Morphological and Genetic Characterization of Micropropagated Field Grown Plants of Aloe vera L.

Das, Ashok Kumar; Haque, Sk Moquammel; Ghosh, Biswajit; Nandagopal, Krishnadas; Jha, Timir Baran

doi:10.3329/ptcb.v25i2.26257

Cited by 14 publications

(7 citation statements)

References 33 publications

(24 reference statements)

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“…However, the loss of essential oil towards maturity could be observed as a result of lysis and senescence of the oil glands instead of the metabolic regulation. The similar trend has been reported in Salvia sclarea …”

Section: Resultsmentioning

confidence: 99%

“…These genetic changes can involve movement of transposable elements and changes at the level of a single DNA nucleotide that might occur in a coding region. It has been reported earlier that RAPD analysis of Allium cepa showed a unique band in independent gametoclones which was proposed to have arisen due to a DNA sequence highly vulnerable to tissue‐culture‐induced mutations . Thus, molecular changes can reflect stable changes in the genome which can be used to breed for stable, desirable phenotypes.…”

Section: Resultsmentioning

confidence: 99%

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Production dynamics in relation to ontogenetic development and induction of genetic instability through in vitro approaches in Pelargonium graveolens: A potential essential oil crop of commercial significance

Pandith

Dhar

Wani

et al. 2017

Flavour & Fragrance J

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Rose‐scented geranium (Pelargonium graveolens L’ Hért.) is a fragrant and bushy essential oil bearing crop of significant industrial and commercial importance. In an attempt to overcome the constraints imposed by sexual sterility for genetic improvement and restricted environmental adaptability of Pelargonium, the present study was aimed at exploring new niches for its cultivation in northern most sub‐tropical part of India (Jammu). The flowering stage was found to be the optimum stage for harvesting in relation to essential oil quantity (0.16–0.18%, Fresh Weight Basis; FWB) and quality (geraniol 18% and citronellol 37%) during different phenophases of plant growth. Dynamics of essential oil turnover correlated well with the glandular trichome ontogeny using scanning electron microscopy (SEM). The present study also entailed induction of somaclonal variations via in vitro organogenesis. Organogenetic callus induction was achieved on B5 (Gamborg's) basal medium supplemented with kinetin/2,4‐dichlorophenoxyacetic acid (Kn/2,4‐D) and kinetin/indole‐3‐butyric acid (Kn/IBA). Frequency of callus induction ranged from 54–85%. Field established in vitro regenerated plants/somaclones displayed appreciable variability in fresh leaf biomass (365–2020 g, FWB), essential oil content (0.06%–0.26%) and quality (geraniol and citronellol as major constituents). To evaluate the genetic instability of calliclones, RAPD (Random Amplification of Polymorphic DNA) markers were used to assess the genetic basis of variability. Further, the identity of Pelargonium was confirmed by DNA bar‐code analyses using three plant based barcoding loci (ITS, rbcL and psbA/trnH). They may be used as a standard reference system for varietal identification and protection of Intellectual Property Rights (IPR). Overall, the present investigation demonstrates the feasibility of Pelargonium cultivation in sub‐tropical niches as a short duration winter crop, with desirable essential oil content and composition for commercial purposes. It also establishes a viable method for induction of variability for genetic amelioration of sexually sterile crop.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Production dynamics in relation to ontogenetic development and induction of genetic instability through in vitro approaches in Pelargonium graveolens: A potential essential oil crop of commercial significance

Pandith

Dhar

Wani

et al. 2017

Flavour & Fragrance J

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“…In this way, we can distinguish three large groups of Aloe: acauleas (without a trunk), subcauleas (visible trunk but with a reduced size), (Davis et al, 1994;Das et al, 2015;Sayed et al, 2016).…”

Section: Botanical Classificationmentioning

confidence: 99%

Recent update on the medicinal properties and use of Aloe vera in the treatment of various ailments

Mahor¹,

Ali²

2016

Biosci. Biotech. Res. Comm

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Aloe vera (Aloe barbadensis) an herb is widely used in Ayurvedic, Homoeopathic and Allopathic streams for its marvelous medicinal properties. This plant is one of the richest natural sources of health for mammals including human beings. The chemistry of the plant has revealed the presence of more than 200 different biologically active substances, which include antimicrobial, antibacterial, antifungal, antiviral, activities of the nonvolatile constituents of the leaf gel. Aloe species are widely distributed in the African and the eastern European continents, and are spread almost throughout the world. The genus Aloe has more than 400 species but few, such as A. vera, Aloe ferox, and Aloe arborescens, are globally used for trade. Many biological properties associated with Aloe species are contributed by inner gel of the leaves. anti diabetic, anti-infl ammatory, peptic ulcers, antitumor, anticancer Properties, activity effects on the Immune System, adverse reactions, Laxative effects, wound healing, antiseptic, vitamins, minerals, enzymes, amino acids, stress, sugars. It is known to help slow down the appearance of wrinkles and actively repair the damaged skin cells that cause the visible signs of aging. Aloe is a powerful detoxifi er, antiseptic and tonic for the nervous system. Aloe vera gel contains a large range of vitamins even vitamin B12, Vitamin A, contains B-Group vitamins, Vitamin C, Vitamin E and folic acid. Aloe vera gel contains important ingredients including 19 of the 20 amino acids needed by the human body and seven of the eight essential ones that just cannot be made. The plant leaves and inner gel contains numerous help it has potential to cure sunburns and minor cuts, and even skin cancer and acts as also acts as extremely powerful laxative. Various parts of the plant have different effects on the body. The present review is an attempt to highlight the proven research related botanical and pharmacological medicinal properties of Aloe vera.

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“…When germination is carried out in vitro , the seed germination percentage can reach 60–70% (Das et al, 2016). …”

Section: Propagation Techniquesmentioning

confidence: 99%

Propagation Techniques and Agronomic Requirements for the Cultivation of Barbados Aloe (Aloe vera (L.) Burm. F.)—A Review

2016

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Barbados aloe (Aloe vera (L.) Burm. F.) has traditionally been used for healing in natural medicine. However, aloe is now attracting great interest in the global market due to its bioactive chemicals which are extracted from the leaves and used in industrial preparations for pharmaceutical, cosmetic, and food products. Aloe originated from tropical and sub-tropical Africa, but it is also now cultivated in warm climatic areas of Asia, Europe, and America. In this review, the most important factors affecting aloe production are described. We focus on propagation techniques, sustainable agronomic practices and efficient post harvesting and processing systems.

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Morphological and Genetic Characterization of Micropropagated Field Grown Plants of Aloe vera L.

Cited by 14 publications

References 33 publications

Production dynamics in relation to ontogenetic development and induction of genetic instability through in vitro approaches in Pelargonium graveolens: A potential essential oil crop of commercial significance

Production dynamics in relation to ontogenetic development and induction of genetic instability through in vitro approaches in Pelargonium graveolens: A potential essential oil crop of commercial significance

Recent update on the medicinal properties and use of Aloe vera in the treatment of various ailments

Propagation Techniques and Agronomic Requirements for the Cultivation of Barbados Aloe (Aloe vera (L.) Burm. F.)—A Review

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