Morphological and genetic characterization of Porrocaecum angusticolle (Molin, 1860) (Nematoda: Ascaridomorpha) from the common buzzard Buteo buteo (Linnaeus) (Accipitriformes: Accipitridae) in Czech Republic

Guo, Ning; Sitko, Jiljí; Chen, Hui-Xia; Li, Liang

doi:10.1016/j.parint.2021.102365

Cited by 5 publications

(6 citation statements)

References 20 publications

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“…Therefore, molecular techniques, such as DNA sequencing, are the most reliable methods to identify anisakid larvae (Lin et al 2007 ; Tunya et al 2020 ). Several studies have demonstrated the utility of nuclear and mitochondrial DNA markers such as large ribosomal DNA (28S), internal transcribed spacer (ITS), and cytochrome c oxidase subunit 1 (cox1) and subunit 2 (cox2) for the identification of ascaridoid worms (Li et al 2017 ; Zhang et al 2018 ; Guo et al 2021 ). In Egypt, some previous studies recorded Anisakis spp.…”

Section: Introductionmentioning

confidence: 99%

Morphological and molecular identification of third-stage larvae of Anisakis typica (Nematoda: Anisakidae) from Red Sea coral trout, Plectropomus areolatus

et al. 2023

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Anisakidosis is a foodborne zoonotic infection induced by members of the family Anisakidae via the consumption of raw or undercooked fish such as sushi and sashimi. Identifying anisakid larval species is critical for the epidemiology and diagnosis of diseases caused by them. This study aimed at identifying Anisakis larvae collected from marine fish in Egyptian waters based on morphological characteristics and molecular analysis. Thirty marine fish bar-cheeked trout, Plectropomus maculatus, were collected from Hurghada, Red Sea, Egypt, to investigate larval nematodes of the genus Anisakis. The larvae were detected encapsulated in the peritoneal cavity and muscle of the fish host. This examination revealed that anisakid larvae naturally infected 19 fish specimens with a prevalence of 63.33% and a mean intensity of 4.1 ± 0.40. Most of them (68 larvae: 71.57%) were found in the musculature. Morphological and morphometric analyses using light and scanning electron microscopy revealed a head region with a prominent boring tooth, inconspicuous lips, and a characteristic protruded cylindrical mucron. All larvae in this study possessed the same morphology as Anisakis Larval type I. Molecular analysis based on ITS region using maximum likelihood and Bayesian phylogenetic methods confirmed them as Anisakis typica. This is the first study to identify A. typica larvae from the commercial fish bar-cheeked trout P. maculatus in Egyptian waters using morphological and molecular methods.

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Section: Introductionmentioning

confidence: 99%

Morphological and molecular identification of third-stage larvae of Anisakis typica (Nematoda: Anisakidae) from Red Sea coral trout, Plectropomus areolatus

et al. 2023

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“…Previous studies have mainly focused on the morphological and genetic characterization of cases of P. angusticolle infections in B. buteo , Strigiformes , A. clanga , A. chrysaetos , and other birds ( Kijewska et al., 2002 ; Santoro et al., 2010 ; Santoro et al., 2012 ; Tomás et al., 2017 ; Guo et al., 2021 ). Therefore, information on the lifecycle of P. angusticolle is still lacking.…”

Section: Resultsmentioning

confidence: 99%

“…Briefly, P. angusticolle is classified into Eukaryota, Metazoa, Nematoda, Choromadorea, Rhabditida, Ascarididae, and Porrocaecum. Previous studies have reported P. angusticolle infections in Europe, mainly in Italy ( Santoro et al., 2010 ), Portugal ( Tomás et al., 2017 ), the Czech Republic ( Kijewska et al., 2002 ; Guo et al., 2021 ), Germany ( Honisch and Krone, 2008 ), and Spain ( Sanmartín et al., 2004 ; Santoro et al., 2012 ). Several birds species have been reported to be infected by P. angusticolle , including Buteo buteo ( Kijewska et al., 2002 ; Santoro et al., 2010 ; Guo et al., 2021 ), Strigiformes , Aquila clanga , Aquila chrysaetos , Accipiter gentilis , Accipiter nisus , Aquila pomarine , Aquila rapax , Accipiter striatus , Buteo jamaicensis , Buteo lagopus , Buteo platypterus , Circus aeruginosus , Circus cyaneus , Circaetus gallicus , Elanius caeruleus , Haliaeetus albicilla , Haliastur indus , Milvus milvus , Milvus migrans , Pernis apivorus , Pandion haliaetus ( Santoro et al., 2012 ), Sparrowhawk ( Min et al., 2021 ), Circus aeruginosus ( Kijewska et al., 2002 ), Accipiter gentilis , Accipiter nisus , Buteo lagopus , Falco subbuteo , Milvus migrans , Pandion haliaetus ( Honisch and Krone, 2008 ), Tyto alba , and Strix aluco ( Sanmartín et al., 2004 ).…”

