Morphological and growth altering effects of Cisplatin in C. albicans using fluorescence microscopy

Kesavan, Chandrasekhar; Malathi, R.; Ganesan, Natarajan

doi:10.1186/1476-0711-4-7

Cited by 13 publications

(6 citation statements)

References 11 publications

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“…C. albicans SC5314 was cultured in RPMI 1640 medium containing 11g (0, 0.5, and 1 × 10 –3 mg/mL) with an inoculums size of 10 6 cells/mL. Cells were cultured at 30°C with constant shaking and optical density at 600 nm were measured after incubating for 0, 1, 2, 4, 8, 12, 16, 24, and 32 h ( Kesavan et al, 2005 ). Three independent experiments were performed.…”

Section: Methodsmentioning

confidence: 99%

11g, a Potent Antifungal Candidate, Enhances Candida albicans Immunogenicity by Unmasking β-Glucan in Fungal Cell Wall

Huang

Liu

et al. 2020

Front. Microbiol.

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In the course of optimizing GPI biosynthesis inhibitors, we designed and synthetized a 2-aminonicotinamide derivative named 11g. After evaluating the antifungal activity of compound 11g in vitro, we investigated the influences of 11g on fungi immunogenicity. In addition, we also took advantage of murine systemic candidiasis model to investigate the protective effects of 11g in vivo. Results show that 11g exhibited potent antifungal activity both in vitro and in vivo. Further study shows that 11g caused the unmasking of fungi β-glucan layer, leading to stronger immune responses in macrophages through Dectin-1. These results suggest that 11g is a very promising antifungal candidate, which assists in eliciting stronger immune responses to help host immune system disposing pathogens. The discovery of 11g might expand the toolbox of fungal infection treatment.

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Section: Methodsmentioning

confidence: 99%

11g, a Potent Antifungal Candidate, Enhances Candida albicans Immunogenicity by Unmasking β-Glucan in Fungal Cell Wall

Huang

Liu

et al. 2020

Front. Microbiol.

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“…Identifying the mid-log phase is important to identify as it is the time point to compare a kānuka treatment condition to an untreated condition. Figure 3.10 shows the normal growth of P. acnes wherein exponential growth occurs for 60 hours with mid-log at 32 hours, which is consistent with other reputable studies (Kesavan et al, 2005;Lopez-Abarrategui et al, 2016).…”

Section: Normal Growth Of P Acnessupporting

confidence: 85%

The chemical biology of antimicrobial bioactivity derived from kānuka (Kunzea robusta)

Huia Haira

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Natural products are sourced from the environment where immense potential for bioactive compounds exist. Traditional medicine has explored these compounds, with many discoveries based on medicinal plants still in use today. The native flora in Aotearoa (New Zealand) contains many endemic species that are yet to be extensively investigated, for example Kunzea robusta or kānuka. Kānuka is considered a taonga species to Māori, the indigenous people of Aotearoa, due it to extensive use in Māori traditional medicine. Unlike its close relative mānuka, the bioactive potential of kānuka has yet to be extensively explored. Therefore, the aims of this thesis were to determine its medicinal potential by investigating the antifungal, antibacterial, and anti-P. acnes bioactivities of kānuka oil. Chemical profiles were also investigated, and statistical analyses correlated the chemical composition of kānuka oil extracts to the aforementioned bioactivities, thus identifying potential lead drug compounds.Methods: Using 99 samples of kānuka oil extracted from leaves collected from various locations in Te Tairawhiti via steam distillation, chemical profiles were determined using gas chromatography mass spectrometry (GC-MS) analysis. The growth of Candida albicans, methicillin-resistant Staphylococcus aureus (MRSA) and Propionibacterium acnes was evaluated in the presence of all kānuka extracts to represent each bioactivity. Multivariate statistical analysis was then used to identify the compounds within the kānuka extracts that positively correlate with the three aforementioned bioactivities.Results: Bioassays were conducted using minimum inhibitory concentration (MIC) of 5 x 10-5 % (v/v) for antifungal and anti-MRSA bioactivity and a MIC of 500 x 10 -5 % (v/v) for anti- P. acnes bioactivity. Seasonal and geographical variation for all three bioactivities was detected. Antifungal bioactivity assays revealed median percent growth of treated C. albicans cells to range between 38-54% with more variation in growth observed in season one (spring). The most potent extract in this experiment was seen in season one from land block H; however, in season two (autumn) land block H was the least potent (96%) and land block F was the most potent (32%). Antibacterial bioactivity assays revealed median percent growth of treated MRSA to range between 35-79% with variation consistent in both seasons. The most potent effects were seen in season one from land block H (28%) with the less effective coming from land block E where in some cases there was no effect on treated cell conditions. Finally, anti- P. acnes bioactivity assays revealed median percent growth of treated P. acnes cells to range between 62-73% with more variation observed in season two. The most potent effects were seen in season one from land block B (~43%) with the less effective coming from season two land block H (89%). GC-MS analysis distinguished α-pinene as the most abundant compound (~34%) and multivariate analysis identified lead compounds such as α-Murolene, isoamyl isovalerate for antifungal activity and compounds such as limonene and nerolidol for anti-MRSA and anti-P. acnes bioactivity. The unidentified compound, unknown was particularly interesting as it was the only compound to positively correlate to all three bioactivities.Conclusion: Kānuka oil extracted from Te Tairawhiti exhibits antifungal, anti-MRSA and anti-P. acnes properties that show seasonal and geographical variation. The results from this study provide scientific validation for the medicinal benefits of kānuka oil that can be further explored for pharmaceutical potential.

