Morphological and cytogenetical characterization of ‘Dalle Khursani’: a polyploid cultivated Capsicum of India

Jha, Timir Baran; Saha, Priya; Nath, Sayantani; Das, Ashok Kumar; Jha, Sumita

doi:10.1016/j.scienta.2016.12.005

Cited by 12 publications

(9 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, knowledge of variation in the genome size, genetic plasticity, level of adulteration, fruit quality, pungency, size, and color is very important parameters for breeding advancement programs in chillies. For deciphering variation in Capsicum species, morphological indicators have played a big role, among which flower and fruit characteristics are most important [47][48][49][50], in which biochemical, physiological, and molecular aspects are also extensively investigated [3,[51][52][53][54]. Though morphological and biochemical characters are credible scores for evaluating variation in Capsicum species but are also subject to change under different environmental conditions [55,56], therefore accessing genetic diversity using molecular markers is more advantageous because molecular markers are phenotypically neutral and not regulated by environmental conditions.…”

Section: Discussionmentioning

confidence: 99%

Exploring the genetic makeup and population structure among Capsicum accessions for crop improvement and breeding curriculum insights

Haq

Dubey²,

Prerna

et al. 2022

Journal of Genetic Engineering and Biotechnology

View full text Add to dashboard Cite

Background Capsicum or chilli is an important crop in India which exhibits immense structural and genetic variations reflecting their intra- and inter-specific relationships. The aim of this study was to establish relationships amongst 54 Capsicum accessions through analysis of genetic and population structure using ISSR markers. Results Out of 19, successful DNA amplifications were shown by 7 ISSR primers and a total of 80 bands were identified ranging between 8 and 14 with an average of 11.43 bands/primer. A significant degree of polymorphic information content (PIC), discriminating power (DP), resolving power (RP), effective multiplex ratio (EMR), and marker index (MI) were identified as 0.39, 0.70, 6.40, 5.88, and 2.30, respectively, using ISSR markers in chillies. The cross-transferability ranged from 8.0 to 72.15% with an average of 52.63% among chillies. Amongst genetic information, grand mean values were 0.264, 0.180, 0.376, 0.296, and 0.180, which correspond to Shannon’s information index (I), expected heterozygosity (He), Nei’s gene diversity, total diversity among species (Ht), diversity within species (Hs), respectively. Further, the coefficients of gene differentiation (Gst) and gene flow (Nm) were 0.393 and 0.773, representing higher genetic variation among the population which was confirmed by analysis of molecular variance (AMOVA). Conclusion ISSR markers represented a potent system for the estimation of relationships or variation studies and generated information useful for planning crop management and improvement strategies in chilli breeding.

show abstract

Section: Discussionmentioning

confidence: 99%

Exploring the genetic makeup and population structure among Capsicum accessions for crop improvement and breeding curriculum insights

Haq

Dubey²,

Prerna

et al. 2022

Journal of Genetic Engineering and Biotechnology

View full text Add to dashboard Cite

show abstract

“…Pickersgill (1977) and Jha et al (2012) both described what they believed to be natural tetraploids. Molecular characterization of the cultivar Dalle Khursani, a perennial shrub from West Bengal, India, confirmed it as an allotetraploid rich in GC heterochromatin (Jha et al, 2017). This cultivar differed at the cytomolecular and morphological levels from the diploid species of the C. annuum complex (C. annuum, C. chinense, and C. frutescens), leading the authors to hypothesize that it is a natural interspecific hybrid.…”

Section: Capsicum Cytogeneticsmentioning

confidence: 95%

“…This cultivar differed at the cytomolecular and morphological levels from the diploid species of the C. annuum complex (C. annuum, C. chinense, and C. frutescens), leading the authors to hypothesize that it is a natural interspecific hybrid. Further analysis using fluorescent in situ hybridization (FISH) and genomic in situ hybridization is expected to facilitate the identification of the parental species (Jha et al, 2017).…”

Section: Capsicum Cytogeneticsmentioning

confidence: 99%

Capsicum—An Abbreviated Compendium

Jarret¹,

Barboza²,

Batista³

et al. 2019

J. Amer. Soc. Hort. Sci.

View full text Add to dashboard Cite

Pepper (Capsicum L.) is a major vegetable and spice crop worldwide. Global production of both fresh and dried fruit continues to increase steadily in terms of area harvested and yield. Various topics are addressed in this review, including recent additions to and clarification of Capsicum taxonomy, genetic resources of Capsicum, cytogenetic studies, the current status of our understanding of the mechanisms affecting the biosynthesis of capsaicinoids, the use of gene mutations to elucidate carotenoid biosynthetic pathways and their regulation, and recent advances in whole-genome sequencing and assembly.

