Morphological and biochemical analyses of the salivary glands of the malaria vector, Anopheles darlingi

Moreira-Ferro, C K; Marinotti, Osvaldo; Bijovsky, A. Tania

doi:10.1054/tice.1999.0057

Cited by 47 publications

(30 citation statements)

References 23 publications

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“…A similar difference in lobe size of salivary glands has been observed mosquito females, which exhibit three lobes of different sizes (43,44). Moreover, the results show a decrease in gland size between these two species of Lutzomyia post blood feeding in comparison with sugar solution feeding.…”

Section: Resultssupporting

confidence: 79%

Las glándulas salivales de dos flebotominos vectores de Leishmania: Lutzomyia migonei (França) y Lutzomyia ovallesi (Ortiz) (Diptera: Psychodidae)

et al. 2010

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Introduction. Leishmaniasis is a vector-borne disease transmitted by the intradermal inoculation of Leishmania (Kinetoplastida: Trypanosomatidae) promastigotes together with saliva during the bite of an infected sand fly. Objective. The salivary glands were compared from two vector species, Lutzomyia ovallesi (Ortiz,1952) and Lutzomyia migonei (França,1920) (Diptera: Psychodidae). Material and methods. Protein profiles by SDS PAGE of salivary glands were compared among species as well as their development at several times post feeding. First, mice were immunized to salivary proteins by exposure to biting by L. ovallesi and of L. migonei. Antibodies in these mice against salivary gland-specific proteins were evaluated by immunoblotting. Results. No apparent change was revealed in the kinetic expression of salivary proteins induced by the different physiological states post feeding. Qualitative and quantitative variations were detected in16-18 polypeptides with molecular weights ranging from 6 to 180 kDa. Species-specific proteins were demonstrated for L. migonei and L. ovallesi. In addition, antibodies against salivary gland specific proteins were found in mice immunized by the saliva of both species. Conclusion. Basic information was obtained concerning the nature of salivary gland proteins of L. migonei and L. ovallesi. This information helps to elucidate the role of salivary proteins and their potential as effective tools in screening risk factors in human and other vertebrate hosts.

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Section: Resultssupporting

confidence: 79%

Las glándulas salivales de dos flebotominos vectores de Leishmania: Lutzomyia migonei (França) y Lutzomyia ovallesi (Ortiz) (Diptera: Psychodidae)

et al. 2010

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“…While the female glands are organized in two identical lateral lobes and a medial lobe, the salivary glands of males consist of a single small lobe. The female gland is similar to the salivary glands of Aedes, Culex and Anopheles species 17,23,29,53 . Previous studies reported that the salivary glands of male Aedes and Culex mosquitoes are composed of three identical lobes, all of them formed by cells morphologically similar to that of the female proximal portion of the lateral lobes 43 .…”

Section: Discussionmentioning

confidence: 94%

“…albopictus 21 , An. darlingi 23 and Cx. quinquefasciatus 25 , demonstrated accumulation preferentially in the distal region and proximal region of the lateral lobes, respectively.…”

Section: Discussionmentioning

confidence: 99%

Salivary gland proteins of the human malaria vector, Anopheles dirus B (Diptera: Culicidae)

Jariyapan

Choochote

Jitpakdi

et al. 2007

Rev. Inst. Med. trop. S. Paulo

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SUMMARYSalivary gland proteins of the human malaria vector, Anopheles dirus B were determined and analyzed. The amount of salivary gland proteins in mosquitoes aged between 3 -10 days was approximately 1.08 ± 0.04 µg/female and 0.1 ± 0.05 µg/male. The salivary glands of both sexes displayed the same morphological organization as that of other anopheline mosquitoes. In females, apyrase accumulated in the distal regions, whereas alpha-glucosidase was found in the proximal region of the lateral lobes. This differential distribution of the analyzed enzymes reflects specialization of different regions for sugar and blood feeding. SDS-PAGE analysis revealed that at least seven major proteins were found in the female salivary glands, of which each morphological region contained different major proteins. Similar electrophoretic protein profiles were detected comparing unfed and blood-fed mosquitoes, suggesting that there is no specific protein induced by blood. Two-dimensional polyacrylamide gel analysis showed the most abundant salivary gland protein, with a molecular mass of approximately 35 kilodaltons and an isoelectric point of approximately 4.0. These results provide basic information that would lead to further study on the role of salivary proteins of An. dirus B in disease transmission and hematophagy.

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“…Male SG are much smaller and do not have, or have much smaller amounts of, activities associated with the blood meal such as antiplatelet, anticlotting, and vasodilatory. On the other hand, both sexes have similar amounts of activities associated with sugar meals such as glycosidases and antimicrobial agents that might prevent microbial growth in the mosquito crop, where sugar meals are stored before being diverted to the midgut for further digestion and absorption (Marinotti et al, 1990;Moreira-Ferro et al, 1999;Ribeiro et al, 1984;Rossignol and Lueders, 1986). Exceptionally, the mosquito genus Toxorhynchites comprises species that do not take any blood meal in the adult stage.…”

Section: Introductionmentioning

confidence: 99%

An insight into the sialotranscriptome of the non-blood feeding Toxorhynchites amboinensis mosquito

Calvo

Pham

Ribeiro

2008

Insect Biochemistry and Molecular Biology

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All adult mosquitoes take sugar meals, and most adult females also take blood meals to develop eggs. Salivary glands (SG) of males are thus much smaller and do not contain many of the antihemostatic and antiinflammatory compounds found in females. In the past 5 years, transcriptome analyses have identified nearly 70 different genes expressed in adult female SG. For most of these, no function can be assigned in either blood or sugar feeding. Exceptionally, Toxorhynchites mosquitoes are unusual in that they never feed on blood, and the SG of adults are identical in both sexes. Transcriptome analysis of the adult SG of this mosquito was performed to increase knowledge of the evolution of blood feeding-and to identify polypeptide families associated with sugar feeding-in mosquitoes.

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Morphological and biochemical analyses of the salivary glands of the malaria vector, Anopheles darlingi

Cited by 47 publications

References 23 publications

Las glándulas salivales de dos flebotominos vectores de Leishmania: Lutzomyia migonei (França) y Lutzomyia ovallesi (Ortiz) (Diptera: Psychodidae)

Las glándulas salivales de dos flebotominos vectores de Leishmania: Lutzomyia migonei (França) y Lutzomyia ovallesi (Ortiz) (Diptera: Psychodidae)

Salivary gland proteins of the human malaria vector, Anopheles dirus B (Diptera: Culicidae)

An insight into the sialotranscriptome of the non-blood feeding Toxorhynchites amboinensis mosquito

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