Morphological and molecular characterization of Sclerotium rolfsii associated with stem rot disease of groundnut (Arachis hypogaea L.)

Hawaladar, Sachin; Nandan, M.; Vinaykumar, H. D.; Hadimani, Rajanna H.; Hiremath, Shridhar; Venkataravanappa, V.; Basha, C. R. J.; Reddy, C. N. Lakshminarayana

doi:10.1007/s42360-021-00419-y

Cited by 4 publications

(2 citation statements)

References 27 publications

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“…phylogenetic analysis the current isolates were shown to belong to two separate clades of Athelia rolfsii. SrD1 was assigned to clade 1, while the remaining nine isolates (SrB1, SrB2, SrC1, SrC2, SrD2, SrP1, SrP2, SrY1 and SrY2) were assigned to clade 2 (Hawaldar et al, 2021). Ten isolates of S. rolfsii collected from groundnut grown areas were investigated for their genetic variability study through ITS amplification that produced rDNA amplicon size of 650-700 bp which confirmed that all isolates belong to Sclerotium rolfsii.…”

Section: Resultsmentioning

confidence: 70%

Diversity Studies of Sclerotium rolfsii Sacc. Isolates Associated with Collar Rot of Soybean Through Molecular and Biochemical Approaches

Hiremath,

Jahagirdar,

Krishnaraj

et al. 2024

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Background: Sclerotium rolfsii Sacc. is one of the potential soil-borne plant pathogens due to its significant loss in terms of yield and quality in several crop plants. In soybean the pathogen is associated with collar rot disease. Methods: Sixteen samples which were showing collar rot symptoms were collected from different soybean growing regions of the country and were subjected for isolation and purification. Molecular detection was carried out using ITS1/4 primers followed by phylogenetic analysis was performed using the MEGA 11 bioinformatics tool. Further, the biochemical test was made for oxalic acid production to determine the variability across the isolate. Result: DNA was amplified for ITS rDNA region at 650-700 bp in all the sixteen isolates. BLAST detection of nucleotide sequence of sixteen isolates framed mainly into two distinct phylogenetic clusters. Cluster B consisted of two isolates (MPSe 1 and MHSa 3) whereas, Cluster A contains the remaining fourteen isolates. There was a variation with respect to oxalic acid production among the isolates which was in the rage of 1.15 to 2.23 mg/ml.

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Section: Resultsmentioning

confidence: 70%

Diversity Studies of Sclerotium rolfsii Sacc. Isolates Associated with Collar Rot of Soybean Through Molecular and Biochemical Approaches

Hiremath,

Jahagirdar,

Krishnaraj

et al. 2024

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“…Currently, molecular identification may be performed by sequencing the Internal Transcribed Spacer (ITS). ITS sequencing has been widely utilized in fungal molecular characterization at the species level (Schoch et al 2012;Xu et al 2015;Hawaladar et al 2022). The purpose of this study was to identify the fungal species causing black collar rot disease of groundnut based on morphological characterization, and ITS sequence analysis.…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of Aspergillus niger causing collar rot of groundnut (Arachis hypogaea)

Nguyen

et al. 2023

Biodiversitas

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Abstract. Nguyen XH, Nguyen TMN, Nguyen DH, Nguyen QC, Cao TT, Pham TTH, Nguyen TTT. 2023. Identification and characterization of Aspergillus niger causing collar rot of groundnut (Arachis hypogaea). Biodiversitas 24: 2556-2562. Groundnut (Arachis hypogaea L.) is an economically important legume crop in Vietnam and many other countries worldwide. Groundnut cultivation is affected by many biotic and abiotic stresses. Among biotic stresses, groundnut is attacked by many fungal, bacterial and viral pathogens. The most harmful fungal diseases are rust, stem rot, collar rot and other soil borne diseases. Collar rot caused by Aspergillus niger is one of the most important disease of groundnut extensive in Vietnam and worldwide. In this study, symptomatic infected plant samples were collected from nine different locations in Quang Tri and Thua Thien Hue provinces, Vietnam Isolated fungal species were identified on the basis of morphological characterization, and molecular level. The pathogenicity of fungal species was confirmed on the basis of Koch's postulates. The result showed that a total of nine fungal strains were isolated from infected groundnut samples. Morphological analysis results showed that all isolates exhibited A. niger characteristics, including black colony, biseriate conidia, hyaline conidiophores and sterigmata. Pathogenicity test showed that all nine A. niger isolates caused collar rot disease in groundnuts. Further molecular identification based on ITS1-4 region nucleotides comparison indicated that selected two isolates, namely QT1 and H17 belonged to A. niger. Further research should be done to find antagonistic microorganisms isolates for the bio-control of A. niger.

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Fungal Root Rots of Sugar Beets: A Review of Common Causal Agents and Management Strategies

et al. 2023

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Morphological and molecular characterization of Sclerotium rolfsii associated with stem rot disease of groundnut (Arachis hypogaea L.)

Cited by 4 publications

References 27 publications

Diversity Studies of Sclerotium rolfsii Sacc. Isolates Associated with Collar Rot of Soybean Through Molecular and Biochemical Approaches

Diversity Studies of Sclerotium rolfsii Sacc. Isolates Associated with Collar Rot of Soybean Through Molecular and Biochemical Approaches

Identification and characterization of Aspergillus niger causing collar rot of groundnut (Arachis hypogaea)

Fungal Root Rots of Sugar Beets: A Review of Common Causal Agents and Management Strategies

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