Morphological and molecular characters of Cherax quadricarinatus (von Martens, 1868) from Sermo Reservoir and Tambakboyo Retention Basin, Daerah Istimewa Yogyakarta, Indonesia

Abstract

The Australian red claw freshwater crayfish, Cherax quadricarinatus is one of the most widely distributed and cultivated freshwater crayfish due to its high tolerance towards various environmental conditions. Native to North Australia and South Papua New Guinea, this crayfish was found in Tambakboyo Retention Basin in 2016 and Sermo Reservoir in 2019. This research was aimed to identify the morphological and molecular characters of C. quadricarinatus collected from Sermo Reservoir and Tambakboyo Retention Basin, Yogyakarta. The genetic information of the samples was compared to Australian red claw freshwater crayfish currently available. The methodology used for this research are morphological, morphometrical, meristic identification, and molecular identification using the PCR method. The primer used to be 1471 primers as the forward primer and 1472 primers as the reverse primer. In conclusion, all six specimens obtained were identified to be C. quadricarinatus. Morphological analysis using UPGMA showed that all specimens were formed one big cluster and has the highest similarity index (1.00). Molecular analysis using BLAST showed that specimen from Sermo Reservoir was 98.96% identical to C. quadricarinatus and specimen from Tambakboyo Retention Basin was 100% identical to C. quadricarinatus. Thus concluding that based on their morphological and molecular character, all samples of this study were C. quadricarinatus. This finding also contributes to the distribution information of C. quadricarinatus in Daerah Istimewa Yogyakarta

Molecular characterization of genus Tor from Indonesia based on 16S rRNA

“…Nowadays, one of the best choices to protect a species is to preserve its genetic resources and molecular characterization besides the preservation of the ecosystem (Nugroho & Rahayu, 2015). Molecular characterization are the basic concept for building classifications, understanding evolutionary relationships between organisms, and reaching correct identification methods (Eprilurahman et al, 2021;Glon et al, 2018;Skuza et al, 2016). The information of molecular characters can be used an effort to increase the number of populations and prevent inbreeding that is closely related because it will reduce the genetic diversity of species.…”