Morphological and Biochemical Blood Parameters in Broilers at Correction With Dietary Copper Salts and Nanoparticles

Sizova, Еlena; Korolev, V. L.; Макаев, Ш А; Miroshnikova, Elena P; Shakhov, Vladimir

doi:10.15389/agrobiology.2016.6.903eng

Cited by 7 publications

(2 citation statements)

References 6 publications

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“…An alternative to traditional forms of micronutrients can be nanopreparations containing metals (iron, cobult and copper) in the form of nanosized particles. The Scientific and Educational Center "Nano-and Biotechnologies" at Ryazan State Agrotechnological University, Russia, is studying the biological activity of metal nanopowders in various agricultural sectors (Polischuk et al, 2015a;Polischuk et al, 2018;Churilov et al, 2019), including the feeding of highly productive animals with the ability to catalyze biochemical processes in the body and increase metabolism (Nazarova et al, 2014;Miroshnikova et al, 2015;Sizova et al, 2016). With this background in mind, The aim of current study was to avaluate the effect of copper nanopowder on the mineral metabolism indicators during growth process of Holstein heifers.…”

Section: Introductionmentioning

confidence: 99%

Peculiarities of Mineral Metabolism of Holstein Heifers’ Diet Supplemented with Copper Nanopowders

Степанова¹,

Назарова²,

Arisov³

2020

WVJ

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The current study aimed to investigate the effect of copper nanopowder on physiological and mineral metabolism indicators of Holstein cattle during the growth process of the animal. There were two experimental groups (control and treatment) and each one included seven Holstein heifers. From the first month of each heifer in the experimental group, a suspension of copper nanoparticles in a dose of 0.04 mg/kg was orally administered daily to the usual diet. The biologically active additive was administered to the animal’s diet at intervals. The weight of the animals was measured monthly, a clinical blood test was performed, the mineral composition of the blood was studied, the mineral composition of animal hair was also examined. The findings indicated a positive dynamics in the increase of body weight in the treatment group, compared to the control group. As a result of clinical blood tests, it was noted that the number of erythrocytes, the level of hemoglobin, and hematocrit increased in the experimental animals due to copper nanoparticles compared to these parameters in these animals at the beginning of the tests. Mineral analysis of blood samples in the treatment group presented an increase in the levels of copper, potassium, iron, zinc, and manganese while in animals of the control groups there was an increase in sodium, calcium, and phosphorus. According to the blood serum and hair analysis, copper in the nanodispersed state indicated antagonistic effects on boron, silicon, antimony, molybdenum of the treatment group and there was a synergist in aluminum, titanium, manganese, cobalt, iron, and potassium levels compared to the beginning of the experiment. The obtained results indicated that the addition of copper nanopowder to the diet of experimental animals increased the growth, stimulated the function of hematopoiesis, and improved the characteristics of mineral metabolism of the Holstein heifers.

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Section: Introductionmentioning

confidence: 99%

Peculiarities of Mineral Metabolism of Holstein Heifers’ Diet Supplemented with Copper Nanopowders

Степанова¹,

Назарова²,

Arisov³

2020

WVJ

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“…It is expected to use a wide range of NPs for the creation of feed additives. The prospects of using NPs as sources of microelements [9], OVO additives [10], optimizers of the microbioma of animals, enhancers of immunity [11] and reproduction [12], growth promoters [13,14], for correction of weight gain composition and improvement of feed efficiency [15,16] have been shown.…”

Section: Introductionmentioning

confidence: 99%

Forecast of Productive and Biological Effects of Metal Nanoparticles According to Tolerance Index

Sizova¹

2019

GEOMATE

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In the experiment, different-sized NPs of the same elements are compared: iron nanoparticles sized 50.0±15 nm (FeaNPs) and iron nanoparticles sized 80.0±5 nm (FebNPs); copper nanoparticles sized 55.0±15 nm (CuaNPs) and copper nanoparticles sized 103.0±2 nm (CubNPs). Studies have shown that metal NPs with different physicochemical characteristics show different activity against E. coli K12 TG1 and have different productive effects on broiler chickens. The experimentally established constants of NP concentrations that determine the "quenching" of bioluminescence are closely related to the presence of biological action of metals on the chicken model. A new criterion for predicting the productive effect of NPs is proposed -the tolerance index (TI). The balance of the toxic dose of metal-microelement (EC50 for E. coli) and the biological level of microelement consumption determines the biological meaning of TI for broiler chickens (mmol/kg W 0.75 ×24 hours). As follows from calculations for metal NPs having TI<1, the absence of a productive effect is characteristic, for metal NPs having TI>1, on the contrary, the growth-stimulating effect on the model of broiler chicken is characteristic. Signs of the presence of oxidative stress and cell cytolysis, revealed by the activity of alanine aminotransferases (ALT), aspartate aminotransferases (ACT), lactate dehydrogenase (LDG), gammaglutamyl transferase (GGT), catalase (CT), total superoxide dismutase (T-SOD), malonic dialdehyde (MDA) were registered with the introduction of small-sized copper and iron nanoparticles with TI <1.

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Assessment of biotoxicity of Cu nanoparticles with respect to probiotic strains of microorganisms and representatives of the normal flora of the intestine of broiler chickens

Sizentsov

Кван

Miroshnikova

et al. 2018

Environ Sci Pollut Res

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Copper nanoparticle Cu (d = 55 ± 15 nm) and CuO nanoparticles (d = 90 ± 10 nm) were used in the studies (OOO Platina, Russia). Using the method of pure cultures, we extracted Lactobacillus, Enterococcus, and Enterobacterium from the intestines of broilers. Additionally, strains of Bacillus subtilis 10641 and Bifidobacterium were involved in probiotic strains. The data obtained in the course of the study testify to the insignificant biotoxicity of copper nanoparticles with respect to representatives of the genera Lactobacillus (30 to 15 μg/ml) and Bifidobacterium (30 μg/ml), with the most sensitive bacteria being the genus Lactobacillus, for which a concentration of 7.5 μg/ml was subinhibitory. The second stage was the study using method of agar wells. In the course of the experiment, we obtained results confirming the data of the research by the serial dilution method. In this case, as in the first case, the data indicate the insignificant biotoxicity of copper nanoparticles in relation to representatives of the genera Lactobacillus and Bifidobacterium. We have studied the bioaccumulating ability of microorganisms of the studied metals. In all the studies carried out, as in the first series of experiments, representatives of the genera Lactobacillus and Bifidobacterium with the lowest bioaccumulative ability were the most sensitive to copper nanoparticles and were 3.1 and 8.2%, respectively. The use of nanoparticles as a component of the fodder additive in small concentrations does not adversely affect not only the probiotic strains, but also the main representatives of the normoflora (Lactobacillus, Enterococcus, and Enterobacterium) of the poultry, the positive effect of the copper nanoparticles being directly related to low level of dissociation of nanoparticles, since biologically active ions will be released much more slowly, thereby creating a prolonged effect of exposure.

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Morphological and Biochemical Blood Parameters in Broilers at Correction With Dietary Copper Salts and Nanoparticles

Cited by 7 publications

References 6 publications

Peculiarities of Mineral Metabolism of Holstein Heifers’ Diet Supplemented with Copper Nanopowders

Peculiarities of Mineral Metabolism of Holstein Heifers’ Diet Supplemented with Copper Nanopowders

Forecast of Productive and Biological Effects of Metal Nanoparticles According to Tolerance Index

Assessment of biotoxicity of Cu nanoparticles with respect to probiotic strains of microorganisms and representatives of the normal flora of the intestine of broiler chickens

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