Morphological and molecular characterization of <i>Paratylenchus beltsvillensis</i> n. sp. (Tylenchida: Paratylenchidae) from the rhizosphere of pine tree (<i>Pinus virginiana</i> Mill) in Maryland, USA

Kantor, Mihail; Handoo, Zafar A.; Subbotin, Sergei A.; Bauchan, Gary R.; Mowery, Joseph

doi:10.21307/jofnem-2021-079

Cited by 4 publications

(4 citation statements)

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“…Forty-four sequences belonging to two nuclear rRNA and one mtRNA markers were generated in this study: 24 D2-D3, 9 18S, and 11 partial COI sequences. The results corroborate previous studies [5,6,8,42,[46][47][48] in finding the utility of this integrative approach and molecular data for species discrimination in Paratylenchus. Our findings are in accordance with the results of recent studies [5,6], since they showed that the D2-D3 of the 28S rRNA and the COI region within mtRNA were the most decisive, precise, and reliable molecular markers for discriminating among species and for the diagnosis at species level within Paratylenchus genus.…”

Section: Discussionsupporting

confidence: 91%

“…Members of the genus Paratylenchus Micoletzky, 1922 sensu lato, commonly known as pin nematodes, belong to the family Tylenchulidae Skarbilovich, 1947 (superfamily Criconematoidea Taylor, 1936 (Geraert, 1966); infraorder Tylenchomorpha De Ley & Blaxter, 2002; suborder Tylenchina Chitwood, 1950; order Rhabditida) [2][3][4]. According to morphological features and new molecular phylogenetic data [5][6][7][8], currently members belonging to Paratylenchus sensu lato are divided on two subgroups without taxonomic validity: (i) Paratylenchus species with a straight and short stylet, apparently the Iberian Peninsula were with Spanish populations of pin nematodes [5,6,38,40,41], with exception of Lima [43] and Macara [44]. To our knowledge, no previous studies of detailed descriptions included measurements and/or molecular characterizations of Portuguese populations of pin nematodes.…”

Section: Introductionmentioning

confidence: 99%

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Six First Reports of Pin Nematodes from Portugal, with an Update of the Systematics, Genetic Diversity, and Phylogeny of the Genus Paratylenchus (Nematoda: Tylenchulidae)

et al. 2022

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Pin nematodes (Paratylenchus spp.) currently comprise 132 species of polyphagous plant ectoparasites with at least seven species that are plant pathogenic emphasizing the need for correct identification to establish an appropriate management strategy. Sequences of highly conserved regions of ribosomal and mitochondrial RNA genes are a powerful species-level diagnostic tool within Tylenchulidae. A nematological survey was conducted from 2019 to 2021 in commercial vineyards distributed in four major wine-producing regions in the Central and South Portugal. Nine populations of Paratylenchus spp. were isolated from the rhizosphere of grapevines that were characterized from morphological data and molecular phylogenetic analysis using two rRNA genes (D2–D3 expansion segments of the 28S, and partial 18S) and a region partial of the COI mtRNA gene. Contrasting morphological hypotheses with molecular data provided rapid detection of six species, specifically P. goodeyi, P. hamatus, P. pedrami, P. tenicaudatus, P. variabilis, and P. veruculatus. Bayesian inference (BI) phylogenetic trees for these molecular markers established phylogenetic relationships underscore the importance of using genomic and molecular phylogenetic data for accurate pin nematode identification at the species level. To our knowledge, this is the first report of Paratylenchus spp. in Portugal, extending the geographical distribution of these species in the Mediterranean Basin, and the first record of P. goodeyi, P. pedrami, P.variabilis, P. veruculatus and P. tenicaudatus parasitizing grapevine.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Six First Reports of Pin Nematodes from Portugal, with an Update of the Systematics, Genetic Diversity, and Phylogeny of the Genus Paratylenchus (Nematoda: Tylenchulidae)

et al. 2022

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“…These clades are primarily equivalent with previous studies on Paratylenchus spp. phylogeny [ 3 , 4 , 5 , 6 , 9 , 45 ].…”

Section: Resultsmentioning

confidence: 99%

DNA Barcoding and Morphometry Reveal Further Cryptic Bio-Diversity within the Pin Nematode Genus Paratylenchus (Nematoda: Tylenchulidae)

