Morphological and cytochemical determination of cell death by apoptosis

Taatjes, Douglas J.; Sobel, Burton E.; Budd, Ralph C.

doi:10.1007/s00418-007-0356-9

Cited by 184 publications

(147 citation statements)

References 63 publications

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“…This is not unusual as other results suggest that the ultrastructural features described as typical of apoptotic cells would be, in some cases, specific of the cell type (Taatjes et al, 2008).…”

Section: Discussionsupporting

confidence: 75%

Testicular histomorphometry and the proliferative and apoptotic activities of the seminiferous epithelium in Syrian hamster (Mesocricetus auratus) during regression owing to short photoperiod

et al. 2015

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SUMMARYDuring the non-breeding season some animals exhibit testicular atrophy, decreased testicular weight and reduced seminiferous tubule diameter accompanied by depletion of the seminiferous epithelium. Some cellular factors involved in this depletion are changes in germ cell proliferation and apoptosis. In the Syrian hamster this depletion has been studied histologically and in terms of the involvement of proliferation and apoptosis in the seminiferous epithelium of fully regressed testes. The objectives of this study included the histomorphometrical characterization of the testis and the determination of the proliferative and apoptotic activity of germ cells in the seminiferous epithelium during testicular regression owing to short photoperiod. The study was performed using conventional light microscopy (hematoxylin and eosin), proliferating cell nuclear antigen and terminal deoxynucleotidyl transferase (TdT)-mediated dUTP in situ nick end labelling staining, image analysis software, and transmission electron microscopy in three established regression groups: mild regression (MR), strong regression (SR), and total regression (TR). Morphometrically a gradual decrease in total tubular area and in the testicular, tubular, and epithelial volumes was observed during testicular regression. Interstitial and luminal volumes decreased from the MR group onwards. The tubular length decreased from MR to SR. As regards spermatogonial proliferation, only an initial decrease in proliferative activity was observed, whereas apoptotic germ cell activity increased throughout regression. The number of germ cells studied decreased throughout the process of testicular regression. In conclusion, testicular regression in Syrian hamster comprises two histomorphometrical phases, the first involving a decrease in seminiferous tubular diameter and volume and the second involving shortening of the seminiferous tubule and a decrease in interstitial volume. At the cellular level, there is an initial decrease in proliferation and increase in apoptosis involving all germ cells. At the end of regression, the proliferative and apoptotic activities of the spermatogonia recover the values observed prior to regression in preparation for recrudescence.

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Section: Discussionsupporting

confidence: 75%

Testicular histomorphometry and the proliferative and apoptotic activities of the seminiferous epithelium in Syrian hamster (Mesocricetus auratus) during regression owing to short photoperiod

et al. 2015

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“…This is consistent with the sequence of apoptosis, in which the activation of caspase-3 precedes DNA fragmentation in apoptotic cell death. 16,19 Although most cementocytes were double positive, there were a few cementocytes positive only for ssDNA. The following are two plausible explanations: (1) caspase-3-negative, ssDNA-positive cells might reach the final stage of apoptosis, and (2) they might undergo caspase-independent cell death because recent studies have indicated a cell death pathway without the involvement of caspases (i.e., the caspase-independent pathway).…”

Section: Discussionmentioning

confidence: 97%

“…Immunohistochemical staining was performed as described previously. 16 After deparaffinization, the sections were treated with proteinase K, rinsed with distilled water, and then incubated in 50% formamide diluted in distilled water at 568C for 20 minutes. The sections were rinsed with cold phosphate buffered saline (PBS) for 5 minutes and incubated with 3% Block Ace (Dainippon Sumitomo Pharma, Tokyo, Japan) at room temperature for 20 minutes.…”

Section: Methodsmentioning

confidence: 99%

Cementocyte cell death occurs in rat cellular cementum during orthodontic tooth movement

Matsuzawa

Toriya

Nakao

et al. 2016

The Angle Orthodontist

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Objective: To clarify the mechanism of root resorption during orthodontic treatment, we examined cementocyte cell death and root resorption in the cellular cementum on the pressure side during experimental tooth movement. Materials and Methods: Using 8-week-old male Wistar rats, the right first molar was pushed mesiobuccally with a force of 40 g by a Ni-Ti alloy wire while the contralateral first molar was used as a control. Localization and number of cleaved caspase-3-positive and single-stranded DNA (ssDNA) -positive cells were evaluated using dual-label immunohistochemistry with anticleaved caspase-3 and anti-ssDNA antibodies. In addition, tartrate-resistant acid phosphatase (TRAP)-positive cells in the cellular cementum were evaluated using TRAP histochemical staining. Results: Caspase-3-and ssDNA-positive cells appeared at 12 hours, but were restricted to the compressed periodontal ligament (PDL) and not the cellular cementum. Cleaved caspase-3-positive cementocytes were observed in the cellular cementum adjacent to the compressed PDL on day 1. From days 2 to 4, the number of caspase-3-and ssDNA-positive cementocytes increased. TRAP-positive cells appeared on the cellular cementum at the periphery of the hyalinized tissue on day 7, and resorption progressed into the broad surface of the cementum by day 14. Conclusion: Cementocytes adjacent to the hyalinized tissue underwent apoptotic cell death during orthodontic tooth movement, which might have been associated with subsequent root resorption. (Angle Orthod. 2017;87:416-422)

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“…In relation with apoptosis, the lectin histochemistry was proposal some years ago as a method for the histological identification of apoptotic cells [42]. This minireview presents evidence for this and also some of the results that we have obtained recently regarding the detection of apoptotic cells in the seminiferous epithelium.…”

Section: Apoptosis and Glycoconjugatesmentioning

confidence: 76%

Identification of Apoptotic Cells by Means of Lectin Histochemistry: State of the Art Review

Seco-Rovira

Beltrán-Frutos²,

Ferrer³

et al. 2015

J Cytol Histol

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Apoptosis is a cellular phenomenon of great importance in the cellular homeostasis of tissues and organs. For many years diverse techniques have been developed for the identification and differentiation of normal and apoptotic cells, but only in cells isolated in cultures as in histology sections. These techniques are based on the modifications that cells suffer during the process of apoptosis. One of these changes is linked with modifications of glycoconjugates in the glycocalix of cells, which allows the use of lectins for identification of these apoptotic cells. In this review, we first present the data obtained to date with this approach for the detection of apoptotic cells with lectins, both using flow cytometry and, especially, cytochemical methods. Secondly we comment on the results obtained in the detection of apoptotic cells with classical lectin histochemistry using histological sections of the seminiferous epithelium. These results open the possibility of using lectins in normal and pathological organs as a tool to identify "in situ" apoptotic cells both in the initial and late phases of apoptosis although further studies are needed in other organs to determine their usefulness and effectiveness over others identification techniques of "in situ" apoptosis.

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Morphological and cytochemical determination of cell death by apoptosis

Cited by 184 publications

References 63 publications

Testicular histomorphometry and the proliferative and apoptotic activities of the seminiferous epithelium in Syrian hamster (Mesocricetus auratus) during regression owing to short photoperiod

Testicular histomorphometry and the proliferative and apoptotic activities of the seminiferous epithelium in Syrian hamster (Mesocricetus auratus) during regression owing to short photoperiod

Cementocyte cell death occurs in rat cellular cementum during orthodontic tooth movement

Identification of Apoptotic Cells by Means of Lectin Histochemistry: State of the Art Review

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