Morphological and molecular characterization of <i>Magnaporthe oryzae</i> from Chhattisgarh

Panda, Gayatree; Sahu, Chinmayee; Yadav, Manoj Kumar; Aravindan, S; Ngangkham, Umakanta; Raghu, S.; Prabhukarthikeyan, S.R.; Lenka, Subasini; Tiwari, Jitendra Kumar; Kar, Sonali; Jena, Mayabini

doi:10.5958/2249-5266.2017.00045.5

Cited by 13 publications

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“…Virulence analysis indicated three virulence types, and the majority of the isolates (53.8%) were highly virulent. Interestingly, most virulent isolates formed greyish colonies, similar to the previous report from India (Panda et al, 2017).…”

Section: Discussionsupporting

confidence: 89%

“…In this study, significant morphological variability was observed among eco-distinct isolates in their colony colour, appearance, and forms. The colour of the colony was varied from grey to white, and similar colony features were reported previously from India (Panda et al, 2017;Singh et al, 2018;. However, we could not find any correlation between the morphological types with the ecosystem origin of isolates.…”

Section: Discussionsupporting

confidence: 82%

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Morpho‐molecular diversity and avirulence genes distribution among the diverse isolates of Magnaporthe oryzae from Southern India

Chittaragi

Pramesh

Naik

et al. 2021

J of Applied Microbiology

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Aims:To investigate the diversity of eco-distinct isolates of Magnaporthe oryzae for their morphological, virulence and molecular diversity and relative distribution of five Avr genes. Methods and Results:Fifty-two M. oryzae isolates were collected from different rice ecosystems of southern India. A majority of them (n = 28) formed a circular colony on culture media. Based on the disease reaction on susceptible cultivar (cv. HR-12), all 52 isolates were classified in to highly virulent (n = 28), moderately virulent (n = 11) and less-virulent (13) types. Among the 52 isolates, 38 were selected for deducing internal transcribed spacer (ITS) sequence diversity. For deducing phylogeny, another set of 36 isolates from other parts of the world was included, which yielded two distinct phylogenetic clusters. We identified eight haplotype groups and 91 variable sites within the ITS sequences, and haplotype-group-2 (Hap_2) was predominant (n = 24). The Tajima's and Fu's Fs neutrality tests exhibited many rare alleles. Furthermore, PCR analysis for detecting the presence of five Avr genes in the different M. oryzae isolates using Avr gene-specific primers in PCR revealed that Avr-Piz-t, Avr-Pik, Avr-Pia and Avr-Pita were present in 73.68%, 73.68%, 63.16% and 47.37% of the isolates studied, respectively; whereas, Avr-Pii was identified only in 13.16% of the isolates.Conclusions: Morpho-molecular and virulence studies revealed the significant diversity among eco-distinct isolates. PCR detection of Avr genes among the M. oryzae population revealed the presence of five Avr genes. Among them, Avr-Piz-t, Avr-Pik and Avr-Pia were more predominant. Significance and Impact of the Study:The study documented the morphological and genetic variability of eco-distinct M. oryzae isolates. This is the first study demonstrating the distribution of the Avr genes among the eco-distinct population of M. oryzae from southern India. The information generated will help plant breeders to select appropriate resistant gene/s combinations to develop blast disease-resistant rice cultivars. 7 MF716846.1 M. oryzae isolate AMA UOC-01 8 KT693184.1 M. oryzae strain UPM-PO Malaysia 9 KM249937.1 M. oryzae strain PO-FA01 10 MW042178.1 P. oryzae strain Pos-3 China 11 MW042177.1 P. oryzae strain Pos-2 12 MT588875.1 P. grisea isolate DW-2016-2 13 MW042176.1 P. oryzae strain Pos-1 14 MG642970.1 M. e grisea 15 MF946553.1 Pyricularia sp. strain F2 United Kingdom 16 MN203028.1 P. oryzae isolate T49 17 FN555121.1 M. oryzae 18 JX469384.1 M. oryzae culture-collection NFCCI:2605 India (NE) 19 MF678836.1 M. oryzae isolate Gudalur India (TN) 20 MF669475.1 M. oryzae isolate Nagaranai 21 MF668695.1 M. oryzae isolate Aduthurai 22 MF668692.1 M. oryzae isolate Maduranthakam 23 KM816799.1 M. oryzae strain w11-65 18S Korea 24 KM816801.1 M. oryzae strain w11-110 25 KM816797.1 M. oryzae strain w11-58 26 KM816796.1 M. oryzae strain w11-23 27 AB269937.1 M. grisea genes Japan 28 MT103091.1 P. oryzae isolate Mf3 Iran 29 MT103090.1 P. oryzae isolate Mb3 30 LC552959.1

