Morphological and Molecular Characterization of <i>Oscheius saproxylicus</i> sp. n. (Rhabditida, Rhabditidae) From Decaying Wood in Spain, With New Insights into the Phylogeny of the Genus and a Revision of its Taxonomy

Abolafia, Joaquín; Peña‐Santiago, Reyes

doi:10.21307/jofnem-2019-053

Cited by 8 publications

(17 citation statements)

References 58 publications

(92 reference statements)

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“…Compared to the other LSU sequence assigned to O. myriophilus (MK418537), the newly generated LSU sequences had remarkable differences (2 gaps and 13 indels), thus the identity of this sequence needs further validations. The presently resolved SSU phylogeny, is similar to the resolved tree by Abolafia and Peña-Santiago (2019) , corroborating members of the Insectivorus-group and Dolichura-group form monophyletic groups, both of which forming the monophyletic genus Oscheius (based on currently available data). The similar pattern was also observed in LSU phylogeny.…”

Section: Discussionsupporting

confidence: 73%

“…It is mainly characterized by a short buccal tube about as long as wide, and absence of median pharyngeal swelling. Abolafia and Peña-Santiago (2019) presented an excellent revision on taxonomy and phylogeny of the genus, discussed on its valid subgenera and intragenus grouping of the valid species. After the SSU and LSU phylogenetic trees in their study, the genus seems to be monophyletic based on both markers.…”

Section: Discussionmentioning

confidence: 99%

“…The maximal number of sequences of Oscheius spp. ( Abolafia and Peña-Santiago, 2019 ) and sequences from several other classic rhabditid genera were selected for reconstructing of the phylogenetic trees. The alignment of SSU sequences was done using the Q-INS‐i algorithm of online version of MAFFT version 7 ( http://mafft.cbrc.jp/ alignment/server/) ( Katoh and Standley, 2013 ).…”

Section: Methodsmentioning

confidence: 99%

“…Tracer v1.5 software ( Rambaut and Drummond, 2009 ) was used to visualize the results of each run in order to check the effective sample size of each parameter. The outgroup sequences in both phylogenies were selected according to Abolafia and Peña-Santiago (2019) . The output file of the used phylogenetic program was visualized using Dendroscope V.3.2.8 ( Huson and Scornavacca, 2012 ) and the tree was redrawin in CorelDRAW software version 2017.…”

Section: Methodsmentioning

confidence: 99%

“…It belongs to the family Rhabditidae, has two intragenus groupings, namely Dolichura and Insectivora ( Sudhaus, 1976 ), the first group being characterized by having a peloderan bursa, thin tubular spicule tips; and the second group is characterized by a leptoderan or pseudopeloderan bursa, and crochet needle-shaped spicules. Currently, there are 23 species of Insectivora group and 16 species of Dolichura group ( Abolafia and Peña-Santiago, 2019 ). Some species of Oscheius are necromenic ( Sudhaus and Schulte,1989 ; Dillman et al, 2012 ).…”

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confidence: 99%

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First record of Oscheius myriophilus (Poinar, 1986) (Rhabditida: Rhabditidae) from Iran; and its efficacy against two economic forest trees pests, Cydalima perspectalis (Walker, 1859) (Lepidoptera: Crambidae) and Hyphantria cunea (Drury, 1773) (Lepidoptera: Erebidae) in laboratory condition

Ghavamabad

Talebi

Mehrabadi

et al. 2021

Journal of Nematology

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The box tree moth (BTM), Cydalima perspectalis and the fall webworm (FWW), Hyphantria cunea are two invasive pests of forest trees that have been recorded from Hyracinan forests in north Iran for the first time in 2016 and 2002, respectively. In a search for tentative native entomopathogenic nematode species (EPNs) with potential biocontrol ability against lepidopteran pests of forest trees in north Iran, Oscheius myriophilus was isolated by soil-baiting method from forests of Amlash in the east of Gilan province. The Iranian isolate of this species is characterized by 870–1,247 μm long hermaphrodites having 17–20 μm long stoma, vulva at 45.5–53.1% of body length, tail 90–126 μm long, common males with 38–49 μm long spicules and 583–791 μm long dauer larvae. Compared to the type and other populations, no remarkable differences were observed for this population. The phylogenetic affinities of this isolate with other rhabditid nematodes were studied using partial sequences of small, and the D2-D3 expansion segments of the large subunit ribosomal DNA (SSU and D2-D3 LSU rDNA). This is the first record of the species from Iran. The pathogenicity of Iranian isolate of O. myriophilus was evaluated on the larvae of two important aforementioned forest trees pests, BTM and FWW under laboratory conditions. The results indicated that the suspension of 500 infective juveniles per ml (IJs/ml) of the nematode was the most effective treatment on fifth instar larvae of BTM and FWW, causing 100 and 95% mortality after 48 h, respectively. The lethal concentration 50 (LC50) values of the nematode were 74.5, 152.7, 99.9, and 197.3 IJs/ml on fifth and fourth instar larvae of BTM, and fifth and fourth instar larvae of FWW, respectively, after 48 h at 25°C and 60% relative humidity. Together, present results corroborated the efficacy of the Iranian isolate of Oscheius myriophilus for biocontrolling of BTM and FWW in laboratory conditions.

