Morphological and mechanical characteristics of the reconstructed rat abdominal wall following use of a wet electrospun biodegradable polyurethane elastomer scaffold

Hashizume, Ryotaro; Fujimoto, Kazuro; Hong, Yi; Amoroso, Nicholas J.; Tobita, Kimimasa; Miki, Toshio; Keller, Bradley B.; Sacks, Michael S.; Wagner, William R.

doi:10.1016/j.biomaterials.2010.01.051

Cited by 76 publications

(92 citation statements)

References 43 publications

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“…8,24,25 Examination of the regenerating mucosa in the damaged middle-ear demonstrated increased synthesis of collagen III around the migrated donor cells at day 7 after transplantation, indicating active tissue remodeling. [46][47][48] Collagen type IVpositive regions were also detected under the epithelial cells during the same period, indicating the formation of the basement membrane. 46,49 Moreover, the migrated epithelial cells were PAS-positive, indicating that the cells produced mucus, which is a functional characteristic of middle-ear epithelial cells.…”

Section: Discussionmentioning

confidence: 87%

“…Collagen III-positive regions were detected mainly in the subepithelium after transplantation ( Figure 5E), indicating active remodeling of the middle-ear after mucosal tissue elimination. [46][47][48] Collagen IV-positive regions were detected under the donor cells ( Figure 5F), indicating attachment of donor cells to the ECM and basement membrane conformation. 46,49 Moreover, some of the cells found in the subepithelial layer were positive for PAS staining, indicating they were functional, producing some mucus protein ( Figure 5G).…”

Section: Analysis Of Mucosal Regeneration After Transplantationmentioning

confidence: 98%

“…41,44,45 A rabbit polyclonal antibody against collagen type III (dilution 1:400) (Abcam, Cambridge, UK) was used as a marker of elaboration of ECM component, 46,47 and a rabbit polyclonal antibody against collagen type IV (dilution, 1:50) (LSL, Tokyo, Japan) was used as a marker of basement membrane. 46,48 A monoclonal antibody against E-cadherin (dilution 1:1000) (BD Biosciences) was used as a marker of the adhesive core of adherens junctions.…”

Section: Antibodiesmentioning

confidence: 99%

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In situ tissue engineering with synthetic self-assembling peptide nanofiber scaffolds, PuraMatrix, for mucosal regeneration in the rat middle-ear

Akiyama¹,

Yamamoto‐Fukuda²,

Takahashi³

et al. 2013

IJN

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Middle-ear mucosa maintains middle-ear pressure. However, the majority of surgical cases exhibit inadequate middle-ear mucosal regeneration, and mucosal transplantation is necessary in such cases. The aim of the present study was to assess the feasibility of transplantation of isolated mucosal cells encapsulated within synthetic self-assembling peptide nanofiber scaffolds using PuraMatrix, which has been successfully used as scaffolding in tissue engineering, for the repair of damaged middle-ear. Middle-ear bullae with mucosa were removed from Sprague Dawley (SD) transgenic rats, transfected with enhanced green fluorescent protein (EGFP) transgene and excised into small pieces, then cultured up to the third passage. After surgical elimination of middle-ear mucosa in SD recipient rats, donor cells were encapsulated within PuraMatrix and transplanted into these immunosuppressed rats. Primary cultured cells were positive for pancytokeratin but not for vimentin, and retained the character of middle-ear epithelial cells. A high proportion of EGFP-expressing cells were found in the recipient middle-ear after transplantation with PuraMatrix, but not without PuraMatrix. These cells retained normal morphology and function, as confirmed by histological examination, immunohistochemistry, and electron microscopy, and multiplied to form new epithelial and subepithelial layers together with basement membrane. The present study demonstrated the feasibility of transplantation of cultured middle-ear mucosal epithelial cells encapsulated within PuraMatrix for regeneration of surgically eliminated mucosa of the middle-ear in SD rats.