Section: Introductionmentioning

confidence: 99%

First reported Porrocaecum angusticolle infection in Griffon vulture (Gyps fulvus) in China

Liu

Zhang

2023

Front. Cell. Infect. Microbiol.

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This present study is the first case of a Porrocaecum angusticolle (P. angusticolle) infection reported in Griffon vulture (Gyps fulvus) in China. This study aimed to identify the nematode species and explore the genetic evolution of worms infecting Gyps fulvus (G.fulvus). Clinical examination revealed several milky white parasites in the stomach and intestinal tract. Polymerase chain reaction and partial 18S gene sequencing analyses identified these worms to be P. angusticolle (SD isolates). Further phylogenetic analyses revealed that they shared the highest genetic identity (99.9%) with a P. angusticolle isolate (EU004820.1) from Germany. Our study is the first report on the identification and characterization of P. angusticolle infecting G.fulvus in China, based on clinical findings and molecular diagnosis. Therefore, our study provides novel insights for the diagnosis of P. angusticolle infections and the prevention of nematode transmission in wild and domestic animals.

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“…Most of the pathogens identified in this study are commonly found in European diurnal and nocturnal raptors [8,23,24,30,35,[37][38][39][40][41][42][43][44][45][46].…”

Section: Resultsmentioning

confidence: 99%

“…The identification of parasite genus/species was performed based on keys or descriptions given in previous studies [40,41,48,59,[73][74][75][76][77][78][79][80][81][82][83][84].…”

Section: Parasitological Analysismentioning

confidence: 99%

Parasites, Bacteria, and Associated Pathological Changes in the Digestive System of Diurnal and Nocturnal Raptors in Central Italy

et al. 2021

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The knowledge of raptor pathogens and associated lesions may be extremely important to enhancing raptor conservation efforts and reducing pathogen spillover to humans and domesticated animals and vice versa. Parasite infections of the digestive system and associated bacteria and pathological changes were evaluated in deceased diurnal and nocturnal raptors in central Italy. Overall, the prevalence of parasites (nematodes, cestodes, trematodes, acanthocephalans, and protozoa) identified in the examined birds was 72.41%, and most of the positive raptors (71.42%) showed multiple parasite infections. Among bacteria, Salmonella typhimurium, Salmonella enterica subspecies diarizonae, Escherichia coli, Clostridium perfringens, Yersinia enterocolitica, and Pasteurella multocida were identified. The results obtained showed that both parasites and bacteria may cause severe lesions in the digestive system of diurnal and nocturnal raptors; parasites and bacteria may concur in causing these lesions; most severe lesions are caused by the interaction of multiple pathogens, both parasites and bacteria; and the same pathogen taxa are frequently associated with the same pathological changes. This study is the first report of S. typhimurium and S. enterica subspecies diarizonae in Buteo buteo, while Andracantha mergi, Spirocerca spp., Sarcocystis dispersa, Sarcocystis columbae, and Eumonospora spp. were recorded for the first time in Italy.

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Morphological and genetic characterization of Porrocaecum angusticolle (Molin, 1860) (Nematoda: Ascaridomorpha) from the common buzzard Buteo buteo (Linnaeus) (Accipitriformes: Accipitridae) in Czech Republic

Cited by 5 publications

References 20 publications

Morphological and molecular identification of third-stage larvae of Anisakis typica (Nematoda: Anisakidae) from Red Sea coral trout, Plectropomus areolatus

Morphological and molecular identification of third-stage larvae of Anisakis typica (Nematoda: Anisakidae) from Red Sea coral trout, Plectropomus areolatus

First reported Porrocaecum angusticolle infection in Griffon vulture (Gyps fulvus) in China

Parasites, Bacteria, and Associated Pathological Changes in the Digestive System of Diurnal and Nocturnal Raptors in Central Italy

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