Morphological and genetic characterization ofPterygodermatites(Paucipectines)zygodontomis(Nematoda: Rictulariidae) fromNecromys lasiurus(Rodentia: Sigmodontinae) from Uberlândia, Brazil
Abstract:Pterygodermatites (Paucipectines) zygodontomis, a nematode parasite of the small intestine of the rodent Necromys lasiurus, from Uberlândia, Minas Gerais state, Brazil, was analysed by light and scanning electron microscopy. Additionally, phylogenies were inferred from the mitochondrially encoded cytochrome c oxidase I gene (MT-CO1). Details of the helminth surface, such as the oral aperture, cephalic papillae, papillae in the posterior region of the body and longitudinal cuticular elements represented by spin… Show more
“…Among the helminths described in N. lasiurus , nematodes are the most frequently reported (Vicente et al ., 1997; Anderson et al ., 2009). In general, studies of helminths from Brazilian rodents often reported nematodes (Vicente et al ., 1997; Anderson et al ., 2009; Simões et al ., 2010, 2011, 2012, 2017; Cardoso et al ., 2016; Tavares et al ., 2017; Costa et al ., 2018); however, most studies are case reports. Studies of acanthocephalans from rodents, including taxonomy, ecology, molecular biology and phylogeny, are still scarce.…”
Section: Introductionmentioning
confidence: 99%
“…During an investigation of hantavirus cases (Limongi et al , 2013), rodents were captured in the municipality of Uberlândia (18°55'07″S, 48°17'19″W), in the state of Minas Gerais, south-eastern Brazil, within the Cerrado biome. Helminths were recovered for an ecological study of the metacommunity structure of the helminth community of the rodent N. lasiurus (Costa et al ., 2018). From that study, we recovered specimens of the genus Moniliformis , which had never been previously reported for this host.…”
A new species of Moniliformis Travassos, 1915 (Acanthocephala: Moniliformidae) is described from the hairy-tailed bolo mouse, Necromys lasiurus Lund, 1840 (Cricetidae: Sigmondontinae), captured in the Brazilian Cerrado, in Uberlândia, state of Minas Gerais, Brazil. The specimens were studied by light and scanning electron microscopy. Molecular phylogenies were inferred from partial nuclear large subunit ribosomal RNA gene sequences and partial mitochondrial cytochrome c oxidase subunit I gene. The new species is distinguished from other moniliformid species by the number of rows and number of hooks per row, size of the proboscis, size of the eggs, host species and geographical distribution. Molecular phylogenies and genetic distances analyses demonstrated that Moniliformis necromysi sp. n. forms a well-supported monophyletic group with sequences of other species of Moniliformis and is distinguished from them, which agrees with the morphological characteristics, allocating the new species to this genus and to the family Moniliformidae Van Cleave, 1924. This is the first moniliformid acanthocephalan described from a wild rodent in Brazil.
“…Among the helminths described in N. lasiurus , nematodes are the most frequently reported (Vicente et al ., 1997; Anderson et al ., 2009). In general, studies of helminths from Brazilian rodents often reported nematodes (Vicente et al ., 1997; Anderson et al ., 2009; Simões et al ., 2010, 2011, 2012, 2017; Cardoso et al ., 2016; Tavares et al ., 2017; Costa et al ., 2018); however, most studies are case reports. Studies of acanthocephalans from rodents, including taxonomy, ecology, molecular biology and phylogeny, are still scarce.…”
Section: Introductionmentioning
confidence: 99%
“…During an investigation of hantavirus cases (Limongi et al , 2013), rodents were captured in the municipality of Uberlândia (18°55'07″S, 48°17'19″W), in the state of Minas Gerais, south-eastern Brazil, within the Cerrado biome. Helminths were recovered for an ecological study of the metacommunity structure of the helminth community of the rodent N. lasiurus (Costa et al ., 2018). From that study, we recovered specimens of the genus Moniliformis , which had never been previously reported for this host.…”
A new species of Moniliformis Travassos, 1915 (Acanthocephala: Moniliformidae) is described from the hairy-tailed bolo mouse, Necromys lasiurus Lund, 1840 (Cricetidae: Sigmondontinae), captured in the Brazilian Cerrado, in Uberlândia, state of Minas Gerais, Brazil. The specimens were studied by light and scanning electron microscopy. Molecular phylogenies were inferred from partial nuclear large subunit ribosomal RNA gene sequences and partial mitochondrial cytochrome c oxidase subunit I gene. The new species is distinguished from other moniliformid species by the number of rows and number of hooks per row, size of the proboscis, size of the eggs, host species and geographical distribution. Molecular phylogenies and genetic distances analyses demonstrated that Moniliformis necromysi sp. n. forms a well-supported monophyletic group with sequences of other species of Moniliformis and is distinguished from them, which agrees with the morphological characteristics, allocating the new species to this genus and to the family Moniliformidae Van Cleave, 1924. This is the first moniliformid acanthocephalan described from a wild rodent in Brazil.