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“…Prior to the necessary treatments, all specimens were coded [ 42 ]. The operators put on protective eyewear before beginning the procedure [ 45 ].…”

Section: Methodsmentioning

confidence: 99%

“…The turbidimetric growth rates of both the control and study groups were calculated [ 45 – 48 ]. To make the inocula, a culture was diluted with 0.9% NaCl to 1.5x10 6 CFU/mL and left overnight.…”

Section: Methodsmentioning

confidence: 99%

Different wavelengths of laser: are they significant for treatment of denture stomatitis?: an in-vitro study

El-Din,

El-Sharkawy,

Abdelrahman

et al. 2024

BMC Oral Health

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Background Denture stomatitis (DS) is an inflammatory disorder that affects the mucosal surface underneath the dentures and frequently causes oral mucosal irritation, discomfort, and altered taste perception, which prevents people from consuming enough nutrients. One of the main causes of DS is an overgrowth of the fungus Candida albicans (C. albicans). A possible alternative treatment for Candida infections is thought to be laser therapy. The aim of this study was to evaluate how different wavelengths of laser would affect growth and pathogenic properties of Candida albicans. Methods A concentration of 106 viable cells/ml of Candida albicans were used in the preparation process. Four groups were created from the specimens. Culturing of the control group was completed with no intervention. The other 3 groups received laser radiation for 60 seconds at a power of 1W. The 2nd and 3rd groups were irradiated with diode laser at a wavelength of 940 nm and 980 nm respectively. The 4th group was irradiated with Nd-YAG laser at a wavelength of 1064 nm. Turbidimetric growth was defined as variations in the optical density of fungal growth. These measures were made at three different times: baseline, 48 hours, and 72 hours. Results In both groups of diode laser, the growth of Candida albicans showed no remarkable differences at baseline, after 48 and 72 hours using a power of 1 W and duration of 60 seconds. The Nd-YAG group showed significant increase in optical density after 48 hrs then significant decrease after 72 hrs. The optical density values in the control group showed no notable difference between the control and diode study groups at different time periods. However, the Nd:YAG group showed a statistically significant difference compared to the control and the 2 diode laser groups. Conclusions Different laser parameters have a different effect on growth and pathogenic properties of Candida albicans. Diode laser therapy with wavelengths 940 and 980 nm used in continuous mode with power of 1 W for duration of 60 seconds can result in proliferation of Candida albicans instead of destroying them. Nd:YAG laser, used in pulsed mode, with power of 1 W for a duration of 60 seconds can be used to destroy Candida albicans and therefore, can be used as an effective treatment for denture stomatitis.

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Morphological and growth altering effects of Cisplatin in C. albicans using fluorescence microscopy

Cited by 13 publications

References 11 publications

11g, a Potent Antifungal Candidate, Enhances Candida albicans Immunogenicity by Unmasking β-Glucan in Fungal Cell Wall

11g, a Potent Antifungal Candidate, Enhances Candida albicans Immunogenicity by Unmasking β-Glucan in Fungal Cell Wall

The chemical biology of antimicrobial bioactivity derived from kānuka (Kunzea robusta)

Different wavelengths of laser: are they significant for treatment of denture stomatitis?: an in-vitro study

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