Morphological and growth alterations in Vero cells transformed by cisplatin

Abstract

Cisplatin is an antineoplastic agent used to treat solid tumours, such as ovarian, testicular and bladder tumours. However, studies in vitro and in vivo have shown that cisplatin is mutagenic, genotoxic and tumorigenic in other tissues and organs. In this work, we examined the effect of cisplatin on Vero cells, a fibroblast-like cell line. The morphological characteristics were investigated using phase contrast microscopy, scanning electron microscopy and the actin cytoskeleton was labelled with fluorescein isothiocyanate-phalloidin. Cell proliferation was assessed based on the growth curve. Cultured Vero cells treated with cisplatin showed behavioural and morphological alterations associated with cellular transformation. The transformed cells grew in multilayers and formed cellular aggregates. The proliferation and morphological characteristics of the transformed cells were very different from those of control ones. Since transformed Vero cells showed several characteristics related to neoplastic growth, these cells could be a useful model for studying tumour cells in vitro.

Gemcitabine/Cisplatin Treatment Induces Concomitant SERTAD1, CDKN2B and GADD45A Modulation and Cellular Changes in Bladder Cancer Cells Regardless of the Site of TP53 Mutation

“…Actin polymerization and remodeling play pivotal roles in regulating the morphology and phenotype of a malignant cell. Moreover, these actin filaments play important roles in mitosis and cell signaling [9]. Thus, alteration or decrease in cellular microfilaments could have compromised cellular viability.…”

“…In addition to gene expression profiling, morphological data are also important for establishing diagnoses and indicating prognoses while treating a patient for cancer. Changes in cell morphology and growth that were characteristic of neoplastic development were detected in Vero cells that were treated with cisplatin [9]. Moreover, morphological changes, including rounding and shrinkage in a glioma cell line (GBM-8401), were visualized after 24 and 48 h of incubation with AITC, suggesting that this agent is an effective treatment option [10].…”

Evaluation of the protective effect of aqueous extract of fenugreek against cisplatin-induced toxicity in Vero cells

“…However, after three successive subcultures, the treated Vero cells revealed morphological and structural alterations that ended in cell detachment and death [32]. The increase in the cytoplasmic area of cells of group II could be due to vacuolization of the cytoplasm, whereas that of the nuclear area could be due to the transient proliferative effect of cisplatin, as explained by other researchers.…”

“…In contrast, other investigators have suggested that these vesicles are due to the elimination exocytosis of secretory material to the extracellular environment [32]. It was reported that vacuolization in the cells is generally seen as an adaptive physiological response, presumably for 'damage limitation', but very little is known about the intracellular homeostatic mechanisms that operate to restore the status quo.…”

The Effect of Non Freeze-dried Hydrogel-CHA on Fibroblast Proliferation

“…Observation of the length of time for the application according to this research, it has conducted by previous studied. 16 They said that the fi broblasts did not change the morphological characteristics after incubation days 1, 2 and day 3. Another studied determined that during the process osteoblastic cells grown on the scaffold and suffered proliferation, differentiation, and promotes bone mineralization.…”