Morphological and molecular characterisation of Aporcelaimellus nigeriensis sp. n. (Dorylaimida: Aporcelaimidae), a remarkable dorylaim from Nigeria

Abstract

Summary A new species of Aporcelaimellus, collected in a watermelon field in Nigeria, is described, including its morphological and molecular (D2-D3 28S-rDNA, 18r-DNA) characterisation. Aporcelaimellus nigeriensis sp. n. is distinguishable by its 2.76-3.55 mm length, very coarse ventral body pores, lip region offset by deep constriction and 24-27 μm broad odontostyle 30-36 μm long at its dorsal and 28-31 μm at its ventral side, neck 648-779 μm long, pharyngeal expansion occupying 54-60% of total neck length, uterus 300-473 μm or 2.1-3.2 body diam. long and tripartite, V = 49-54, tail short and convex conoid (27-41 μm, c = 72-115, c′ = 0.5-0.7), spicules 108-137 μm long, and 9-10 spaced ventromedian supplements with hiatus. LSU analysis revealed a close relationship of A. nigeriensis sp. n. with other Aporcelaimellus species and questioned, once more, the monophyly of Aporcelaimidae. SSU phylogenetic tree was not able to resolve the relationship between the new species and other closely related species.

Morphological and molecular characterization of Metaxonchium magnum sp. n. from Iran (Nematoda, Dorylaimida, Belondiridae), with new insights into the phylogeny of the genus

“…PCR reactions were carried out in a DNA thermal cycler (Hybaid, Ashford, Middlesex, UK). The PCR programme was followed as reported by Rashidifard et al (2020). The quality of PCR were checked by electrophoresis of 4 μl of the PCR reactions in 1% agarose gel containing SYBR Green I.…”

Morphological and molecular characterization of Aporcelinus abeokutaensis sp. n. (Dorylaimida, Aporcelaimidae) from Nigeria

“…The PCR program was followed as reported by Rashidifard et al . (2020). To check the quality of DNA, 4 μl of PCR product was loaded on 1.5% agarose gel and the band was stained using GelRed before the visualization under an ultraviolet transilluminator.…”

Morphological and molecular characterization ofAporcella feminasp. n. (Dorylaimida, Aporcelaimidae) from Nigeria

“…The polymerase chain reaction (PCR) amplification process was followed as reported by Rashidifard et al . (2020). Four microliters of PCR products were loaded on 1% agarose gel and visualized by an ultraviolet transilluminator to check the quality of DNA.…”

“…DNA amplification was carried out on a Vacutec thermocycler (www.vacutec.co.za) using the primers: small subunit (SSU) F04 (5 ′ -GCTTGTCTCAAAGATTAAGCC-3 ′ ), and SSU R26 (5 ′ -CATTCTTGGCAAATGCTTTCG-3 ′ ) (Blaxter et al, 1998) for SSU ribosomal DNA (rDNA), as well as D2A (5 ′ -ACAAGTA CCGTGAGGGAAAGTTG-3 ′ ) and D3B (5 ′ -TCGGAAGGAA CCAGCTACTA-3 ′ ) (Subbotin et al, 2006) for D2-D3 large subunit (LSU) rDNA. The polymerase chain reaction (PCR) amplification process was followed as reported by Rashidifard et al (2020). Four microliters of PCR products were loaded on 1% agarose gel and visualized by an ultraviolet transilluminator to check the quality of DNA.…”

“…The new SSU and LSU sequences obtained for the new species were compared to other available sequences in the GenBank using the BLASTN search tool. One data set was prepared for each of the SSU and LSU regions and taxa selection was carried out according to Rashidifard et al (2020). The available sequences as well as outgroups in the SSU data set were aligned using the MUSCLE alignment tool (Edgar, 2004) in Geneious Prime® 2020.2.3 (www.geneious.com), while the LSU data set was aligned using the E-INS-I algorithm in the MAFF alignment tool (Katoh & Standley, 2013).…”