Morphological and molecular characterization of Tulasnella spp. fungi isolated from the roots of Epidendrum secundum, a widespread Brazilian orchid

Pereira, Marlon Corrêa; Coelho, Irene da Silva; Valadares, Rafael Borges da Silva; Oliveira, Sabrina Feliciano; Bocayuva, Melissa Faust; Pereira, Olinto Liparini; Araújo, Elza Fernandes de; Kasuya, Maria Catarina Megumi

doi:10.1007/s13199-014-0276-0

Cited by 15 publications

(5 citation statements)

References 32 publications

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“…The formation of asexual resistant propagules (monilioid cells), dolipore septum, constriction at branching point of the hypha, bi-or multinucleate vegetative and monilioid cells are the common anamorphic features of Rhizoctonialike fungi widely reported as orchid mycorrhizal fungi (Currah et al, 1997;Sneh et al, 1991;Otero et al, 2002;Shan et al, 2002). Nuclear number per cell varies from uni-, bi-to multinucleate in different orchid mycorrhizal fungi and even in different strains of the same species (Sneh et al, 1991;Otero et al, 2002;Pereira et al, 2014). This framework adopted by several researchers for identifying orchid mycorrhizal fungi offers a taxonomically correct and justified approach to define taxa (Shan et al, 2002).…”

Section: Morphological Characterization Of Fungal Endophytementioning

confidence: 99%

Morpho-molecular characterization of Ceratobasidium sp.: A mycorrhizal fungi isolated from a rare epiphytic orchid Gastrochilus calceolaris (J. E. Sm.) D. Don

Hossain¹

2019

Bangladesh J. Plant Taxon

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A mycorrhizal fungus, Ceratobasidium sp. GC (NCBI Gene Bank Accession no GQ369961) associated with the roots of an epiphytic orchid Gastrochilus calceolaris was investigated by cultural morphology, microscopic features and molecular analysis of Internal Transcribed Spacer (ITS) regions sequences of nuclear ribosomal DNA. The colony appearance of the fungal endophyte was fluffy growth pattern and the colour of the young colony was milky white on both surfaces that turned in to brown at maturity on the upper surface and deep brown on reverse surface. The microscopic features of the fungus i.e. hyphal diameter, multi-nucleate vegetative cells, right angle branching pattern with slight constriction at branching point and a dolipore septum near the branching point were observed. All the characters corroborated the identity with anamorphic Rhizoctonia like fungi. The ITS regions sequences of nrDNA and phylogenetic analysis based on the neighbor-joining method showed clustered with Rhizoctonia like fungi, and the maximum identity (98.28%) with Ceratobasidium RR and Ceratobasidium FPUB isolated from Rhynchostylis retusa and Dactylorrhiza hetagera, respectively. Thus, the ITS of nrDNA sequences validated the morphological data. This is the first report of orchid mycorrhizal fungi from Bangladesh.

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Section: Morphological Characterization Of Fungal Endophytementioning

confidence: 99%

Morpho-molecular characterization of Ceratobasidium sp.: A mycorrhizal fungi isolated from a rare epiphytic orchid Gastrochilus calceolaris (J. E. Sm.) D. Don

Hossain¹

2019

Bangladesh J. Plant Taxon

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“…Recent studies have researched tropical orchids in South and Central America (Otero et al 2002;2004;Pereira et al 2005;Otero et al 2007;Otero & Bayman 2009;Pereira et al 2009;Valadares et al 2012;Oliveira et al 2014;Pereira et al 2014), but only one demonstrated ITS rDNA sequences from mycorrhizal fungi in Colombia (Mosquera et al 2010;. In Ecuador, Suarez et al (2006) isolated mycorrhizal fungi belonging to the genera Tulasnella and Sebacina.…”

