Morphological and molecular characterization of Tovellia cf. aveirensis (Dinophyceae) from Jiulong River, China

Luo, Zhaohe; You, Xuejing; Mertens, Kenneth Neil; Gu, Haifeng

doi:10.1127/nova_hedwigia/2016/0339

Cited by 3 publications

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“…Gymnodinium dodgei Sarma & Shyam) have been transferred based on cyst morphology (Luo et al . ), but cyst‐motile cell relationship of additional Gymnodinium species is needed. Here we isolate two cysts from surface sediments collected from Plastic Lake, Canada and establish the cyst‐motile cell relationship of a new Gymnodinium species.…”

Section: Introductionmentioning

confidence: 99%

Cyst‐motile stage relationship and molecular phylogeny of a new freshwater dinoflagellate Gymnodinium plasticum from Plastic Lake, Canada

Wang

Luo

Mertens

et al. 2017

Phycological Research

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The dinophyceaen genus Gymnodinium was established with the freshwater species G. fuscum as type. According to Thessen et al. (2012), there are 268 species, with the majority marine species. In recently published molecular phylogenies based on ribosomal DNA sequences, Gymnodinium is polyphyletic. Here, a new freshwater Gymnodinium species, G. plasticum, is described from Plastic Lake, Ontario, Canada. Two strains were established by incubating single cysts, and their morphology was examined with light microscopy and scanning electron microscopy. The cyst had a rounded epicyst and hypocyst with a wide cingulum and smooth surface. Vegetative cells were characterized by an elongated nucleus running vertically and a deep sulcal intrusion. The apical structure complex was horseshoe-shaped and consisted of two pronounced ridges with a deep internal groove, encircling 80% of the apex. Small subunit ribosomal DNA (SSU rDNA), large subunit ribosomal DNA (LSU rDNA) and internal transcribed spacer (ITS) sequences were obtained from cultured strains. Molecular phylogeny based on concatenated SSU, LSU and ITS sequences supports the monophyly of the Gymnodiniales sensu stricto clade but our results suggest that many Gymnodinium species might need reclassification. Gymnodinium plasticum is closest to Dissodinium pseudolunula in our phylogeny but distant from the type species G. fuscum, as are the other gymnodiniacean taxa.

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Section: Introductionmentioning

confidence: 99%

Cyst‐motile stage relationship and molecular phylogeny of a new freshwater dinoflagellate Gymnodinium plasticum from Plastic Lake, Canada

Wang

Luo

Mertens

et al. 2017

Phycological Research

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“…The presence of a cyst of the same general type was the justification for the recent transfer of T. dodgei (Sarma and Shyam) K. N. Mertens and H. Gu from the genus Gymnodinium (Luo et al. ; Sarma and Shyam ). The cysts of T. dodgei were also red, but the motile cells were more elongate and the sulcus invaded the epicone, creating a distinct plough‐shaped depression.…”

Section: Discussionmentioning

confidence: 99%

“…This general morphology appears closer to the cysts of T. aveirensis, which, however, showed a marked paracingulum and spines twice as long when mature; and, to some extent, to cysts of T. paldangensis, which had much fewer and shorter spines (Moestrup and Calado 2018;Pandeirada et al 2014). The presence of a cyst of the same general type was the justification for the recent transfer of T. dodgei (Sarma and Shyam) K. N. Mertens and H. Gu from the genus Gymnodinium (Luo et al 2016;Sarma and Shyam 1974). The cysts of T. dodgei were also red, but the motile cells were more elongate and the sulcus invaded the epicone, creating a distinct plough-shaped depression.…”

Section: Discussionmentioning

confidence: 99%

Studies on Woloszynskioid Dinoflagellates X: Ultrastructure, Phylogeny and Colour Variation in Tovellia rubescens n. sp. (Dinophyceae)

Pandeirada

Craveiro

Daugbjerg

et al. 2019

J Eukaryotic Microbiology

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The external morphology and internal cell fine structure of a new species of Tovelliaceae, Tovellia rubescens n. sp., is described. Phylogenetic analyses based on partial LSU rDNA sequences place the new species in a clade containing Tovellia species that accumulate red pigments and identify T. aveirensis as its closest known relative. Cells of T. rubescens n. sp. were mostly round and had the cingulum located near the middle, with its ends displaced about one cingular width. Small numbers of distinctly flat cells appeared in culture batches; their significance could not be determined. Cells of the new species in culture batches progressively changed from a yellowish‐green, mainly due to chloroplast colour, to a reddish‐brown colour that appeared associated with lipid bodies. The switch to a reddish colour happened earlier in batches grown in medium lacking sources of N or P. Pigment analyses by HPLC‐MS/MS revealed the presence of astaxanthin and astaxanthin‐related metabolites in the new species, but also in T. aveirensis, in which a reddish colour was never observed. The chloroplast arrangement of T. rubescens n. sp. resembled that of T. aveirensis, with lobes radiating from a central pyrenoid complex. The flagellar apparatus and pusular system fell within the general features described from other Tovelliaceae. A row of microtubules interpretable as a microtubular strand of the peduncle was present. Spiny resting cysts with red contents and an ITS sequence identical to that of cultured material of the new species were found in the original locality.

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Studies on woloszynskioid dinoflagellates VIII: life cycle, resting cyst morphology and phylogeny ofTovellia rinoi sp. nov. (Dinophyceae)

et al. 2017

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Morphological and molecular characterization of Tovellia cf. aveirensis (Dinophyceae) from Jiulong River, China

Cited by 3 publications

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Cyst‐motile stage relationship and molecular phylogeny of a new freshwater dinoflagellate Gymnodinium plasticum from Plastic Lake, Canada

Cyst‐motile stage relationship and molecular phylogeny of a new freshwater dinoflagellate Gymnodinium plasticum from Plastic Lake, Canada

Studies on Woloszynskioid Dinoflagellates X: Ultrastructure, Phylogeny and Colour Variation in Tovellia rubescens n. sp. (Dinophyceae)

Studies on woloszynskioid dinoflagellates VIII: life cycle, resting cyst morphology and phylogeny ofTovellia rinoi sp. nov. (Dinophyceae)

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