Morphological and Chemical Changes of Deciduous Enamel Produced by Er:YAG Laser, Fluoride, and Combined Treatment

Zamudio-Ortega, C M; Contreras‐Bulnes, Rosalía; Vilchis, Rogelio José Scougall; Morales‐Luckie, Raúl A.; Olea-Mejía, Oscar; Rodríguez‐Vilchis, Laura Emma; García-Fábila, María Magdalena

doi:10.1089/pho.2013.3622

Cited by 21 publications

(51 citation statements)

References 29 publications

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“…This could have occurred at the expense of increases in C and F at%. These results are comparable with those reported by ZamudioOrtega et al [25], who applied acidulated phosphate fluoride (APF), although their study was performed on the smooth surfaces of temporary teeth.…”

Section: Discussionsupporting

confidence: 90%

“…There are limited reports regarding the element content in the same area of dental enamel during consecutive experimental phases (before and after treatment under several preventive protocols) [18,25]. Such analyses would allow for the objective measurement of the chemical changes that occur.…”

Section: Discussionmentioning

confidence: 99%

“…The distance between the tip and the sample was 1 mm, which was ensured by using a sheet of stainless steel (23 mm × 5 mm × 0 5 mm) that was fixed to the top of the laser handpiece. At this tip-sample distance, the exit tip and the laser beam had the same diameter, which was confirmed by a laminated infrared sensor screen (Lumitek International Inc., Ijamsville, MD, USA) [18,25,27,28]. Each sample was irradiated only once for 20 sec.…”

Section: Er:yag Lasermentioning

confidence: 94%

“…The crown was fixed to a glass slide with thermoplasticized epoxy resin (Allied, Rancho Dominguez, CA). Afterwards, a diamond wheel (South Bay Technology Inc.) was employed to obtain samples under constant irrigation [18,25,27,28].…”

Section: Tooth Selection and Sample Preparationmentioning

confidence: 99%

“…The samples were then cleaned for 5 min in separate containers filled with deionized water in an ultrasonic bath (Quantrex Q140, L&R Ultrasonics, NJ, USA) and dried at room temperature [25]. Specimens were scanned with a laser-fluorescence caries-detection system, the DIAGNOdent pen (KaVo, Biderach, Germany), and 50 specimens showing values between 0 and 13 (healthy teeth) were selected for the study and assigned to each group having no samples coming from the same tooth for each group.…”

Section: Tooth Selection and Sample Preparationmentioning

confidence: 99%

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Chemical Changes of Enamel Produced by Sodium Fluoride, Hydroxyapatite, Er:YAG Laser, and Combined Treatments

Ceballos-Jiménez

Rodríguez‐Vilchis

Contreras‐Bulnes

et al. 2018

Journal of Spectroscopy

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Occlusal pits and fissures of permanent molars are considered to have higher risk of developing caries. Enamel demineralization can be prevented by applying remineralizing agents, and their absorption increases with prior irradiation. This work evaluates the chemical changes produced by treating occlusal surfaces with sodium fluoride (NaF), hydroxyapatite-NaF-xylitol (HA-NaF-X), Er:YAG laser irradiation (L), and combinations thereof. Fifty enamel samples were randomly assigned to five groups (n = 10): NaF, HA-NaF-X, L, L + NaF, and L + HA-NaF-X. The chemical composition of human enamel was evaluated before (BT) and after (AT) treatment using energy-dispersive X-ray spectroscopy (EDS) and expressed in atomic percentages (at%). For combined treatment groups, the products were applied after laser irradiation. The statistical analyses included a paired t-test and ANOVA (p ≤ 0 05). After treatment, a significant increase in F at% was observed in the NaF group (2.71 ± 1.41). The irradiated groups showed significant increases in Ca and P at% and the Ca/P ratio. The highest values occurred for L + NaF (30.44 ± 4.28 Ca at%, 11.97 ± 1.45 P at%, and 2.55 ± 0.22 Ca/P ratio). Er:YAG laser irradiation alone or in combined protocols increased the Ca and P content of dental enamel, in vitro.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Er:yag Lasermentioning

confidence: 94%

Section: Tooth Selection and Sample Preparationmentioning

confidence: 99%

Section: Tooth Selection and Sample Preparationmentioning

confidence: 99%

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Chemical Changes of Enamel Produced by Sodium Fluoride, Hydroxyapatite, Er:YAG Laser, and Combined Treatments