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 82%

Morpho‐molecular diversity and avirulence genes distribution among the diverse isolates of Magnaporthe oryzae from Southern India

Chittaragi

Pramesh

Naik

et al. 2021

J of Applied Microbiology

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“…Rice is the most valuable, and primary, food crop for more than 50% of the world's population (Khush, 2005). It is the most important staple food for people living in rural and urban areas of humid and sub-humid Asia and accounts for 23% of the calories consumed by the global human population (Wilson & Talbot, 2009;Panda et al, 2017). Many biotic and abiotic factors influence production and productivity of rice.…”

Section: Introductionmentioning

confidence: 99%

Molecular identification of blast resistance genes in rice landraces from northeastern India

et al. 2018

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Rice blast disease caused by the fungus Magnaporthe oryzae is one of the most devastating diseases causing huge losses worldwide. In the present study, major blast resistance genes were investigated in landraces originating from northeastern India. Based on phenotypic evaluation, 288 landraces were classified into three distinct groups: resistant (75), moderately resistant (127) and susceptible (86). The genetic frequencies of the 18 major blast resistance genes were between 6.2% and 27.4%, with only two genotypes possessing a maximum of nine blast resistance genes. The cluster and population structure analysis grouped the landraces into two groups. Through principal coordinate analysis, the scatter plots partitioned the resistant and moderately resistant landraces into different groups. Analysis of molecular variance showed maximum (96%) diversity within populations and least (4%) diversity between populations. Association analysis identified six markers, CRG4_2, RM72, tk59‐2, pi21_79‐3, RM1233 and RM6648, that are significantly associated with blast disease and explained a phenotypic variance of 1.1–6.5%. The associated genes could be used in marker‐assisted rice breeding programmes for gene pyramiding to develop rice varietal resistance against blast disease. The present study represents a valuable blast resistance genetic resource that could be used for identification of new R genes, donors for blast resistance, and genomic studies.

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“…Cultural characteristics between leaf blast isolates were recorded and variability differentiated. Panda et al (2017) characterized twenty isolates of P. oryzae and categorized based on the variation in morphological characteristics viz., colony color, surface appearance and type of growth. The isolates showed few surface, downy, at with few mycelium and submerged growth with smooth and rough margins on OMA media.…”

Section: Discussionmentioning

confidence: 99%

Investigation on Cultural Characterization and Parasexual Recombination in Pyricularia Grisea, Causal Agent of Blast of Rice

Boyapati¹,

Barnwal²,

Kumar³

et al. 2021

Preprint

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Blast of rice caused by Pyricularia grisea is one of the most devastating diseases of rice. Because of importance of the disease and the fact that pyricularia grisea is considered to be notorious and model species. The variability in cultural characteristics of fifty isolates of P. grisea were taken from different regions of Jharkhand state. Out of fifty isolates of P. grisea, colony color of six isolates were found to be as greyish white color, three isolates were blackish grey, six isolates as white color, three isolates as whitish grey color, five isolates were whitish black and twenty seven isolates were recorded as blackish white in color. The growth pattern of 47 isolates of P. grisea showed circular growth pattern and three isolates have irregular growth pattern but elevation of the mycelium differs from flat to raised. Out of fifty isolates of P. grisea, sector formation was observed in seventeen isolates and no sector formation was observed in rest isolates. The radial growth of fifty isolates of P. grisea were ranged from 76.0 mm to 90.0 mm. Out of fifty isolates, group I included six isolates, group II and III included eleven and thirty three isolates, respectively. Parasexual recombination rarely causes genetic and phenotypic variation through hyphal anastomosis in India. Parasexual recombination is the principle cause in rice blast fungus and cause infection of resistant rice variety (IR-64) in India .

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Morphological and molecular characterization of Magnaporthe oryzae from Chhattisgarh

Cited by 13 publications

References 0 publications

Morpho‐molecular diversity and avirulence genes distribution among the diverse isolates of Magnaporthe oryzae from Southern India

Morpho‐molecular diversity and avirulence genes distribution among the diverse isolates of Magnaporthe oryzae from Southern India

Molecular identification of blast resistance genes in rice landraces from northeastern India

Investigation on Cultural Characterization and Parasexual Recombination in Pyricularia Grisea, Causal Agent of Blast of Rice

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