Morphological and functional characteristics of cryopreserved multipotent mesenchymal stromal cells from bone marrow, adipose tissue and tendons

Волкова, Н. А.; Yukhta, Mariia; Goltsev, Anatoliy N.

doi:10.22494/cot.v4i2.64

Cited by 11 publications

(7 citation statements)

References 20 publications

(26 reference statements)

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“…Primary suspension of cells from biopsies of adipose and cartilage tissues of rats (n = 5) was obtained by enzymatic digestion (Volkovа et al, 2016). To do this, tissue samples were washed with Hanks' solution (PAA, Austria) with gentamicin (150 μg/mL) (Farmak, Ukraine) and incubated in a type II collagenase solution (1.5 mg/mL) (PanEco, Russia) at 4 °C for 18 hours.…”

Section: Methodsmentioning

confidence: 99%

“…Cell cultures derived from all studied sources have phenotype of precursor cells of mesenchymal origin. The MMSCs of cartilage tissue are characterized by a greater capacity for colony formation and proliferation, and lower capacity for directed differentiation than MMSCs from bone marrow and adipose tissue (Volkovа et al, 2016). In another previous study (Volkovа et al, 2019) we investigated the effect of growth and differentiation factors on CrMMSCs from bone marrow, adipose and cartilage tissues.…”

Section: Introductionmentioning

confidence: 99%

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Course correction of adjuvant arthritis with cryopreserved multipotent mesenchymal stromal cells

Vvedenskyi

Волкова

Yukhta

et al. 2021

Regul. Mech. Biosyst.

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Rheumatoid arthritis is an inflammatory autoimmune disease that occurs as a result of impaired immune tolerance, leading to an aberrant immune response to autologous antigens. Multipotent mesenchymal stromal cells (MMSCs) and the biologically active substances they produce can promote the activation of regenerative processes in the organism not only by direct cell differentiation, but also due to their inherent trophic and immunosuppressive potentials. The aim of the study was to experimentally evaluate changes in the course of the acute phase of adjuvant arthritis upon local and generalized administration of cryopreserved MMSCs from adipose and cartilage tissues. The results of histological, imunohistochemical and biochemical studies showed that the animals of the control group throughout the observation period developed an inflammatory process, which manifested in joint swelling (increased arthritis index), leukocytosis, spread of chondrocyte-free zones, weakening of staining, loss of clarity of cartilage tissue contours, increased content of cyclooxygenase-2, reduced glycosaminoglycan content and total antioxidant defense system activity. At the same time, the local administration of cryopreserved MMSCs from adipose and cartilage tissues contributed to the normalization of the structural and functional organization, content of glycosaminoglycans and cyclooxygenase-2 with complete recovery of blood parameters. Less pronounced regeneration processes in articular cartilage occurred under generalized administration of cryopreserved MMSCs from adipose and cartilage tissues in comparison with the local method. However, the difference between the control and experimental groups indicates the ability of cryopreserved MMSCs to influence the intensity of regenerative processes in damaged cartilage tissue with both methods of administration. Comparative evaluation of the use of cryopreserved MMSCs from adipose and cartilage tissues showed the absence of significant changes in the studied indicators. These data can be used to substantiate and develop methods of arthritis treatment in clinical practice.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Course correction of adjuvant arthritis with cryopreserved multipotent mesenchymal stromal cells

Vvedenskyi

Волкова

Yukhta

et al. 2021

Regul. Mech. Biosyst.

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“…Раніше нами було показано, що клітинні культури отримані з кісткового мозку та альтернативних джерел, -сухожильної та жирової тканин, -мають імунофенотип клітин попередників мезенхімального походження [14], зберігають здатність до проліферації, міграції та синтезу колагену І типу [15].…”

