Morphological and isoenzymatic differentiation of B-chronic lymphocytic leukaemia cells induced by phorbolester

Drexler, Hans G.; Klein, Mary Beth; Bhoopalam, Nirmala; Gaedicke, Gerhard; Minowada, Jun

doi:10.1038/bjc.1986.33

Cited by 36 publications

(4 citation statements)

References 16 publications

(15 reference statements)

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“…This isoenzyme could be induced by the phorbolester 12-0-tetradecanoylphorbol 13-acetate (TPA) and by a T-cell-conditioned medium in the myelomonocytic leukaemia cell lines ML-3, THP-1, U-937 and HL-60 undergoing differentiation to monocyte-/macrophage-like cells (24). All cases of B-CLL and CML which were treated with TPA and differentiated along the B-cell axis and to monocyte-macrophages, respectively, displayed the tartrate-resistant band after several days of exposure to the inducing agent (22,23). In the present study, only the monocytic variants FAB M4 and FAB M5 showed the tartrate-resistant isoenzyme, whereas the 'granulocytic' subtypes FAB Ml-M3 and the erythroleukaemia cases (FAB M6) did not.…”

Section: Discussioncontrasting

confidence: 47%

“…Cytochemical studies demonstrated tartrate-resistant acP activity in activated macrophages (12,19), in stimulated peripheral blood monocytes (20), in alveolar macrophages (20) and in the peripheral blood monocytes from patients with Gaucher disease (21), but not in unstimulated normal peripheral blood monocytes (20). Pre-viously, we showed that isoelectric focusing studies are more sensitive than cytochemical techniques in detecting tartrate-resistant acP (22,23). Future studies will determine whether there exist different (cell type-specific?)…”

Section: Discussionmentioning

confidence: 91%

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Monocyte‐associated acid phosphatase isoenzyme profiles as determined in acute myeloid leukaemia cells

Drexler

Gaedicke

Klein

et al. 1986

Scandinavian Journal of Haematology

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The acid phosphatase (acP) isoenzymes from the blast cells of 102 cases of acute myeloid leukaemia were separated by isoelectric focusing on horizontal polyacrylamide gels. The cases were classified on the basis of the FAB cooperative group criteria. Several single bands were combined into groups (I‐IV). An increase in the number of acP isoenzymes was noted which paralleled the assumed maturation along the granulocytic cell lineage from FAB M1 to FAB M3 and along the monocytic cell lineage from FAB M4 to FAB M5. One isoenzyme which was resistant to tartrate inhibition was found in 40% of the monocytic variants FAB M4 and M5, but not in the nonmonocytic cases FAB M1‐M3 and M6. This particular isoenzyme, which has been described as being characteristic for hairy cell leukaemia, also appears to be a marker of the monocyte/macrophage system and the respective neoplastic counterparts. The FAB M4 and M5 patients expressed a characteristic profile of group I isoenzymes which allows for the discrimination between monocytic and nonmonocytic cells.

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Section: Discussioncontrasting

confidence: 47%

Section: Discussionmentioning

confidence: 91%

Monocyte‐associated acid phosphatase isoenzyme profiles as determined in acute myeloid leukaemia cells

Drexler

Gaedicke

Klein

et al. 1986

Scandinavian Journal of Haematology

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“…Recently, late cardiovascular events have also been described in long-term survivors of testicular cancer treated with platinum-based chemotherapy [ 9 , 10 ]. In addition, vascular damage is known to be induced by therapeutic irradiation [ 11 , 12 ]. It has been found that CCS who received irradiation of the brain and/or neck are at increased risk for stroke [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Body mass index and annual increase of body mass index in long-term childhood cancer survivors; relationship to treatment

