Morphological and Histological Changes of Rabbit Vocal Fold after Steroid Injection

Jin, Hee-Jin; Lee, Sanghyuk; Seon-Uk, Lee，; Lee, Hyun-Sub; Jin, Sung-Min; Kim, Dong-Hun; Chae, Seung-Wan; Sohn, Jin-Hee

doi:10.1177/0194599813489657

Cited by 18 publications

(18 citation statements)

References 17 publications

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“…Steroids are very powerful antiinflammatory drugs; PSI can maintain a high concentration at Reinke's space, and reduce the inflammatory reaction of vocal fold lesions while preventing the potential systemic adverse effects of the steroid …”

Section: Discussionmentioning

confidence: 99%

Long-term efficacy of percutaneous steroid injection for treating benign vocal fold lesions: A prospective study

Lee

Park

2016

The Laryngoscope

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Section: Discussionmentioning

confidence: 99%

Long-term efficacy of percutaneous steroid injection for treating benign vocal fold lesions: A prospective study

Lee

Park

2016

The Laryngoscope

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“…Its size has been approximated to 8.6 × 5.5 mm at the level of the arytenoids (45,46), consistent with the dimensions of the human newborn larynx (47). Additionally, the literature demonstrates that rabbit larynges exhibit su cient biological similarity to humans and have been used in molecular and histological studies of the vocal folds (48)(49)(50)(51)(52). The rabbit larynx has also been used to characterize the physiological response to injury secondary to phonation (51,52) or laryngeal and vocal fold surgery (53)(54)(55).…”

Section: Introductionmentioning

confidence: 77%

RNA sequencing identifies transcriptional changes in the rabbit larynx in response to low humidity challenge

Bailey

Santos

Nascimento

et al. 2020

Preprint

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Background Voice disorders are a worldwide problem impacting human health, particularly for occupational voice users. Avoidance of surface dehydration is commonly prescribed as a protective factor against the development of dysphonia. The available literature inconclusively supports this practice and a biological mechanism for how surface dehydration of the laryngeal tissue affects voice has not been described. In this study, we used an in vivo male New Zealand white rabbit model to elucidate biological changes based on gene expression within the vocal folds from surface dehydration. Surface dehydration was induced by exposure to low humidity air (18.6% ± 4.3%) for 8 hours. Exposure to moderate humidity (43.0% ± 4.3%) served as the control condition. Ilumina-based RNA sequencing was performed and used for transcriptome analysis with validation by RT-qPCR. Results There were 103 genes identified through Cuffdiff with 64 genes meeting significance by both false discovery rate and fold change. Functional annotation enrichment and predicted protein interaction mapping showed enrichment of various loci, including cellular stress and inflammatory response, ciliary function, and keratinocyte development. Eight genes were selected for RT-qPCR validation. Matrix metalloproteinase 12 (MMP12) and macrophage cationic peptide 1 (MCP1) were significantly upregulated and an epithelial chloride channel protein (ECCP) was significantly downregulated after surface dehydration by RNA-Seq and RT-qPCR. Suprabasin (SPBN) and zinc activated cationic channel (ZACN) were marginally, but non-significantly down- and upregulated by RT-qPCR, respectively. Conclusions The data together support the notion that surface dehydration induces physiological changes in the vocal folds and justifies targeted analysis to further explore the underlying biology of compensatory fluid/ion flux and inflammatory mediators in response to airway surface dehydration.

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“…Its size has been approximated to 8.6×5.5mm at the level of the arytenoids (45,46), consistent with the dimensions of the human newborn larynx (47). Additionally, the literature demonstrates that rabbit larynges exhibit su cient biological similarity to humans and have been used in molecular and histological studies of the vocal folds (48)(49)(50)(51)(52). The rabbit larynx has also been used to characterize the physiological response to injury secondary to phonation (51,52) or laryngeal and vocal fold surgery (53)(54)(55).…”

Section: Introductionmentioning

confidence: 78%

RNA sequencing identifies transcriptional changes in the rabbit larynx in response to low humidity challenge

Bailey

Santos

Nascimento

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Voice disorders are a worldwide problem impacting human health, particularly for occupational voice users. Avoidance of surface dehydration is commonly prescribed as a protective factor against the development of dysphonia. The available literature inconclusively supports this practice and a biological mechanism for how surface dehydration of the laryngeal tissue affects voice has not been described. In this study, we used an in vivo male New Zealand white rabbit model to elucidate biological changes based on gene expression within the vocal folds from surface dehydration. Surface dehydration was induced by exposure to low humidity air (18.6% + 4.3%) for 8 hours. Exposure to moderate humidity (43.0% + 4.3%) served as the control condition. Ilumina-based RNA sequencing was performed and used for transcriptome analysis with validation by RT-qPCR. Results: There were 103 statistically significant differentially expressed genes identified through Cuffdiff with 61 genes meeting significance by both false discovery rate and fold change. Functional annotation enrichment and predicted protein interaction mapping showed enrichment of various loci, including cellular stress and inflammatory response, ciliary function, and keratinocyte development. Eight genes were selected for RT-qPCR validation. Matrix metalloproteinase 12 (MMP12) and macrophage cationic peptide 1 (MCP1) were significantly upregulated and an epithelial chloride channel protein (ECCP) was significantly downregulated after surface dehydration by RNA-Seq and RT-qPCR. Suprabasin (SPBN) and zinc activated cationic channel (ZACN) were marginally, but non-significantly down- and upregulated as evidenced by RT-qPCR, respectively. Conclusions: The data together support the notion that surface dehydration induces physiological changes in the vocal folds and justifies targeted analysis to further explore the underlying biology of compensatory fluid/ion flux and inflammatory mediators in response to airway surface dehydration.

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Morphological and Histological Changes of Rabbit Vocal Fold after Steroid Injection

Abstract: We demonstrated that the VF steroid injection resulted in no significant changes in morphology and histology of rabbit VF. However, steroid injection may induce several VF histological changes and these results are needed to be considered when treating humans.

Cited by 18 publications

References 17 publications

Long-term efficacy of percutaneous steroid injection for treating benign vocal fold lesions: A prospective study

Long-term efficacy of percutaneous steroid injection for treating benign vocal fold lesions: A prospective study

RNA sequencing identifies transcriptional changes in the rabbit larynx in response to low humidity challenge

RNA sequencing identifies transcriptional changes in the rabbit larynx in response to low humidity challenge

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