Section: вступunclassified

Influence of Growth Factors on Cryopreserved Mesenchymal Stromal Cells

Волкова¹,

Yukhta²,

Goltsev³

2019

Fiziol Zh

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У роботі було досліджено вплив факторів росту та диференціювання на кріоконсервовані мезенхімальні стромальні клітини, отримані з кісткового мозку, жирової та хрящової тканин. Додавання до культурального середовища фактора росту фібробластів (20 нг/мл) призводило до підвищення у 2,6 та 2,1 раза проліферативного потенціалу кріоконсервованих мезенхімальних стромальних клітин (КрМСК) з кісткового мозку та жирової тканини. Застосування трансформуючого ростового фактора β (10 нг/мл) збільшувало синтез колагену ІІ типу у 7,2 та 6,1 раза і глікозоаміногліканів у 5,1 та 2,6 рази відповідно для КрМСК з кісткового мозку та жирової тканини. Проліферативна активність, синтез колагену ІІ типу та глікозаміногліканів КрМСК з хрящової тканини не зазнавали змін під впливом досліджених факторів, проте характеризувалися порівняно більшою активністю синтетичних процесів відносно КрМСК з кісткового мозку та жирової тканини. Отримані результати можуть бути використані для обґрунтування та розробки методики застосування кріоконсервованих мезенхімальних стромальних клітин у клінічній практиці для лікування уражень тканин опорно-рухового апарату. Ключові слова: мезенхімальні стромальні клітини; фактори росту; проліферація; колаген; глікозаміноглікани.

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“…Therefore, the study of the characteristics of cryopreserved cultures is an important trend in modern biotechnology. In previous studies, we investigated the morphofunctional characteristics of cryopreserved MMSCs obtained from the bone marrow, adipose and tendon tissues, and found that adipose tissue-derived MMSCs (AT-MMSCs) had a greater ability to colony formation, proliferate, and have the same ability to direct differentiation compared with MMSCs from bone marrow [20].…”

mentioning

confidence: 99%

Potentiation of cryopreserved rat adipose-derived multipotent mesenchymal stromal cells by BMP-12 in vitro for the treatment of tendinopathy

Волкова¹,

Yukhta²,

Goltsev³

2018

JCOT

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Bone morphogenetic proteins (BMP), which are part of TGF-b superfamily, have a stimulating effect on bone formation, as well as the proliferation of tenocytes and fibroblasts, which form the tendon tissue.The purpose of the study was to determine the effect of BMP-12 on the morphofunctional characteristics of cryopreserved rat adipose-derived multipotent mesenchymal stromal (AT-MMSCs) and to investigate the effects of the use of AT-MMSCs pre-cultured with BMP-12 on the tendinopathy model.Materials and methods. MMSCs were obtained from the adipose tissue of rats. Cryopreservation was carried out under the protection of 10 % DMSO with the addition of 20 % fetal bovine serum at a cooling rate of 1 °C/min to -80 °C and subsequent transfer to liquid nitrogen. When culturing AT-MMSCs, the BMP-12 was added at the concentration of 50 ng/mL. The ability of cells to proliferation (by MTT-test) and the synthesis of collagen I and III types were evaluated. Rats with modeled degenerative-dystrophic injury to the Achilles tendons were locally administered with 0.25·106 AT-MMSCs or AT-MMSCs+BMP-12 in the defect zone. The animals injected with saline provided control group. On the 21st day after the treatment, a histological, immunofluorescence and biomechanical studies were performed.Results. The application of BMP-12 results in the decrease in proliferation of AT-MMSCs along with an increase in the relative number of cells that synthesize collagen I and III types relative to AT-MMSCs cultivated under standard conditions. The injection of AT-MMSCs promotes the activation of regenerative processes in damaged tendons. The use of AT-MMSCs+BMP-12 accelerates the histological structure, strength, and the content of collagen I and III types in the Achilles tendons of animals with degenerative-dystrophic damage compared to the cells without BMP-12.Conclusions BMP-12 can be used as a stimulating agent for the tenogenic differentiation of AT-MMSCs before transplantation into the damaged tendon tissue

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Morphological and functional characteristics of cryopreserved multipotent mesenchymal stromal cells from bone marrow, adipose tissue and tendons

Cited by 11 publications

References 20 publications

Course correction of adjuvant arthritis with cryopreserved multipotent mesenchymal stromal cells

Course correction of adjuvant arthritis with cryopreserved multipotent mesenchymal stromal cells

Influence of Growth Factors on Cryopreserved Mesenchymal Stromal Cells

Potentiation of cryopreserved rat adipose-derived multipotent mesenchymal stromal cells by BMP-12 in vitro for the treatment of tendinopathy

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