Morphological and molecular characterization of a new species Myxobolus gutturocola n. sp. (Myxozoa: Myxobolidae) from the throat of Hypophthalmichthys molitrix in China

Liu, J. M.; Zhang, J. Y.; Zhao, Yuanjun

doi:10.1007/s00436-019-06226-9

Cited by 9 publications

(6 citation statements)

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“…A holistic approach integrating molecular data, phenotypic features, tissue tropism and host specificity to identify and describe species has been widely accepted ( Zhao et al, 2013 ; Fiala, Bartošová-Sojková & Whipps, 2015 ; Liu, Yang & Zhao, 2016 ; Liu, Zhang & Zhao, 2019 ). Morphologically, although the mature myxospores of the present species closely resembles M. koi , M. tanakai , M. orissae , and M. parakoi , which all parasitize in C. carpio except for M. orissae , the morphological structural features are inconsistent more or less.…”

Section: Discussionmentioning

confidence: 99%

“…To the best of our knowledge, the different species with quite a resemblance in morphology can parasitize the same site of the same host. For example, Myxobolus paratoyamai Kato et al, 2017 , Myxobolus toyamai Kudo, 1917, Myxobolus tanakai Kato et al, 2017 , Myxobolus parakoi Liu et al, 2019 , and M. koi all parasitize the gill lamellae of C. carpio ( Kato et al, 2017 ; Liu, Zhang & Zhao, 2019 ; Yokoyama & Ogawa, 2015 ; Zhang et al, 2020 ). Therefore, the species recorded independently by Kudo and Nakai are probably homonymous and heterogeneous despite their similarity in morphology and infecting the same site of the same host.…”

Section: Introductionmentioning

confidence: 99%

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Morphological description and molecular identification ofMyxobolus dajiangensisn. sp. (Myxozoa: Myxobolidae) from the gill ofCyprinus carpioin southwest China

Wang

Zhang

Zhao

2022

PeerJ

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Background Myxosporean diversity is a hot topic since they are difficult to accurately identify and classify. Many Myxobolus parasites have been named as Myxobolus koi because of their similar morphological features with the species originally reported. However, the distinctions in fine morphological features, host specificity, and molecular data have given rise to the attention of researchers. Methods The classical morphometric and histological methods were used to describe the Myxobolus dajiangensis n. sp. in morphology. The common techniques in modern molecular biology and the methods of phylogenetic analyses were combined to identify the species. Results Plasmodia of interlamellar-vascular type were found in the vascular network of gill lamellae. Mature myxospores of M. dajiangensis n. sp. were elongated and pyriform from the frontal view. The myxospores were 14.8 ± 0.4 (13.9–15.6) µm in length, 8.0 ± 0.5 (7.2–9.1) µm in width, and 5.5 µm in thickness. The two polar capsules were pyriform and slightly different in length. The length of the larger polar capsules was 8.0 ± 0.4 (7.1–8.8) µm, and it was 7.4 ± 0.4 (6.1–8.0) µm for the smaller ones. The width of both polar capsules was 2.5 ± 0.2 (2.0–3.2) µm. The polar filaments within the polar capsules were each coiled nine to 11 turns. Comparative analysis of both the morphological and molecular data between the present speices and other similar species revealed that the present species is a novel species, Myxobolus dajiangensis n. sp. Also, M. koi (FJ710800) was misidentified and the congener with M. dajiangensis n. sp., depending on the secondary structures of SSU rRNA and phylogenetic analysis. Moreover, the cryptic species existed in the M. koi parasites.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Morphological description and molecular identification ofMyxobolus dajiangensisn. sp. (Myxozoa: Myxobolidae) from the gill ofCyprinus carpioin southwest China

Wang

Zhang

Zhao

2022

PeerJ

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“…Molecular characteristics, such as sequence similarity and variable sites, are usually regarded as important evidence for species identification and discrimination of closely related myxosporean species (Liu et al, 2019; Sanil et al, 2017; Zhao et al, 2013). Generalizing, when the similarity of the two sequences is less than 96.4% (Atkinson et al, 2015; Ferguson et al, 2008) or variable sites of intra‐species identification are more than ten (Zhao et al, 2013), it can be considered to be different species.…”

