Morphological and genetic identification of formalin-fixed gobioid larvae and description of postflexion larvae of Paragunnellichthys sp. and Ctenogobiops feroculus

Hanahara, Nozomi; Miyamoto, Kazuaki; Oka, Shin-ichiro

doi:10.1007/s10228-020-00769-z

Cited by 3 publications

(2 citation statements)

References 24 publications

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“…Due to limited information, traditional morphological identification restricts not only species identification but also the understanding of larval and juvenile stages in many fish species. Molecular technology overcomes the bottleneck in larval and juvenile species identification [14][15][16][17][18], enabling a broader understanding of species diversity. This study combined DNA barcoding with dynamic research methods to identify species at various stages and sizes.…”

Section: Introductionmentioning

confidence: 99%

Morphological Development and DNA Barcoding Identification of Pholis fangi Larvae and Juveniles in the Yellow Sea

Liu,

Zhang,

et al. 2024

Fishes

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Pholis fangi is a small, bottom-dwelling fish species endemic to the Yellow Sea and Bohai Sea in China. While previous research has focused on its population biology and molecular structure, little is known about the early developmental stages of this species. In this study, larval and juvenile P. fangi specimens were collected from the Sheyang Sea Area, Jiangsu Province, in 2017. Morphological features were examined using microscopy, and DNA barcoding was conducted to confirm species identification. The research documented detailed changes in yolk sac, fin development, and melanophore distribution patterns across larval and juvenile stages of P. fangi. Comparative analysis with other Pholis species revealed that melanophore distribution is a key distinguishing characteristic, allowing effective differentiation between larval and juvenile stages, as well as between Pholis species. This study provides valuable insights into the early life history of P. fangi, contributing to a better understanding of the genus Pholis. The findings demonstrate the utility of combining traditional morphological observation and molecular techniques for accurate species identification, particularly during the critical larval and juvenile developmental phases.

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Section: Introductionmentioning

confidence: 99%

Morphological Development and DNA Barcoding Identification of Pholis fangi Larvae and Juveniles in the Yellow Sea

Liu,

Zhang,

et al. 2024

Fishes

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“…As eel goby eggs have yet to be reported in either natural or aquarium conditions, their spawning habit is unknown, although the induced spawning and embryonic development of Odontamblyopus lacepedii (Temminck and Schlegel 1845) have been described and it is suspected to lay eggs in the walls of burrows (Dotsu & Takita, 1967). Larvae have been collected by set nets, midwater trawling, small seine nets and light traps and are therefore predicted to swim freely in water columns (Dôtu, 1957, 1958; Hanahara et al ., 2021; Leis & Carson‐Ewart, 2000; Maeda & Tachihara, 2014). The larval morphologies, described in Dôtu (1957, 1958), Harada and Suharti (2000), Leis and Carson‐Ewart (2000), Leis and Trnski (1989), Okiyama (2014), and Ruple (1984), show moderate‐sized eyes at the mid‐lateral position of the head, like other gobies, which reduce in size after settlement.…”

Section: Introductionmentioning

confidence: 99%

Larval study revealed diversity and life‐history traits of crypto‐benthic eel gobies

Maeda

Hanahara

Uehara³

et al. 2022

Journal of Fish Biology

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Because adult and juvenile eel gobies usually hide within the burrows of muddy substrates, their diversity and life history have not yet been fully elucidated. We investigated larval specimens of the eel gobies collected on Okinawa Island in southern Japan. The genus Trypauchenopsis was previously thought to consist of only one species, but our larval collection identified two species, Trypauchenopsis limicola and Trypauchenopsis intermedia, distinguished by their species-specific melanophore arrangements and differences in their fin-ray counts. Taenioides kentalleni were previously known from only two specimens worldwide. A third specimen of this species has now been added from the larval collection. In addition to the three species above, Taenioides gracilis and Caragobius urolepis were identified and the larval morphologies of the five species were described for the first time. All the larvae collected in the present study were at late postflexion stage. T. limicola, T. intermedia and T. gracilis were presumably collected in the estuaries and beaches when approaching their adult habitats at the end of pelagic life. They were 8.5-10.3 mm in standard length, and otolith analysis suggests that their pelagic larval durations are a little longer than 1 month (average 34-37 days). The larval occurrence suggested that the spawning season of T. limicola is May-December, when the water temperature is warmer than approximately 20 C. Our work reveals that studying the larval stage can provide new information on the taxonomy and life history of the elusive cryptobenthic fish.

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Genetic and morphological identification of formalin fixed, preserved larval fishes; can we have the best of both worlds?

Appleyard

Maher

Miskiewicz

et al. 2022

Journal of Experimental Marine Biology and Ecology

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Morphological and genetic identification of formalin-fixed gobioid larvae and description of postflexion larvae of Paragunnellichthys sp. and Ctenogobiops feroculus

Cited by 3 publications

References 24 publications

Morphological Development and DNA Barcoding Identification of Pholis fangi Larvae and Juveniles in the Yellow Sea

Morphological Development and DNA Barcoding Identification of Pholis fangi Larvae and Juveniles in the Yellow Sea

Larval study revealed diversity and life‐history traits of crypto‐benthic eel gobies

Genetic and morphological identification of formalin fixed, preserved larval fishes; can we have the best of both worlds?

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