Palomares-Rius

Archidona-Yuste

Clavero-Camacho

et al. 2022

Plants

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Paratylenchus species are obligate ectoparasitic nematodes on cultivated and wild herbaceous and woody plants occupying numerous soil categories. Several species may cause damage to several crops (viz. P. dianthus, P. enigmaticus, P. microdorus, P. hamatus and P. epacris on carnation, lettuce, rose and walnut, respectively). This investigation proves and emphasizes the relevance of applying integrative taxonomy for the accurate detection of Paratylenchus species in mountainous wild environments in the Malaga province, Southern Spain. This research analyzed 45 soil samples of maritimus pine and one of green heather in southern Spain and identified fourteen Paratylenchus species, two of them are described herein as new species (P. paraaonli sp. nov., P. plesiostraeleni sp. nov.), six of them were first reports for Spain (P. canchicus, P. nainianus, P. neonanus, P. salubris, Paratylenchus sp. 2 SAS, and P. wuae), and six species (P. caravaquenus, P. microdorus, P. nanus, P. neoamblycephalus, P. sheri, and P. variabilis) have been already reported in Spain. Accordingly, these data increase the biodiversity of pin nematodes in Spain comprising a total of 47 species (33.1% out of 142 total species of this genus). Phylogenetic analyses based on ribosomal and mitochondrial markers (D2-D3, ITS, and partial COI) resulted in a consistent position for the newly described Paratylenchus species in this study (P. plesiostraeleni sp. nov., P. paraaonli sp. nov.). Paratylenchus plesiostraeleni sp. nov. grouped in a separated subclade as unequivocal species from the P. straeleni-complex species (including P. straeleni and P. parastraeleni), and P. paraaonli sp. nov. clustered with P. vitecus, but clearly separate from this species. This study indicates that Paratylenchus species diversity in natural environments may be higher than expected, and this study may help in accurate identifications.

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“…The newly obtained sequences of the D2-D3 expansion segments of the 28S rRNA, the ITS of the rRNA, and partial COI mtDNA genes were aligned using ClustalX 1.83 [91] with corresponding published gene sequences [13,14,23,24,26,30,[65][66][67]69,71,80,[92][93][94][95][96][97]. Outgroup taxa for each dataset were chosen based on previously published data [30].…”

Section: Phylogenetic and Sequence Analysismentioning

confidence: 99%

Morphological and Molecular Diversity among Pin Nematodes of the Genus Paratylenchus (Nematoda: Paratylenchidae) from Florida and Other Localities and Molecular Phylogeny of the Genus

Álvarez-Ortega

Subbotin

Wang

et al. 2023

Plants

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Pin nematodes (Paratylenchus spp.) are root parasites found worldwide. They have different life cycles and feeding habits and can damage a wide range of plants. A remarkable diversity of pin nematode species was found in soil samples from Florida and other states of the USA, Canada, and Spain. Using integrative taxonomy, two new species (Paratylenchus hawaiiensis sp. n. and P. roboris sp. n.), six valid species (Paratylenchulus acti, P. aquaticus, P. goldeni, P. paralatescens, P. minutus (=P. shenzhenensis syn. n.), and P. straeleni), and two undescribed species were identified from Florida; P. goldeni, P. hamatus, P. hamicaudatus, P. holdemani, and P. pedrami were found in California, P. minutus in Hawaii, P. goldeni in Oregon and Washington, and one new species, Paratylenchus borealis sp. n., in Alaska. Outside the USA, Paratylenchus projectus was detected in samples from Canada and Spain as well as P. holdemani and Paratylenchus sp. from Spain. The pin nematode species from Belgium and Russia identified in former studies as Paratylenchus sp. F was herein described as a new species with the name of P. borealis sp. n., using a population from Alaska. Previously reported molecular type A of P. aquaticus from Hawaii was reclassified as P. hawaiiensis sp. n., using a population from Florida. Paratylenchus roboris sp. n. from Florida has obese sedentary females with a stylet 63–71 µm long. The results of the molecular analysis of P. shenzhenensis from Florida and China indicated that it was conspecific with P. minutus from Hawaii and considered here as its junior synonym. New 26 D2–D3 expansion segments of 28S rRNA, 17 ITS rRNA, and 20 COI gene sequences were obtained in this study. Phylogenetic relationships of Paratylenchus are reconstructed using the D2–D3 of 28S rRNA, ITS rRNA, and COI gene sequences. Congruence of molecular and morphological evolution and species identification problems are discussed. Obese females were found in two major clades of Paratylenchus. The problem of reference materials is discussed, and it is proposed to make more efforts to collect topotype materials of known Paratylenchus species for molecular study.

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Morphological and molecular characterization of Paratylenchus beltsvillensis n. sp. (Tylenchida: Paratylenchidae) from the rhizosphere of pine tree (Pinus virginiana Mill) in Maryland, USA

Abstract: The pin nematode, Paratylechus beltsvillensis n. sp. collected from rhizosphere soil of a Virginia pine tree (Pinus virginiana Mill) growing in

Cited by 4 publications

References 25 publications

Six First Reports of Pin Nematodes from Portugal, with an Update of the Systematics, Genetic Diversity, and Phylogeny of the Genus Paratylenchus (Nematoda: Tylenchulidae)

Six First Reports of Pin Nematodes from Portugal, with an Update of the Systematics, Genetic Diversity, and Phylogeny of the Genus Paratylenchus (Nematoda: Tylenchulidae)

DNA Barcoding and Morphometry Reveal Further Cryptic Bio-Diversity within the Pin Nematode Genus Paratylenchus (Nematoda: Tylenchulidae)

Morphological and Molecular Diversity among Pin Nematodes of the Genus Paratylenchus (Nematoda: Paratylenchidae) from Florida and Other Localities and Molecular Phylogeny of the Genus

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