Ceballos-Jiménez

Rodríguez‐Vilchis

Contreras‐Bulnes

et al. 2018

Journal of Spectroscopy

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Morphological and chemical changes in human deciduous dentin after phosphoric acid, self‐etching adhesive and Er: YAG laser conditioning

Moyaho-Bernal

Contreras‐Bulnes

Rodríguez‐Vilchis

et al. 2018

Microscopy Res & Technique

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The morphological and chemical changes in deciduous dentin produced by different conditioning protocols were evaluated in this in vitro study. Eighty primary dentin samples were divided into eight groups (n = 10): G1, acid etching; G2, self-etching adhesive; G3, G4, Er: YAG laser irradiation at 25.5 and 38.2 J cm , respectively; 10 Hz and spray irrigation. Groups 5, 6, 7, and 8 were irradiated at previous densities, and then phosphoric acid or self-etching adhesive conditioning was applied. Scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS) were used to evaluate chemical and morphological changes. Paired t-test and One-way ANOVA were used for statistical analysis (p ≤ 0.05). All samples showed different morphology with specific characteristics according to the conditioning protocol. Changing element concentration values are expressed in atomic percent (at %). After conditioning, there were statistically significant differences (p ≤ 0.05) for p at% and Ca/P in all groups; highlighting the following additional findings by group: G1, G7, and G8 showed changes in all elements studied, G2 presented a decrease in C at% and increased Ca at%, G3 and G4 exhibited at% changes in C, trace elements and Ca. Furthermore, G5 showed at% changes in O and trace elements; while G6 changes were observed on C at%, O at% and trace elements at%. Dentin morphology and chemical composition varied in accordance with the conditioning protocol, with characteristics specific for each one that could have clinical implications for the retention and bond strength performance of adhesive materials.

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Effect of Er:YAG laser irradiation on deciduous enamel roughness and bacterial adhesion: An in vitro study

et al. 2019

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Laser irradiation has been proposed as a preventive method against dental caries since it is capable to inhibit enamel demineralization by reducing carbonate and modifying organic matter, yet it can produce significant morphological changes. The purpose of this study was to evaluate the influence of Er:YAG laser irradiation on superficial roughness of deciduous dental enamel and bacterial adhesion. Fifty‐four samples of deciduous enamel were divided into three groups (n = 18 each). G1_control (nonirradiated); G2_100 (7.5 J/cm2) and G3_100 (12.7 J/cm2) were irradiated with Er:YAG laser at 7.5 and 12.7 J/cm2, respectively, under water irrigation. Surface roughness was measured before and after irradiation using a profilometer. Afterwards, six samples per group were used to measure bacterial growth by XTT cell viability assay. Adhered bacteria were observed using confocal laser scanning microscopy (CLSM) and a scanning electron microscopy (SEM). Paired t‐, one‐way analysis of variance (ANOVA), Kruskal‐Wallis and pairwise Mann–Whitney U tests were performed to analyze statistical differences (p < .05). Before treatment, samples showed homogenous surface roughness, and after Er:YAG laser irradiation, the surfaces showed a significant increase in roughness values (p < .05). G3_100 (12.7 J/cm2) showed the highest amount of Streptococcus mutans adhered (p < .05). The increase in the roughness of the tooth enamel surfaces was proportional to the energy density used; the increase in surface roughness caused by laser irradiation did not augment the adhesion of Streptococcus sanguinis; only the use of the energy density of 12.7 J/cm2 favored significantly the adhesion of S. mutans.

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Morphological and Chemical Changes of Deciduous Enamel Produced by Er:YAG Laser, Fluoride, and Combined Treatment

Cited by 21 publications

References 29 publications

Chemical Changes of Enamel Produced by Sodium Fluoride, Hydroxyapatite, Er:YAG Laser, and Combined Treatments

Chemical Changes of Enamel Produced by Sodium Fluoride, Hydroxyapatite, Er:YAG Laser, and Combined Treatments

Morphological and chemical changes in human deciduous dentin after phosphoric acid, self‐etching adhesive and Er: YAG laser conditioning

Effect of Er:YAG laser irradiation on deciduous enamel roughness and bacterial adhesion: An in vitro study

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