Section: Introductionmentioning

confidence: 99%

The epiphytic orchids Ionopsis utricularioides and Psygmorchis pusilla associate with different Ceratobasidium lineages at Valle del Cauca, Colombia

et al. 2015

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In Orchidaceae, association with symbiotic fungi is required for seed germination and seedling development, thereby being the main energy source during the first steps of germination. Colombia is one of the countries with the greatest biodiversity of orchids, with an estimated 3,200 species, but few studies on orchid mycorrhiza have been conducted. In our study, we isolated and sequenced the internal transcribed spacer rDNA region of fungi from two co-occurring Colombian epiphytic orchids, I. utricularioides and P. pusilla, both belonging to the subtribe Oncidiinae. All sequences were recognized as belonging to the genus Ceratobasidium, known to be a common orchid mycorrhizal fungus in both tropical and temperate orchids. One sequence was 100% similar to fungi isolated from I. utricularioides in Costa Rica in a previous study. I. utricularioides was confirmed to be a specialist, associating with only one clade of mycorrhizal fungi. However, P. pusilla was shown to be a generalist, associating with three clades. This finding indicates that the variation in mycorrhizal specificity could be an important factor in the co-existence of orchids. The high affinity between the subtribe Oncidiinae and Ceratobasidium was also reinforced.

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“…The genus Tulasnella is often observed as orchid mycorrhizal fungi in temperate and tropical regions 12,18,19 , and several isolates have been reported to increase seed germination and seedling growth [20][21][22][23][24][25] . Identification of mycorrhizal fungi in South American orchids, mostly conducted in Brazil, has often revealed Tulasnella symbionts: Tulasnella species were isolated from Epidendrum secundum 26,27 , Epidendrum dendrobioides and Sophronits milleri 28 , Oeceoclades maculata, Epidendrum rigidum and Polystachya concreta 29 , E. rigidum and P. concreta 30 . Yet little is known about Tulasnella in the hotspot of biodiversity of the Brazilian Atlantic Forest.…”

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Diversity of mycorrhizal Tulasnella associated with epiphytic and rupicolous orchids from the Brazilian Atlantic Forest, including four new species

Freitas

Silva

et al. 2020

Sci Rep

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the genus Tulasnella often forms mycorrhizas with orchids and has worldwide distribution. Species of this genus are associated with a wide range of orchids, including endangered hosts. initially, species identification relied mostly on morphological features and few cultures were preserved for later phylogenetic comparisons. In this study, a total of 50 Tulasnella isolates were collected from their natural sites in Minas Gerais, Brazil, cultured, and subjected to a phylogenetic analysis based on alignments of sequences of the internal transcribed spacer (itS) of the nuclear ribosomal DnA. our results, based on phylogeny, integrated with nucleotide divergence and morphology, revealed the diversity of isolated Tulasnella species, which included four new species, namely, Tulasnella brigadeiroensis, Tulasnella hadrolaeliae, Tulasnella orchidis and Tulasnella zygopetali. the conservation of these species is important due to their association with endangered orchid hosts and endemic features in the Brazilian Atlantic forest.

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Morphological and molecular characterization of Tulasnella spp. fungi isolated from the roots of Epidendrum secundum, a widespread Brazilian orchid

Cited by 15 publications

References 32 publications

Morpho-molecular characterization of <i>Ceratobasidium</i> sp.: A mycorrhizal fungi isolated from a rare epiphytic orchid <i>Gastrochilus calceolaris</i> (J. E. Sm.) D. Don

Morpho-molecular characterization of <i>Ceratobasidium</i> sp.: A mycorrhizal fungi isolated from a rare epiphytic orchid <i>Gastrochilus calceolaris</i> (J. E. Sm.) D. Don

The epiphytic orchids Ionopsis utricularioides and Psygmorchis pusilla associate with different Ceratobasidium lineages at Valle del Cauca, Colombia

Diversity of mycorrhizal Tulasnella associated with epiphytic and rupicolous orchids from the Brazilian Atlantic Forest, including four new species

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