Section: Discussionmentioning

confidence: 99%

Description of Myxidiumpseudocuneiforme n. sp. (Myxosporea: Myxidiidae) from Cyprinuscarpio in China, with the resolution on a taxonomic dilemma of Myxidiumcuneiforme

Chen

Zhang

Whipps

et al. 2021

J Eukaryotic Microbiology

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Investigations on myxozoan parasites of fish from Chongqing in China, revealed two Myxidium cuneiforme‐like myxosporeans infecting the gallbladder of Cyprinus carpio carpio and Carassius auratus. We researched their myxospore morphology, and analyzed their genetic similarity and phylogenic relationships to other myxozoans based on small subunit ribosomal DNA (18S rDNA) sequences. Although both parasites recovered were morphologically similar, the myxosporean isolated from C. auratus was consistent in morphology to Myxidium cuneiforme, which was described from this host species. The parasite isolated from C. c. carpio had overlapping myxospore dimensions to M. cuneiforme, but on average, the polar capsules were not as long. More importantly, this parasite was genetically distinct from M. cuneiforme with 96.3% and 96.5% similarity in two sequences of 18S rDNA, and we propose the name Myxidium pseudocuneiforme n. sp. for this myxozoan from common carp. Its mature myxospores are ellipsoidal and asymmetric with pointed ends in valvular view, arc‐shaped or fusiform in sutural view. The pyriform polar capsules are equal in size, and polar filament with 5–6 coils. This study highlights that molecular characteristics and host specificity are indispensable for myxozoan species identification when presented with the taxonomic dilemma of whether we are observing one species that exhibits slight morphological differences or multiple, but similar, species in different hosts.

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“…Valenciennes; Hechuan District, Chongqing, China Liu et al (2019a) M. hardevi Gupta et Kaur, 2020 P. round, in gills, 0.4-0.5; SP rounded, bowl-shaped in frontal view, broad and flattened anterior end and rounded posterior one, 8.9 × 6.2, prominent thickening between polar capsules; PC unequal, round to bottle-shaped, the larger 3.3 × 2.7, the smaller 1.3 × 1.3, NC 5-6 and 2-3 in the larger and smaller, respectively; IP absent. Fig.…”

Section: Hypophthalmichthys Molitrixmentioning

confidence: 99%

Synopsis of the species of Myxobolus (Cnidaria, Myxozoa, Myxosporea) described between 2014 and 2020

et al. 2021

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A synopsis of the species of Myxobolus Bütschli, 1882 (Cnidaria, Myxosporea, Myxobolidae) described from 2014 up till now is presented. It includes 122 nominal species described all over the world. For each of the species, the most relevant morphological and morphometric data, as well as data are provided related to the location in the host, type host and type locality. The GenBank accession numbers are provided whenever possible, and the spores were redrawn based on the original descriptions. The bibliography includes all the papers containing the species descriptions.

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Morphological and molecular characterization of a new species Myxobolus gutturocola n. sp. (Myxozoa: Myxobolidae) from the throat of Hypophthalmichthys molitrix in China

Cited by 9 publications

References 43 publications

Morphological description and molecular identification ofMyxobolus dajiangensisn. sp. (Myxozoa: Myxobolidae) from the gill ofCyprinus carpioin southwest China

Morphological description and molecular identification ofMyxobolus dajiangensisn. sp. (Myxozoa: Myxobolidae) from the gill ofCyprinus carpioin southwest China

Description of Myxidiumpseudocuneiforme n. sp. (Myxosporea: Myxidiidae) from Cyprinuscarpio in China, with the resolution on a taxonomic dilemma of Myxidiumcuneiforme

Synopsis of the species of Myxobolus (Cnidaria, Myxozoa, Myxosporea) described between 2014 and 2020

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