Morphological and microsatellite marker-based characterization and diversity analysis of novel vegetable soybean [Glycine max (L.) Merrill]

Pardeshi, Priya; Jadhav, P. V.; Sakhare, S. B.; Zunjare, Rajkumar U.; Rathod, Darasing R.; Sonkamble, Priti; Saroj, Ranjit; Varghese, Philips

doi:10.1007/s11033-023-08328-1

Cited by 4 publications

(4 citation statements)

References 31 publications

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“…Consequently, there is a pressing need to significantly improve the number of markers and standardize DNA fingerprints. SSR markers, which are abundant and randomly distributed in the genome, have demonstrated their effectiveness in identifying various crop varieties such as soybean [30], melon [39], apple [40], and grape [41]. However, with the growing number of cultivars requiring testing, more markers and higher detection throughput are necessary, along with the implementation of integration and sharing of fingerprint data.…”

Section: Discussionmentioning

confidence: 99%

“…These markers have played a crucial role in evaluating and characterizing the diversity of sesame germplasm from different origins and wild species [22][23][24][25][26], owing to the availability of the sesame whole genome sequence [27]. Additionally, SSR markers have also been applied to other vegetable crops such as pepper [28], tomato [29], and vegetable soybean [30]. Despite the widespread use of SSR markers in sesame research, no specific efforts have been made to evaluate the genetic diversity of Chinese modern sesame cultivars.…”

Section: Introductionmentioning

confidence: 99%

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Morphology and SSR Markers-Based Genetic Diversity Analysis of Sesame (Sesamum indicum L.) Cultivars Released in China

Wang,

Zhou,

Tang

et al. 2023

Agriculture

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Sesame is a highly valuable crop with significant global importance due to its nutritional and economic value. To better understand the genetic diversity of sesame genotypes at both molecular and morphological levels, a comprehensive study was conducted using 25 pairs of simple sequence repeat (SSR) primers and 56 biological traits in a collection of 183 sesame accessions, which comprised 101 Chinese cultivars, 62 landraces, and 20 exotic accessions. The analysis revealed a total of 166 SSR polymorphic bands, with an average of 6.64 bands per marker. The values of Shannon’s information index ranged from 0.2732 to 0.6497, indicating a moderate level of genetic diversity. The polymorphic information index ranged from 0.0859 to 0.6357, further supporting the presence of genetic variation. The average frequency of heterozygous genotypes was calculated as 0.34, suggesting a relatively narrow genetic diversity. The application of the unweighted pair group method with arithmetic averaging (UPGMA) clustering and principal component analysis allowed for the categorization of the 183 sesame accessions into three distinct groups. Furthermore, the genetic diversity coefficient of sesame germplasm is generally constrained, with no significant difference observed between the genetic diversity coefficient of Chinese cultivars and that of foreign resources. The results provide valuable data for various applications, including the breeding and promotion of new sesame cultivars in China, the protection of new variety rights, the inquiry and identification of DNA genetic information of cultivars, as well as the development and utilization of sesame germplasm resources both domestically and internationally.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Morphology and SSR Markers-Based Genetic Diversity Analysis of Sesame (Sesamum indicum L.) Cultivars Released in China

Wang,

Zhou,

Tang

et al. 2023

Agriculture

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“…The primary bene t of using microsatellites as genetic markers is their Mendelian inheritance as codominant markers, allowing for the detection of both homozygous and heterozygous dominance in plant breeding programs. Microsatellites are widely used genetic markers in breeding programs due to their high rates of polymorphism, abundance, and widespread distribution in the genome (Pardeshi et al, 2023). Despite being expensive to generate, they are still favored because they are e cient and cost-effective (Mabhaudhi and Modi, 2013).…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Taro (Colocasia Esculenta (L.) Schott) Using Simple Sequence Repeat (SSR) markers

Amoako,

Koomson,

Ashiagbor

et al. 2024

Preprint

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Taro, Colocasia esculenta (L.) Schott is one of the most valued crops for economic, medicinal, and nutritional roots and tubers globally. However, the crop has not received pronounced research attention to enhance its production and utilization potential, which has resulted in insufficient planting materials for utilization by breeders in Ghana. Genetic diversity characterization of taro at molecular level is important and crucial step for their utilization in breeding and conservation. Hence, this study was carried out to assess the genetic diversity and relationships of 26 accessions of Taro, C. esculenta (L.) Schott collected from Kwame Nkrumah University of Science and Technology, Ghana. A total of 38 putative alleles were amplified at 4 SSR loci with an average of 9.50 alleles per locus. PIC value ranged from 0.44 with primer XUQTEM 97 to 0.89 with primer XUQTEM 73 with an average value of 0.73. Expected Heterozygosity (He) ranged from 0.59 to 0.92. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) clustering using Jaccard's similarity coefficient grouped 26 accessions into eight clusters. The information generated from this investigation will be useful for researchers and breeders for their conservation, Molecular Breeding-based Research & Development and their sustainable future utilization.

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“…The PIs obtained from other countries were intensively tested for their phenotypic performances in the environmental conditions where the soybean variety would be developed. Therefore, for breeding purposes, it is highly necessary to characterize the introduced soybean genotypes to determine their properties both at morphological and molecular levels [26].…”

Section: Introductionmentioning

confidence: 99%

Diversity Analysis of 53 Soybean Accessions Introduced from China Based on Morphological Characteristics and SSR Markers

Dyah,

Wibisono,

Terryana

et al. 2024

Curr. Appl. Sci. Technol.

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Indonesia still faces challenges in meeting its national soybean demand. Genetic diversity can provide new resources to improve soybean production and quality. Genetic diversity of 53 soybean accessions introduced from China, based on morphological characteristics and 17 SSR markers, was analyzed in this study. Principal component analysis (PCA) conducted on morphological characters produced a total diversity value of 64.67% and identified four main components. Based on phylogenetic analysis and principal coordinate analysis (PCoA) two accessions showed low genetic similarity of 78% (China cult-55 and Mi yang niu mao huang), which indicated that they could be selected as parents for plant breeding programs. In addition, 772 SSR alleles at an average of 45 alleles per locus were detected. The average heterozygosity was 0.83, and the average polymorphic information content (PIC) value was 0.96. All SSR markers showed a PIC value > 0.8, indicating their informativeness in analyzing genetic diversity of soybean. The phylogenetic analysis indicated a genetic similarity of 82% and the accessions were grouped into two main clusters. The phylogenetic analysis depicted that several accessions could be grouped based on the growth type and origin. The results of morphological characterization and molecular markers in the analysis of genetic diversity are beneficial for selecting parental crosses when developing new varieties.

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Morphological and microsatellite marker-based characterization and diversity analysis of novel vegetable soybean [Glycine max (L.) Merrill]

Cited by 4 publications

References 31 publications

Morphology and SSR Markers-Based Genetic Diversity Analysis of Sesame (Sesamum indicum L.) Cultivars Released in China

Morphology and SSR Markers-Based Genetic Diversity Analysis of Sesame (Sesamum indicum L.) Cultivars Released in China

Molecular Characterization of Taro (Colocasia Esculenta (L.) Schott) Using Simple Sequence Repeat (SSR) markers

Diversity Analysis of 53 Soybean Accessions Introduced from China Based on Morphological Characteristics and SSR Markers

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