately or in combination with traditional methods to accurately characterize and to fingerprint Pratylenchus species (Waeyenberge et al, 2000;Waeyenberge et al, 2009;De Luca et al, 2011). The use of the former approach has been very successful because of the inter-genetic variation in the ITS1 and ITS2 sequences of different species, notwithstanding any intra-genetic variations in individual nematodes of the same species (Duncan et al, 1999;Waeyenberge et al, 2000;Waeyenberge et al, 2009;Wang et al, 2015). During the analyses of our data, it was surprising to note that although there…”

Section: Discussionmentioning

confidence: 99%

Serendipitous identification of Pratylenchus curvicauda from the grainbelt of Western Australia

Begum

Fosu-Nyarko

Sharma

et al. 2019

Journal of Nematology

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A Pratylenchus species identified during a survey of Pratylenchus quasitereoides incidence at four locations of the grainbelt of Western Australia is described. Morphological and morphometric features indicated the previously undescribed morphotypes in nematode mixtures encountered were conspecific to P. curvicauda, and were clearly distinguishable from nine common Pratylenchus spp. Typical features of P. curvicauda were its body length (415-540 µm), which was curved to a c-shaped with a maximum body diameter of 20 µm, and the nature of its tail; 34 µm long, 2.8 µm wide at the anus and a typical ventrally arcuate with a round terminus. Sequenced for the first time, the sequences of the partial 18S-ITS1-5.8S-ITS2-partial 28S (80 clones, 14 individual nematodes) and the 28S-D3 (17 clones) regions of the rDNA of P. curvicauda had overall mean distances of 0.013 and 0.085, respectively. Phylogenetic analyses with sequences of both segments of the rDNA clearly showed the P. curvicauda isolates as monophyletic, distinct from ca 40 Pratylenchus species. Notably, it was distinct from Pratylenchus species present in Australia including P. quasitereoides and a Western Australia isolate of P. thornei. Further research into the biology of P. curvicauda is needed to facilitate development of strategies for its management, if it is an important pest.

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Morphological and molecular charaterisation of Pratylenchus parazeae n. sp. (Nematoda: Pratylenchidae) parasitizing sugarcane in China

Cited by 31 publications

References 38 publications

Molecular and morphological variation of the root-lesion nematode Pratylenchus neglectus

Molecular and morphological variation of the root-lesion nematode Pratylenchus neglectus

Duplex real-time quantitative PCR for simultaneous detection and quantification of Pratylenchus neglectus and P. thornei

Serendipitous identification of Pratylenchus curvicauda from the grainbelt of Western Australia

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