show abstract

“…The genetic characterization of some Capsicum spp. has been previously carried out using agro-morphological and quality attributes ( Dutta et al., 2017 ; Vaishnavi et al., 2018 ), cytogenetics ( Jha et al., 2017 ), and different generations of markers such as sodium dodecyl-sulfate polyacrylamide gel electrophoresis ( Mena et al., 2019 ); randomly amplified polymorphic DNA (RAPD) markers ( Bosland and Baral, 2007 ), simple sequence repeats (SSR) markers ( Adluri et al., 2017 ; Colney et al., 2018 ; Baruah et al., 2019 ), and internal transcribed spacer sequence of nuclear ribosomal DNA ( Purkayastha et al., 2012 ); and sequencing of the ribosomal RNA (rRNA) gene-internal transcribed (ITS) region ( Kehie et al., 2016 ). Due to genome-wide coverage, robust and high reproducibility, co-dominant inheritance, high polymorphism with multiple alleles per locus, and transferability between species, SSR markers have been widely used in fingerprinting, analysis of genetic diversity and population structure, association mapping, and linkage mapping ( Lee et al., 2004 ; Minamiyama et al., 2006 ; Mimura et al., 2012 ; Yumnam et al., 2012 ; Sugita et al., 2013 ; Dhaliwal et al., 2014 ; Lee et al., 2016 ; Adluri et al., 2017 ; Colney et al., 2018 ; Baruah et al., 2019 ; Pereira-Dias et al., 2019 ; Solomon et al., 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Ecology, genetic diversity, and population structure among commercial varieties and local landraces of Capsicum spp. grown in northeastern states of India

Verma,

Pandey,

Thirugnanavel

et al. 2024

Front. Plant Sci.

View full text Add to dashboard Cite

Northeastern states of India are known for unique landraces of Capsicum spp. with geographical indications. However, little information is available about these valuable landraces of chillies. Surveys and collections were carried out in niche areas to find out their ecology and diversity through morphological traits and molecular analysis using microsatellite markers. Our result characterized the ecology of niche areas as cool (11.0°C–20.7°C) and humid (>60% relative humidity) climates for dalle-chilli (Capsicum annuum L.); mild-warm (12.2°C–28.6°C) and humid for king-chilli (C. chinense Jacq.); and cool to warm (11.3°C–33.1°C) and humid for bird’s eye chilli (C. frutescens L.) during the crop period. The canonical correspondence analysis has shown the significant impact of temperature on the agro-morphological traits and distribution of the landraces in their niche areas. A wide variability was observed for different quantitative traits and yield attributing characters (fruit length, diameter, weight, and yield), showing high heritability (97.0%–99.0%), and genetic advance as a percentage of the mean (119.8%–434.0%). A total of 47 SSR markers used for the molecular analysis generated 230 alleles, ranging from 2 (HPMSE-7) to 10 (HPMSE-5), with an average of 4.89 alleles per locus. The average polymorphism information content was also high (0.61) and ranged from 0.20 (HPMSE-7) to 0.85 (CAMS-91). The observed average heterozygosity was lower than the expected value. Analysis of molecular variance has shown significant variation within (69%) and between (31%) of the populations of Capsicum spp. Based on Nei’s genetic distance, bird’s eye chilli and king-chilli were found to be closer to each other, whereas dalle-chilli, a tretraploid species, was closer to hot pepper (C. annuum). However, the flower size of dalle-chilli was large and found closer to king-chilli in color and differs from C. chinense due to the presence of calyx teeth. For quality traits, landraces king-chilli, dalle-chilli, and bird’s eye chilli have shown 2.8, 2.0, and 1.4 times higher average capsaicin and 0.46, 0.25, and 0.22 times higher average oleoresin content over the hot pepper, respectively. The knowledge of ecology and diversity can be used in identifying new areas for production, selection of elite lines, conservation, and crop improvement.

show abstract

Morphological and cytogenetical characterization of ‘Dalle Khursani’: a polyploid cultivated Capsicum of India

Cited by 12 publications

References 23 publications

Exploring the genetic makeup and population structure among Capsicum accessions for crop improvement and breeding curriculum insights

Exploring the genetic makeup and population structure among Capsicum accessions for crop improvement and breeding curriculum insights

Capsicum—An Abbreviated Compendium

Ecology, genetic diversity, and population structure among commercial varieties and local landraces of Capsicum spp. grown in northeastern states of India

Contact Info

Product

Resources

About