Morphological and molecular characterization of a new isolate of entomopathogenic nematode, Steinernema feltiae (Filipjev) (Rhabditida: Steinernematidae) from the Arasbaran forests, Iran

Nikdel, M; Niknam, Gholamreza

doi:10.1016/j.japb.2015.04.008

Cited by 17 publications

(9 citation statements)

References 10 publications

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“…Previously, S. feltiae was recovered from the coast of the Black Sea and Ankara (Özer et al 1995;Susurluk et al, 2002;Hazir et al, 2003a) and in some cases, it was the most common species in those area (Hazir et al 2003b; Eivazian Kary et al, 2009;Yuksel and Canhilal, 2019). Similarly, S. feltiae was reported from several provinces such as Tehran, Mazandaran, East Azerbaijan, Ardabil, Kurdistan in Iran (Tanha Ma'a et al, 2006; Eivazian Kary et al, 2009;Karimi et al, 2009;Nikdel and Niknam, 2015).…”

Section: Discussionmentioning

confidence: 84%

Steinernema Feltiae- Xenorhabdus Bovienii: More Information about this Bactohelminthic Complex from Iran

Kuhestani

Karimi

Makhdomi

2021

Preprint

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Background: Entomopathogenic nematodes (EPNs) of the families Steinernematidae and Heterorhabditidae that are symbiotically associated with Xenorhabdus and Photorhabdus bacteria are one of the effective biological control agents of insect pests. Native isolates can probably be more efficacious to control insect pests than exotic ones due to their adaptability to indigenous environmental conditions. Results: In this study, Steinernema feltiae isolate FUM221 was recovered from soil samples collected from the Fandoghloo pasture, Ardabil province, Iran. Morphological investigations of the first and second-generation adults, infective juveniles, and molecular characterizations were given based on ITS and 18S rDNA genes. Besides, molecular analysis based on the 16S rRNA region and phenetic data recognized Xenorhabdus bovienii as its symbiont bacterium. The scanning electron microscopy (SEM) images verified the identification of this isolate.Conclusion: The molecular characterization using two loci and phylogenetic analyses provided more evidence for the classification of this steinernematid and its difference of the same species from other countries. Moreover, molecular and phenetic characterizations of its symbiotic bacterium were provided with low variations compared to other isolates. Herein, the comprehensive taxonomic data of this steinernematid from Iran is presented.

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Section: Discussionmentioning

confidence: 84%

Steinernema Feltiae- Xenorhabdus Bovienii: More Information about this Bactohelminthic Complex from Iran

Kuhestani

Karimi

Makhdomi

2021

Preprint

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“…Morphometric characteristics of S. feltiae showed a great intraspecific variability in mean values and ranges between isolates from different geographical origins [ 35 – 41 ]. In Chile, Edgington et al [ 9 ] collected several isolates of this species from different zones (3, 4 and 6), and, based on ITS region, they were separated into two subgroups (I and II).…”

Section: Discussionmentioning

confidence: 99%

Morphological, molecular and ecological characterization of a native isolate of Steinernema feltiae (Rhabditida: Steinernematidae) from southern Chile