“…zygodontomis, Grossmann (2015) reported higher prevalence, abundance, and intensity in N. lasiurus than in the present study. This species was reported in Rhipidomys mastacalis, also in Uberlˆandia (Cardoso, 2016;Costa et al, 2017). Quentin (1967) reported this species in N. lasiurus and in Ca.…”
Section: Helminths Species and New Recordsmentioning
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“…to GenBank (accession number: KY462830.1). The latter sequence obtained from a specimen isolated from Mastomys coucha in South Africa is nearly identical (99%) to a partial (631 bp) 18S sequence of P. muricola (KP760162) from a gorilla of Central African Republic, but has a sequence identity of only 95% to P. numidica (KT894812, KT894811) not included for our analysis due to its controversial morphological assignment (Costa et al 2018 ). Fig.…”
Section: Resultsmentioning
confidence: 97%
“…The sequences from P. muricola built a separate group with CO1 sequence identities of 84.99–85.62% (472 bp) to Mastophorus from Mus . Misidentifications, erroneously assigned sequences like those from P. numidica sequences obtained from specimens collected from Oxymycterus in Brazil (de Barros 2015 ) assigned to the genus Prostospirura without describing details or illustrating their specimens, while belonging to the genus Mastophorus (Costa et al 2018 , 2022 ), are challenging to conclude and might promote confusion in the classification. While further studies should focus on data generation, validation (by associated taxonomic annotation) and phylogenetic analysis of reference sequences to clarify the confusion of Mastophorus with Protospirura , our work provides a deeper insight into the morphological and phylogenetic diversity of the genus Mastophorus.…”
Mastophorus muris (Gmelin, 1790) is a globally distributed parasitic nematode of broad range mammals. The taxonomy within the genus Mastophorus and the cryptic diversity among the genus are controversial among taxonomists. This study provides a detailed morphological description of M. muris from Mus musculus combined with a molecular phylogenetic approach. Moreover, descriptions and molecular data of M. muris from non-Mus rodents and wildcats complement our findings and together provide new insights into their taxonomy. The analysis of M. muris was based on light microscopy and scanning electron microscopy. The morphological description focused on the dentition pattern of the two trilobed pseudolabia. Additionally, we described the position of the vulva, arrangement of caudal pairs of papillae, spicules and measured specimens from both sexes and the eggs. For the molecular phylogenetic approach, we amplified the small subunit ribosomal RNA gene and the internal transcribed spacer, and the cytochrome c oxidase subunit 1. Mastophorus morphotypes based on dentition patterns and phylogenetic clustering indicate a subdivision of the genus in agreement with their host. We recognize two groups without a change to formal taxonomy: One group including those specimens infecting Mus musculus, and the second group including organisms infecting non-Mus rodents. Our genetic and morphological data shed light into the cryptic diversity within the genus Mastopohorus. We identified two host-associated groups of M. muris. The described morphotypes and genotypes of M. muris allow a consistent distinction between host-associated parasites.
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