Morphological and Molecular Analyses of Closely Related Species In the Stygobiontic Genus Niphargus (Amphipoda)

Hartke, Tamara R.; Hohagen, Jennifer; Kleber, Sascha; Hartmann, Rainer; Koenemann, Stefan

doi:10.1651/10-3434.1

Cited by 11 publications

(12 citation statements)

References 15 publications

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“…; Hartke et al . ). Also included were published sequence data of the mitochondrial 12s rRNA region (12S) and the large subunit 18s rRNA (18S) for the taxa covered in the combined data set.…”

Section: Methodsmentioning

confidence: 97%

“…Genetic variation of Niphargus was assessed at two mitochondrial genes, cytochrome oxidase subunit I (COI) and 16s rRNA (16S) and the nuclear small subunit 28s rRNA (28S; for details see Supplementary methods and Table S2). Our analysis combined these new DNA sequence data with all published Niphargus sequence data for 28S, COI and 16S available on GenBank on 1 June 2012 (Lef ebure et al 2006(Lef ebure et al , 2007Fi ser et al 2008;H€ anfling et al 2008;Trontelj et al 2009;Flot 2010;Flot et al 2010;Hartke et al 2011). Also included were published sequence data of the mitochondrial 12s rRNA region (12S) and the large subunit 18s rRNA (18S) for the taxa covered in the combined data set.…”

Section: De Novo Sequencing and Data Sets For Phylogenetic Analysismentioning

confidence: 99%

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The ancientBritons: groundwater fauna survived extreme climate change over tens of millions of years acrossNW Europe

et al. 2014

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Section: Methodsmentioning

confidence: 97%

Section: De Novo Sequencing and Data Sets For Phylogenetic Analysismentioning

confidence: 99%

The ancientBritons: groundwater fauna survived extreme climate change over tens of millions of years acrossNW Europe

et al. 2014

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“…tridentinus Stoch, 1998 (sequenced using the same protocol as in Flot et al 2010; GenBank accession numbers KT878856 and KT878857) and pre-aligned in MAFFT using the FFT-NS-i mode to identify unsuitable sequences (originating from a different 28S region than our target region). After filtering out these sequences, the remaining dataset of 422 sequences from Lefébure et al (2006Lefébure et al ( , 2007, Fišer et al (2008), Trontelj et al (2009), Flot (2010, Flot et al (2010Flot et al ( , 2014, Hartke et al (2011), Trontelj et al (2012, Hekmatara et al (2013), Fišer et al (2013), McInerney et al (2014), Altermatt et al (2014), Ntakis et al (2015) and Esmaeili-Rineh et al (2015) were screened for perfect duplicates using FaBox (Villesen 2007), resulting in a dataset a 260 unique sequences, to which two outgroup sequences from the genera Synurella Wrzesniowski, 1877 and Gammarus Fabricius, 1775 were added as in Flot et al (2014). The sequences were aligned in MAFFT version 7 (Katoh & Standley 2013) using the E-INS-i option and analyzed in FastTree 2 (Price et al 2010) using the GTR model (Lanave et al 1984;Tavaré 1986) with 1000 bootstrap replicates (Felsenstein 1985).…”

Section: Molecular Analysesmentioning

confidence: 99%

Niphargus dancaui sp. nov. (Amphipoda, Niphargidae) – a new species thriving in sulfidic groundwaters in southeastern Romania

Brad

Flot

Sarbu

2015

EJT

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641

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Abstract. Niphargus dancaui sp. nov., previously referred to as Niphargus cf. stygius, was sampled from various groundwater sites in and near the town of Mangalia (SE Romania) and described with Movile Cave (a sulfidic, chemoautotrophically based ecosystem) as type locality. A short comparison with Niphargus stygius specimens from Slovenia was made, together with a morphological analysis of interpopulational variability. Males of N. dancaui sp. nov. were relatively large (17 mm), with long antennae, pereiopods and uropod III. Females were slightly smaller, with shorter antennae, pereiopods and uropod III. Interpopulational variability was noticed in the chaetotaxy of the telson lobes and uropod iii. N. dancaui sp. nov. is morphologically very close to N. lessiniensis and N. tridentinus, two species present in northern Italy, but distinct genetically from them based on 28S rRNA sequences. Instead, the closest relative of N. dancaui sp. nov. sequenced so far for this marker is N. montanarius, which inhabits a sulfidic cave system in central Italy. The work presented here contributes to our knowledge of groundwater crustacean biodiversity in general and of the systematics of the genus Niphargus in particular.

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“…With more than 300 species, Niphargus is the most speciose freshwater amphipod genus in the world [32]. A high level of cryptic diversity within Niphargus has been uncovered by molecular studies [5], [21], [27], [31], [33]. However, there are no molecular studies for the Eastern part of Europe.…”

Section: Introductionmentioning

confidence: 99%

Can Environment Predict Cryptic Diversity? The Case of Niphargus Inhabiting Western Carpathian Groundwater

et al. 2013

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In the last decade, several studies have shown that subterranean aquatic habitats harbor cryptic species with restricted geographic ranges, frequently occurring as isolated populations. Previous studies on aquatic subterranean species have implied that habitat heterogeneity can promote speciation and that speciation events can be predicted from species’ distributions. We tested the prediction that species distributed across different drainage systems and karst sectors comprise sets of distinct species. Amphipods from the genus Niphargus from 11 caves distributed along the Western Carpathians (Romania) were investigated using three independent molecular markers (COI, H3 and 28S). The results showed that: 1) the studied populations belong to eight different species that derive from two phylogenetically unrelated Niphargus clades; 2) narrow endemic species in fact comprise complexes of morphologically similar species that are indistinguishable without using a molecular approach. The concept of monophyly, concordance between mitochondrial and nuclear DNA, and the value of patristic distances were used as species delimitation criteria. The concept of cryptic species is discussed within the framework of the present work and the contribution of these species to regional biodiversity is also addressed.

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Morphological and Molecular Analyses of Closely Related Species In the Stygobiontic Genus Niphargus (Amphipoda)

Cited by 11 publications

References 15 publications

The ancientBritons: groundwater fauna survived extreme climate change over tens of millions of years acrossNW Europe

The ancientBritons: groundwater fauna survived extreme climate change over tens of millions of years acrossNW Europe

Niphargus dancaui sp. nov. (Amphipoda, Niphargidae) – a new species thriving in sulfidic groundwaters in southeastern Romania

Can Environment Predict Cryptic Diversity? The Case of Niphargus Inhabiting Western Carpathian Groundwater

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