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Section: Discussionmentioning

confidence: 87%

Section: Analysis Of Mucosal Regeneration After Transplantationmentioning

confidence: 98%

Section: Antibodiesmentioning

confidence: 99%

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In situ tissue engineering with synthetic self-assembling peptide nanofiber scaffolds, PuraMatrix, for mucosal regeneration in the rat middle-ear

Akiyama¹,

Yamamoto‐Fukuda²,

Takahashi³

et al. 2013

IJN

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“…Biaxial mechanical testing of intact skeletal muscle was performed as previously described (Hashizume et al ., 2010). Briefly, gastrocnemius muscles were harvested from young ( n = 7) and old ( n = 7) animals and placed in chilled ringers solution for 1 h prior to testing.…”

Section: Methodsmentioning

confidence: 99%

Aging of the skeletal muscle extracellular matrix drives a stem cell fibrogenic conversion

et al. 2017

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SummaryAge‐related declines in skeletal muscle regeneration have been attributed to muscle stem cell (MuSC) dysfunction. Aged MuSCs display a fibrogenic conversion, leading to fibrosis and impaired recovery after injury. Although studies have demonstrated the influence of in vitro substrate characteristics on stem cell fate, whether and how aging of the extracellular matrix (ECM) affects stem cell behavior has not been investigated. Here, we investigated the direct effect of the aged muscle ECM on MuSC lineage specification. Quantification of ECM topology and muscle mechanical properties reveals decreased collagen tortuosity and muscle stiffening with increasing age. Age‐related ECM alterations directly disrupt MuSC responses, and MuSCs seeded ex vivo onto decellularized ECM constructs derived from aged muscle display increased expression of fibrogenic markers and decreased myogenicity, compared to MuSCs seeded onto young ECM. This fibrogenic conversion is recapitulated in vitro when MuSCs are seeded directly onto matrices elaborated by aged fibroblasts. When compared to young fibroblasts, fibroblasts isolated from aged muscle display increased nuclear levels of the mechanosensors, Yes‐associated protein (YAP)/transcriptional coactivator with PDZ‐binding motif (TAZ), consistent with exposure to a stiff microenvironment in vivo. Accordingly, preconditioning of young fibroblasts by seeding them onto a substrate engineered to mimic the stiffness of aged muscle increases YAP/TAZ nuclear translocation and promotes secretion of a matrix that favors MuSC fibrogenesis. The findings here suggest that an age‐related increase in muscle stiffness drives YAP/TAZ‐mediated pathogenic expression of matricellular proteins by fibroblasts, ultimately disrupting MuSC fate.

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“…Historical data from our group were used to provide mechanical properties for the native rat abdominal wall. 25 …”

Section: Biaxial Mechanical Testingmentioning

confidence: 99%

An Elastomeric Patch Electrospun from a Blended Solution of Dermal Extracellular Matrix and Biodegradable Polyurethane for Rat Abdominal Wall Repair

Hong

Takanari

Amoroso

et al. 2012

Tissue Engineering Part C: Methods

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A biodegradable elastomeric scaffold was created by electrospinning a mixed solution of poly(ester urethane)urea (PEUU) and porcine dermal extracellular matrix (dECM) digest, with PEUU included to provide elasticity, flexibility, and mechanical support and dECM used to enhance bioactivity and biocompatibility. Micrographs and differential scanning calorimetry demonstrated partial miscibility between PEUU and dECM. With greater dECM content, scaffolds were found to possess lower breaking strains and suture retention strength, although initial modulus was greater with higher dECM concentrations. The hybrid scaffolds containing 0% to 50% dECM had tensile strengths of 5 to 7 MPa, breaking strains of 138% to 611%, initial moduli of 3 to 11 Mpa, and suture retention strengths of 35 to 59 MPa. When hydrated, scaffolds were found to contract markedly with 50% dECM content. When used in a rat full-thickness abdominal wall replacement model, no herniation, infection, or tissue adhesion was observed after 4 and 8 weeks with a scaffold containing 25% dECM or a control 100% PEUU scaffold. Scaffolds incorporating dECM were significantly thicker at the time of explant, with greater numbers of associated smooth muscle actin-positive staining cells than in the control, but minimal cellular infiltration and remodeling of the scaffold were detected regardless of dECM addition. The processing of dECM and PEUU from a mixed solution thus provided a scaffold with evidence of better bioactivity and with mechanical properties not achievable with digested dECM alone.

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Morphological and mechanical characteristics of the reconstructed rat abdominal wall following use of a wet electrospun biodegradable polyurethane elastomer scaffold

Cited by 76 publications

References 43 publications

In situ tissue engineering with synthetic self-assembling peptide nanofiber scaffolds, PuraMatrix, for mucosal regeneration in the rat middle-ear

In situ tissue engineering with synthetic self-assembling peptide nanofiber scaffolds, PuraMatrix, for mucosal regeneration in the rat middle-ear

Aging of the skeletal muscle extracellular matrix drives a stem cell fibrogenic conversion

An Elastomeric Patch Electrospun from a Blended Solution of Dermal Extracellular Matrix and Biodegradable Polyurethane for Rat Abdominal Wall Repair

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