Morphological and anatomical aspects of birdsfoot trefoil and big trefoil

Abstract

-This study was carried out with the objective of describing the aerial and underground organs of birdsfoot trefoil [Lotus corniculatus (São Gabriel and ARS-2620 cultivars)] and big trefoil [Lotus uliginosus (Maku cultivar)]. The plants were cultivated in pots and harvested at 150 and 210 days after sowing. Stems, leaves and roots were prepared in according to conventional anatomical techniques. The anatomical structures of L. corniculatus and L. uliginosus differed in terms of pith cell integrity, starch content in the parenchyma root cell and underground stem cortex. The leaves were amphistomatic, with phenolic idioblasts, small vascular bundles and few conducting elements. The underground system was diffuse, with adventitious shoots that grew in two directions, had no cataphylls, emerged from the ground, and gave rise to aerial stems.The morphological characteristics of the shoot system of both species matched the recent descriptions of soboles. Key Words: Lotus corniculatus, Lotus uliginosus, rhizome, soboleAspectos morfológicos e anatômicos de cornichão-comum e cornichão-dos-banhados RESUMO -Este trabalho foi realizado para comparar e descrever anatomicamente os órgãos aéreos e subterrâneos de cornichão-comum [Lotus corniculatus (cv. São Gabriel; cv. ARS-2620)] e cornichão-dos-banhados [Lotus uliginosus (cv.Maku)]. As plantas foram cultivadas em vasos e colhidas aos 150 e 210 dias após semeadura. Amostras de caules, folhas e raízes foram preparadas de acordo com as técnicas convencionais de anatomia. As estruturas anatômicas das espécies L. corniculatus e L. uliginosus diferem quanto à integridade das células da medula das hastes, à quantidade aparente de amido nas células do parênquima das raízes e quanto ao tamanho da região cortical dos caules subterrâneos. As folhas são anfiestomáticas, possuem idioblastos fenólicos e feixes condutores de pequeno tamanho e com poucos elementos de condução. O sistema subterrâneo é difuso. Os caules têm origem adventícia, crescimento bipolar, não apresentam catáfilos e, ao emergirem do solo, originam ramos aéreos. As características morfológicas do sistema caulinar subterrâneo das duas espécies estão de acordo com a descrição recente aplicada ao sóbole.

Structure, histochemistry and phytochemical profile of the bark of the sobol and aerial stem of Tontelea micrantha (Celastraceae - Hippocrateoideae)

“…The underground stem of this plant was described as a xylopodium by Wanderley et al (2003), although xylopodium are tuberized structures derived from the hypocotyl and primary root. The morphology and anatomy of the underground stem of T. micrantha, as described here, suggests that its classifi cation as being a sobol, to be more correct -a diffuse stem-like underground system growing horizontally below the soil surface (Appezzato-da- Glória 2003, Maroso et al 2009). …”

Lignification and Tannin Localization During the Development of Birdsfoot Trefoil Stems

“…Both Lees et al (1993) and Brillouet and Escoute (2012) reported that condensed tannins were transparent and colorless in unstained plant material. Lees et al (1993) found similar localization of tannins alongside phloem fibers in the sainfoin leaf rachis, and Maroso et al (2009) found similar tannin localization in BFT stems harvested after 210 d of growth. The tannins found in BFT may be colored rather than transparent, considering the known variation in the chemical structure of tannins.…”

“…The tannins found in BFT may be colored rather than transparent, considering the known variation in the chemical structure of tannins. Lees et al (1993) found similar localization of tannins alongside phloem fibers in the sainfoin leaf rachis, and Maroso et al (2009) found similar tannin localization in BFT stems harvested after 210 d of growth. Staining with FeSO 4 and TBO indicated that the tannin content of BFT stems is minimal and does not increase with accumulation of stem dry matter as plants mature.…”

Biometric and Production Characterization of Some Lotus Corniculatus L. Genotypes

“…It is characterized by high adaptation, withstands low-fertility, acidic and arid soils (Maroso, 2009;Drobná, 2010;Ayres, 2007) but also with a potential for soil remediation, being often recommended as a cover species due to the fact that it fixes nitrogen in the soil (Yerlikaya, 2019). Following certain studies, its use for medicinal purposes its anti-spasmic and sedative role has been identified (Yerlikaya, 2019).…”