et al. 2021

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Background Steinernema feltiae is an entomopathogenic nematode used in biological control programs with a global distribution. Populations of this species show phenotypic plasticity derived from local adaptation and vary in different traits, such as location and host penetration. The aim of this work was to describe a Chilean isolate of this nematode species, using integrative approaches. Methods Nematode morphological and morphometric studies were conducted along with molecular analysis of nuclear genes. The symbiotic bacterium was also identified by sequencing the 16S rRNA gene. Some ecological characteristics were described, including the temperature requirements for the nematode life cycle and the effect of soil water content for optimal reproduction. Results Morphometric characterization revealed a large intra-specific variability. The isolate identity was also corroborated with the analysis of nuclear genes. Based on the 16S gene, its symbiont bacteria, Xenorhabdus bovienii, was identified. The lowest, optimal and highest temperatures found to limit the infestation and reproduction on Galleria mellonella were 10, 20 and 30 °C, respectively; the emergence from the host larvae occurred approximately 10 days after inoculation. Differences were observed in offspring, and 120 infective juveniles (IJ)/larva was the most prolific dose at 20 °C. The soil water content did not affect the number of IJ invaders, penetration efficacy and IJ emergence time or offspring per larva, but it caused a delay in achieving full mortality at the permanent wilting point with respect to saturation and field capacity. Conclusions For the first time, a Chilean isolate of S. feltiae is described in detail considering morphological, molecular and ecological aspects. The isolate was shown to be efficient in soil containing water, with optimal temperatures ranging from 15 to 25 °C for host infestation and production of an abundant offspring; these characteristics would allow its potential use as control agents in a wide geographical area of the country.

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“…Despite the fact that S. feltiae had already been isolated from Chilean soils (9), this is the rst time that an isolate has been described in detail, taking into account morphological, molecular and ecological characteristics. Morphometric characteristics from the different life stages are analyzed, showing great intraspeci c variability in the averages and ranks between isolates from different geographical origins (35,36,37,38,39,40,41). In Chile, Edgington et al (9) collected several S. feltiae isolates from 6 zones and based on ITS region, classi ed the isolates into two subgroups (I and II) based on the ITS region.…”

Section: Discussionmentioning

confidence: 99%

Morphological, Molecular and Ecological Characterization of a Native Isolate of Steinernema Feltiae (Rhabditida: Steinernematidae) From Southern Chile

Flores

Alvarado

Lankin

et al. 2020

Preprint

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Background: Steinernema feltiae is an entomopathogenic nematode used in biological control programs with a global distribution. It has been shown that populations of S. feltiae may have phenotypic plasticity derived from local adaptation and may vary in different traits, such as location and penetration of the host. This is the first time that a Chilean isolate has been described in detail, taking into account morphological, molecular and ecological characteristics. Methods: S. fealtiae was detected a few years ago in southern regions of Chile in the town Lican Ray and was morphologically and morphometrically described, along with a molecular description based on the ITS and D2-D3 regions. Some ecological characteristics were determined, including the temperature requirements for completion of its life cycle and the effect of three water levels in soil for optimal reproduction. Results: Morphometric characteristics of different life stages show large intraspecific variability in averages and ranks compared with isolates from different geographical origins. The molecular data also show intraspecific variability with respect to other isolates. The lower, optimal and higher temperatures found to limit the infestation and reproduction of Galleria mellonella were 10, 20 and 30 °C respectively, and emergence from the host larvae occurred approximately ten days after inoculation. Differences were observed in offspring emerging from the host, and the 120 infective juveniles (IJ)/larvae dose was the most prolific at 20 °C. The water content of the soil did not affect the number of infective juvenile invaders, penetration efficacy (%), and time of emergence of the IJ or offspring per larvae, but it caused a delay in achieving full mortality at the permanent wilting point with respect to saturation and field capacity.Conclusions: Data obtained in this study are valuable since they outline some environmental requirements of this strain to perform optimally in the event of being used as a soil pest bioantagonist.

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Morphological and molecular characterization of a new isolate of entomopathogenic nematode, Steinernema feltiae (Filipjev) (Rhabditida: Steinernematidae) from the Arasbaran forests, Iran

Cited by 17 publications

References 10 publications

Steinernema Feltiae- Xenorhabdus Bovienii: More Information about this Bactohelminthic Complex from Iran

Steinernema Feltiae- Xenorhabdus Bovienii: More Information about this Bactohelminthic Complex from Iran

Morphological, molecular and ecological characterization of a native isolate of Steinernema feltiae (Rhabditida: Steinernematidae) from southern Chile

Morphological, Molecular and Ecological Characterization of a Native Isolate of Steinernema Feltiae (Rhabditida: Steinernematidae) From Southern Chile

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