Brouwer

Gietema

Vonk

et al. 2011

Support Care Cancer

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PurposeEvaluation of body mass index (BMI) at final height (FH) and annual BMI increase in adult childhood cancer survivors (CCS) after treatment with anthracyclines, platinum, and/or radiotherapy.MethodsBMI (weight/height²) was calculated retrospectively from diagnosis until FH. The prevalence of underweight (BMI < 18.5 kg/m2) and overweight (BMI ≥ 25 kg/m2)/obesity (BMI ≥ 30 kg/m2) at FH was compared with age-matched controls. The association between underweight/overweight at FH and treatment was assessed by multivariate logistic regression. Annual BMI increase after treatment was assessed by multilevel analysis. Analyses were adjusted for age and underweight/overweight at diagnosis, and age at FH.ResultsAt FH the prevalence of overweight had not increased, while CCS experienced more underweight as compared to controls (14% vs. 4%, P < 0.001). Overweight at FH was associated with cranial/craniospinal radiotherapy (CRT; OR, 2.23; 95% CI, 1.17–4.26) and underweight at FH with anthracyclines > 300 mg/m2 (OR, 2.84; 95% CI, 1.33–6.06). Annual BMI increase was +0.47 (0.34–0.60) kg/m2/year. In CCS, the annual BMI increase was greater in those with CRT ≥ 30 Gy as compared with those with less or no CRT (+0.15 kg/m2/year [0.04–0.25 kg/m2/year], P = 0.008) and smaller in those with a higher cumulative anthracycline dose (−0.03 kg/m2/year [−0.05 to −0.0005 kg/m2/year] per 100 mg/m2, P = 0.046).ConclusionsAfter treatment with anthracyclines, platinum, and/or radiotherapy, CRT-treated survivors have more overweight at FH, and a greater annual BMI increase, while anthracycline-treated survivors have more underweight at FH and a lower annual BMI increase.

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“…In the first of these Ellis et al (1978) described the increased abundance of the I2 form in lymphocytes from patients with common acute lymphoblastic leukaemia (c-ALL), a characteristic that has been used as a marker in distinguishing common acute lymphoblastic leukaemia from other types ofleukaemia (Greaves, 1981). /8-N-Acetylhexosaminidase 12 was initially demonstrated in leukaemic cells by DEAE-cellulose chromatography, but it has also been detected in pre B-ALL, AUL, Null-ALL and AML as well as in c-ALL cells by isoelectric focusing (Drexler et al, 1984).…”

Section: Introductionmentioning

confidence: 99%

An enzyme with properties similar to those of β-N-acetylhexosaminidase S is expressed in the promyelocytic cell line HL-60

Emiliani

Beccari

Tabilio

et al. 1990

Biochemical Journal

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Extracts of the human promyelocytic cell line HL-60 contain a form of beta-N-acetylhexosaminidase that is not retained on columns of benzeneboronate-agarose ('phenylboronate-agarose') and has a pI value lower than that of beta-N-acetylhexosaminidase A. It is clearly distinct from beta-N-acetylhexosaminidase A in its behaviour on DEAE-cellulose columns, and it requires a higher concentration of salt for its elution. This 'extra' form has a higher ratio of activity towards 4-methylumbelliferyl beta-N-acetylglucosaminide 6-sulphate and 4-methylumbelliferyl beta-N-acetylglucosaminide than has beta-N-acetylhexosaminidase A and is less stable when heated at 50 degrees C. It has a pH optimum of 4.5 and is therefore not beta-N-acetylglucosaminidase C. Anti-(human beta-N-acetylhexosaminidase alpha-subunit) serum precipitated both beta-N-acetylhexosaminidase A and the 'extra' form, whereas an anti-(beta-subunit) serum precipitated beta-N-acetylhexosaminidase A but not the 'extra' form. Western blotting and immunodetection of polypeptides derived from the 'extra' form revealed a band corresponding in size to mature alpha-subunits. On the basis of this and of its behaviour on isoelectric focusing, chromatofocusing and its kinetic properties, we conclude that the 'extra' form is composed of alpha-subunits and resembles beta-N-acetylhexosaminidase S, the residual form in Sandhoff's disease.

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Morphological and isoenzymatic differentiation of B-chronic lymphocytic leukaemia cells induced by phorbolester

Cited by 36 publications

References 16 publications

Monocyte‐associated acid phosphatase isoenzyme profiles as determined in acute myeloid leukaemia cells

Monocyte‐associated acid phosphatase isoenzyme profiles as determined in acute myeloid leukaemia cells

Body mass index and annual increase of body mass index in long-term childhood cancer survivors; relationship to treatment

An enzyme with properties similar to those of β-N-acetylhexosaminidase S is expressed in the promyelocytic cell line HL-60

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