Morphological and immunohistochemical comparison of three rat prostate lobes (lateral, dorsal and ventral) in experimental hyperprolactinemia.

Słuczanowska-Głąbowska, Sylwia; Wylot, Marcin; Głąbowski, Wojciech; Piasecka, Małgorzata; Gącarzewicz, Dariusz

doi:10.2478/v10042-010-0074-3

Cited by 15 publications

(19 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These features tend to suggest a robust protein synthesis with the packaged secretory products released through the merocrine mechanism [3,33]. Merocrine secretion is established when the secretory granules are seen bulging or fusing to the apical cell membrane or are present in the luminal content [6,31]. From this study however, no structural evidence of merocrine secretion was seen in the coagulating gland.…”

Section: Discussionmentioning

confidence: 76%

The coagulating gland in the male greater cane rat (Thryonomys swinderianus): morphological and immunohistochemical features

et al. 2015

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 76%

The coagulating gland in the male greater cane rat (Thryonomys swinderianus): morphological and immunohistochemical features

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Finally, dark brown cells were considered to be positive under a light microscope and were counted throughout the microscopically magnified fields (× 400) of each articular cartilage section. According to previous studies [11][12][13], the average optical density (OD) of cells is the symbol of protein expression, so it was detected by ImagePro Plus6.0 system in each cartilage zone, followed by statistical analysis. Laser-scanning confocal microscopy.…”

Section: Histomorphologymentioning

confidence: 99%

Increased apoptosis in human knee osteoarthritis cartilage related to the expression of protein kinase B and protein kinase Cα in chondrocytes

Chen

Zhang

et al. 2012

Folia Histochem Cytobiol.

View full text Add to dashboard Cite

Protein kinase B (Akt) and protein kinase Ca (PKCa) play important roles in the regulation of cell apoptosis. The aim of this study was to investigate the expression of Akt and PKCa in chondrocytes of human knee osteoarthritic (OA) cartilage, further evaluating their role in chondrocyte apoptosis during OA progression. Human knee OA cartilages were obtained from 38 patients undergoing knee arthroplasty, which is the medium-late stage of OA. Healthy knee cartilages were obtained from 11 amputees. The samples taken from the condyle of femur were collected routinely for morphological, immunohistochemical and Western blot detection, respectively. Light microscopy and laser-scanning confocal microscopy were used for morphological observation. The optical density with computer image analysis evaluated the intensity of immunohistochemical reaction of Akt and PKCa in OA cartilage. Western blot detected the protein expression levels. The results indicated that Akt and PKCa were involved in OA progression, along with the increase of cell apoptosis. In OA cartilage, Akt decreased (p < 0.05) and PKCa increased (p < 0.05). There was a negative correlation and interaction between Akt and PKCa (r = -0.8). These results demonstrated that both Akt and PKCa are related to increased chondrocyte apoptosis in human OA cartilage. The correlation between human OA progression, the role of Akt and PKCa, and chondrocyte apoptosis allows for new therapeutic strategies to be considered.

show abstract

“…The growth and function of the prostate gland are stimulated by dihydrotestosterone (DHT) [1], which acts through androgen receptors to induce the expression of the genes associated with the synthesis of growth factors [2,3].…”

Section: Introductionmentioning

confidence: 99%

Hormone concentration, metabolic disorders and immunoexpression of androgen and estrogen-alpha receptors in men with benign prostatic hyperplasia and testosterone deficiency syndrome

Rył

Rotter

Słojewski

et al. 2015

Folia Histochem Cytobiol.

View full text Add to dashboard Cite

Introduction. A slight decrease in blood testosterone level in men is a physiological state associated with the aging. The aim of our study was to evaluate the occurrence of hormone and metabolic disorders, as well as the immunolocalization and immunoexpression of androgen receptors (AR) and estrogen-alpha receptors (ERa) in the prostates of men with benign prostatic hyperplasia (BPH) and coexisting testosterone deficiency syndrome (TDS). Material and methods. The study involved 150 men, diagnosed with and receiving pharmacological treatment for BPH. Concentrations of glucose, total cholesterol (TCh), high-density lipoproteins (HDL), low-density lipoproteins (LDL), and triglycerides (TG) were determined in blood serum. Serum concentrations of total testosterone (TT), free testosterone (FT), estradiol (E2), luteinizing hormone (LH), insulin (I), sex hormone-binding globulin (SHBG), dehydroepiandrosterone sulfate (DHEAS), and insulin-like growth factor 1 (IGF-1) were measured by ELISA. The number of AR-positive cells and ERa-positive cells were measured in prostate sections of men with BPH. Results. Patients eligible for transurethral resection of the prostate and TDS were significantly more likely to have higher abdominal circumference and higher serum levels of insulin and IGF-1 as well as lower levels of FT and SHBG than control subjects with BPH and no TDS. Quantitative analysis revealed 35.8% AR-positive columnar epithelial cells and 24.3% AR-positive stromal cells in prostates of BPH patients with TDS and 30.5% and 23.0%, respectively, in BPH patients without TDS. However, the differences between the study and the control groups were statistically not significant. In prostates of BPH patients with TDS the immunoexpression of ERa was observed in 2.88% of the columnar epithelial cells and 0.39% of stromal cells. In BPH patients without TDS ERa-positive cells were only found in 0.04% of columnar epithelial cells and 0.62% of prostatic stromal cells. Conclusions. Considering the statistically significantly higher levels of I and IGF-1 and larger abdominal circumference of men with BPH and TT deficiency, it can be supposed that visceral obesity and carbohydrate disorders may contribute to the reduction of testosterone concentration. The results of our study indicate a relationship between TT concentration in the plasma of patients with BPH and the percentage of AR-positive cells in the prostate.

show abstract

Morphological and immunohistochemical comparison of three rat prostate lobes (lateral, dorsal and ventral) in experimental hyperprolactinemia.

Cited by 15 publications

References 51 publications

The coagulating gland in the male greater cane rat (Thryonomys swinderianus): morphological and immunohistochemical features

The coagulating gland in the male greater cane rat (Thryonomys swinderianus): morphological and immunohistochemical features

Increased apoptosis in human knee osteoarthritis cartilage related to the expression of protein kinase B and protein kinase Cα in chondrocytes

Hormone concentration, metabolic disorders and immunoexpression of androgen and estrogen-alpha receptors in men with benign prostatic hyperplasia and testosterone deficiency syndrome

Contact Info

Product

Resources

About