Morphological and Molecular Characterization of the Thelohania solenopsae Complex (Microsporidia: Thelohaniidae)

Moser, Bettina A.; Becnel, James J.; Williams, David F.

doi:10.1006/jipa.1999.4895

Cited by 15 publications

(24 citation statements)

References 20 publications

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“…3d, e, f, l) in thin sections measured 2?8±0?69 mm (n=21)61?6±0?42 mm (n=26). Their ultrastructure also was consistent with earlier descriptions (Knell et al, 1977;Moser et al, 2000). They are diplokaryotic and develop in direct contact with host cell cytoplasm, their polar filament arranged in one row of 12-13 isofilar coils.…”

Section: Spore Ultrastructuresupporting

confidence: 89%

“…3a-c, i) were found in all of the examined tissues; their appearance and internal structure were similar to those described previously (Knell et al, 1977;Moser et al, 2000). They preserved their integrity after fixation and embedding much better than other spore types.…”

Section: Spore Ultrastructuresupporting

confidence: 86%

“…Uninuclear octospores developing inside a sporophorous vesicle, eight spores in each, and Nosema-like free diplokaryotic spores (Fig. 2a, b, c, f, g, h) were similar to those described previously (Knell et al, 1977;Moser, 1995;Moser et al, 2000). Nosema-like spores were the only spore type that easily discharged their polar filament on fresh smears.…”

Section: Light Microscopysupporting

confidence: 83%

“…The fine morphology of octospores and Nosema-like spores was identical between the current study and those of T. solenopsae isolates from Brazil (Knell et al, 1977;Moser, 1995;Moser et al, 2000), Argentina (Moser, 1995;Moser et al, 2000) and Florida (infecting fire ants in Florida or Louisiana) (Moser, 1995;Moser et al, 2000;Sokolova & Fuxa, 2001). The current ultrastructural analysis confirmed that macrospores, observed also by Knell et al (1977), are teratospores, resulting from abnormal development of octospores.…”

Section: Other Spore Typessupporting

confidence: 82%

“…SSU rDNA sequences of spores from Rosepine (Louisiana isolate) did not differ from those from Clinton (Florida isolate). In all cases, the sequences of regions amplified by the 1TSSf-2TSASr and TS841f-TS1059r primer pairs were highly homologous between octospores and megaspores, because BLAST alignment analysis (Table 1) indicated that both spore types closely matched the T. solenopsae SSU rDNA sequence (Moser et al, 1998(Moser et al, , 2000, accession numbers AF134205, AF031537 and AF031538.…”

Section: Pcr-based Analysis Of Spores Isolated From Smears By Lpc Micmentioning

confidence: 97%

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Spore morphotypes of Thelohania solenopsae (microsporidia) described microscopically and confirmed by PCR of individual spores microdissected from smears by position ablative laser microbeam microscopy

et al. 2004

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Development of Thelohania solenopsae, a parasite of the red imported fire ant (Solenopsis invicta), until recently was thought to include formation of two types of spores: unicellular meiospores, maturing inside sporophorous vesicles in sets of eight (octospores); and Nosema-like binuclear free spores. Megaspores, discovered in 2001, develop primarily in alates and are morphologically distinct from the two previously known types of spores. The role of megaspores in the T. solenopsae life cycle, as well as their existence, has been questioned. The current research includes light and electron microscopic descriptions of the three major spore morphotypes characteristic of T. solenopsae development. In addition, individual octospores and megaspores were isolated into groups of 8-20 from methanol-fixed and Calcofluor-stained smears of the infected ants for subsequent PCR analysis by the laser pressure catapulting function of a position ablative laser microbeam microscope, a technique applied for the first time to research of microsporidia. The PCR-amplified SSU rDNA nucleotide sequences from octospores and megaspores were identical. This, along with the consistency with which megaspores are detected in infected ants, demonstrates that megaspores are integral to the life cycle of T. solenopsae.

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Section: Spore Ultrastructuresupporting

confidence: 89%

Section: Spore Ultrastructuresupporting

confidence: 86%