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Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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First record of Oscheius myriophilus (Poinar, 1986) (Rhabditida: Rhabditidae) from Iran; and its efficacy against two economic forest trees pests, Cydalima perspectalis (Walker, 1859) (Lepidoptera: Crambidae) and Hyphantria cunea (Drury, 1773) (Lepidoptera: Erebidae) in laboratory condition

Ghavamabad

Talebi

Mehrabadi

et al. 2021

Journal of Nematology

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Redescription and synonymization of Oscheius citri Tabassum, Shahina, Nasira and Erum, 2016 (Rhabditida, Rhabditidae) from India and its taxonomical consequences

et al. 2021

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A population of a nematode species belonging to the genus Oscheius was isolated in western Uttar Pradesh, India. Morphological and morphometrical studies on this species showed its high similarity with six species described previously from Pakistan (Oscheius citri, O. cobbi, O. cynodonti, O. esculentus, O. punctatus and O. sacchari). The molecular analysis of the ITS1-5.8S-ITS2 rDNA sequences of the Indian population and the six species described from Pakistan showed that all the sequences are almost identical. Thus, based on morphological and molecular characteristics, all of the six above-mentioned Pakistani species and Indian strain do not differ from each other, hence can be considered synonyms. The correct name for this taxon is the first described species O. citri. Additionally, the phylogenetic analysis of the 18S rDNA and the 28S rDNA sequences showed that Oscheius citri is sister to the clade formed by O. chongmingensis and O. rugaoensis from China. The high similarity of morphological and morphometric characteristics of O. citri and other species, O. maqbooli, O. nadarajani, O. niazii, O. shamimi and O. siddiqii, suggest their conspecificity; however, lack of molecular data for these species does not allow this hypothesis to be tested.

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Description ofOscheius cyrusn. sp. (Nematoda: Rhabditidae) as new entomopathogenic nematode from Iran

et al. 2022

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A new species of the genus Oscheius, Oscheius cyrus n. sp., collected in the moist soils taken from forest heights in the north of Iran, is recorded. A comprehensive description, comprising molecular (internal transcribed spacer (ITS), 18S, and 28S rDNA genes) information, morphometrics data, light microscope and scanning electron microscope images, is supplied. The species resembles Oscheius myriophilus. However, the highest ranges for female body length, female tail, infective juvenile tail length, median bulb, absence of epiptygma and lateral field incisures number vary. The new species was distinguished from Oscheius insectivorus by the general lip region. The male was not found. Molecular analysis showed that the new species has the most similarity to O. myriophilus both in the ITS and 18S regions. Morphological and molecular data confirmed its belonging to the Insectivora-group. Furthermore, the species of Ochrobactrum pseudogrignonense was reported as a dominant associated bacterium of the new Oscheius species. Finally, the mortality of the host after seven days varied from 20% to 82.5%, depending on nematodes’ concentration.

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Morphological and Molecular Characterization of Oscheius saproxylicus sp. n. (Rhabditida, Rhabditidae) From Decaying Wood in Spain, With New Insights into the Phylogeny of the Genus and a Revision of its Taxonomy

Cited by 8 publications

References 58 publications

First record of Oscheius myriophilus (Poinar, 1986) (Rhabditida: Rhabditidae) from Iran; and its efficacy against two economic forest trees pests, Cydalima perspectalis (Walker, 1859) (Lepidoptera: Crambidae) and Hyphantria cunea (Drury, 1773) (Lepidoptera: Erebidae) in laboratory condition

First record of Oscheius myriophilus (Poinar, 1986) (Rhabditida: Rhabditidae) from Iran; and its efficacy against two economic forest trees pests, Cydalima perspectalis (Walker, 1859) (Lepidoptera: Crambidae) and Hyphantria cunea (Drury, 1773) (Lepidoptera: Erebidae) in laboratory condition

Redescription and synonymization of Oscheius citri Tabassum, Shahina, Nasira and Erum, 2016 (Rhabditida, Rhabditidae) from India and its taxonomical consequences

Description ofOscheius cyrusn. sp. (Nematoda: Rhabditidae) as new entomopathogenic nematode from Iran

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