Morphological and biochemical diversity of Spiraea hypericifolia (Rosaceae) growing under natural conditions in Novosibirsk Oblast

Abstract

A study on some morphological characteristics and the set and levels of phenolic compounds was performed for the first time on Spiraea hypericifolia L. (Rosaceae Juss.) growing under natural conditions in the Novosibirsk region (four sites). Two phenotypic varieties of S. hypericifolia were identified. Twenty-three phenolic compounds were found in aqueous-ethanol extracts from S. hypericifolia leaves by high-performance liquid chromatography. Chlorogenic, p-hydroxybenzoic, and p-coumaric acids and quercetin, hyperoside, isoquercitrin, avicularin, rutin, and astragalin were identified among them. Hyperoside (6.7–20.5 mg/g) is the main phenolic compound in S. hypericifolia leaves. Flavonoid aglycones were prepared from the leaves by hydrochloric-acid hydrolysis of the aqueous-ethanol extracts (1:1). Three flavonol aglycones (quercetin, kaempferol, and isorhamnetin) were identified in the extract hydrolysates from S. hypericifolia leaves by chromatographic analysis. Quercetin glycosides proved to be predominant phenolic compounds in all the extract hydrolysates. A comparative analysis was performed on the levels of phenolic compounds in leaves among S. hypericifolia from the different sites and between the two varieties of S. hypericifolia. It was revealed that an increase in plant size by approximately twofold correlates with a decrease in concentrations of the identified flavonoids and phenolcarboxylic acids in S. hypericifolia leaves.

LC–HRMS for the Identification of Quercetin and Its Derivatives in Spiraea hypericifolia (Rosaceae) and Anatomical Features of Its Leaves

“…A literature review of the chemical composition of Spiraea species also showed that the majority (over 25) of flavonoid compounds are quercetin and its derivatives [ 25 ]. During an investigation of the chemical composition of some Spiraea species, we attempted to study phenolic components of Spiraea representatives from Asian Russia, including Spiraea hypericifolia L. [ 26 , 27 ]. Data on other chemical components of S. hypericifolia L. are fragmentary; the plant has been found to contain such compounds as flavonols, proanthocyanidins, and catechins [ 28 ].…”

Non-Targeted Screening of Metabolites in Aqueous-Ethanol Extract from Spiraea hypericifolia (Rosaceae) Using LC-HRMS

“…The analysis of metabolites in the retention time interval from 1 to 9 min helped us to identify compounds that are amino acids and sugars, as well as several phenolic compounds. Among the amino acids, the presence of L-lysine (1), L-tyrosine (2), proline (3), asparagine (5), and L-glutamic acid (4) was noted. From the class of sugars (retention time 1.65 min), α-lactose was identified (6).…”

“…Species of the genus Spiraea, including S. hypericifolia, are ornamental plants and are often used to decorate gardens and parks [1]. Species of the genus Spiraea are known as valuable ornamental plants, and for some of these species, the biological activity of their extracts and individual compounds has been documented, including anti-inflammatory, antioxidant, antiviral, antibacterial, antifungal, anticancer, and other effects [4][5][6][7][8][9][10]. Spiraea hypericifolia is one of these species.…”

“…The approaches involving metabolomics are based on an analysis of the qualitative and quantitative profiles of all extractable compounds as well as their correlations. This method is suitable for the purposes of pharmacology, where plant extracts as a Species of the genus Spiraea are known as valuable ornamental plants, and for some of these species, the biological activity of their extracts and individual compounds has been documented, including anti-inflammatory, antioxidant, antiviral, antibacterial, antifungal, anticancer, and other effects [4][5][6][7][8][9][10]. Spiraea hypericifolia is one of these species.…”