Morphological analysis of peripheral nerve regenerated by means of vein grafts filled with fresh skeletal muscle

Geuna, Stefano; Tos, Pierluigi; Battiston, Bruno; Guglielmone, R; Giacobini-Robecchi, Maria G.

doi:10.1007/s004290050334

Cited by 43 publications

(37 citation statements)

References 15 publications

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“…As regards biological nerve guides, various autologous tissues have proven to be effective in repairing sciatic nerve gaps with performances, in terms of both histological and functional predictors of recovery, that were close to those obtainable with autografts (Chiu et al, 1982;Glasby et al, 1986;Geuna et al, 2000).…”

Section: Tubulization Reconstruction Of Substance Defectsmentioning

confidence: 96%

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The sciatic nerve injury model in pre-clinical research

Geuna

2015

Journal of Neuroscience Methods

112

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In the pre-clinical view, the study of peripheral nerve repair and regeneration still needs to be carried out in animal models due to the structural complexity of this organ which can be only partly simulated in vitro. The far most used experimental model is based on the injury of the sciatic nerve, the largest nerve trunk in mammals. In this paper, the potential application of the sciatic nerve injury model in pre-clinical research is critically reviewed. This paper is aimed at helping researchers in properly employing this in vivo model for the study of nerve repair and regeneration as well as interpreting the results in a clinical translation perspective.

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Section: Tubulization Reconstruction Of Substance Defectsmentioning

confidence: 96%

“…Although the bridging of gaps longer than 1.5 cm have been described in the rat (e.g. Geuna et al, 2000;Dodla and Bellamkonda, 2008), it is preferable to move to large animal models (e.g. rabbit or sheep) when long nerve prostheses have to be tested in vivo.…”

Section: Surgerymentioning

confidence: 99%

The sciatic nerve injury model in pre-clinical research

Geuna

2015

Journal of Neuroscience Methods

112

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“…It was considered to be more accurate in an aspect that structural distortions of the nerve samples due to muscular contractions during the transcardial perfusion method could be avoided in the immersion protocol since the nerve samples, immediately after having been dissected, were immersed in a small drop of fixative solution, kept in a straight position for a few minutes followed by immersion in fixative solution for complete fixation 15,18 .…”

Section: Discussionmentioning

confidence: 99%

Comparison of Fixation Methods for Peripheral Nerve Fiber

Ulkay¹,

Aktaş²,

Aksoy³

et al. 2013

Kafkas Univ Vet Fak Derg

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Accurate fixation is a must for the assessment of myelinated nerve fiber morphology and transcardial perfusion and immersion methods are the most commonly used fixation techniques. In the present study we designed a new fixation technique for the histomorphometric and stereological evaluation of sciatic nerve fiber and referred it as instillation fixation. The method involved preliminary in situ fixation of the nerve sample without dissecting it from the animal body followed by a complete conventional fixation protocol. The objective of this study was to compare the three fixation techniques with each other for fixation artifacts. Eighteen female Wistar albino rats constituted the study material. The animals were allocated into three experimental groups corresponding to different fixation methods (immersion, instillation and transcardial perfusion, respectively). Quantitative assessments of nerve samples harvested from the animals of each group included the number of total myelinated axons, normal myelinated axons, alterations in myelin compaction, myelinated axons with irregular fiber shape, and with myelin loops and g ratio. Results revealed that normal myelinated axons were markedly lower in the immersion fixation group compared to those of instillation and transcardial perfusion. Moreover a significant decrease was noted with respect to alterations in myelin compaction in the instillation fixation group. In contrast, no significant difference was observed in myelin thickness and axon cross sectional area. In conclusion instillation fixation technique proved to be a valid and simple method for the assessment of peripheral nerve morphology for further analyses in a rat model. Keywords: Rat, Sciatic nerve, Fixation method Periferal Sinir Tespit Yöntemlerinin KarşılaştırılmasıÖzet Doğru bir tespit işlemi miyelinli periferik sinirlerin morfolojik değerlendirmesi için ön koşuldur. Kalp perfüzyonu ve daldırma en yaygın kullanılan tespit yöntemlerindendir. Çalışmamızda bu metotlara ek olarak damlatma tekniği olarak adlandırılan yeni bir tespit metodu uygulandı. Sinir doku örneği canlı hayvan üzerinden çıkarılmadan ön tespit işlemine tabi tutulduktan sonra rutin tespit işlemleri tamamlandı ve üç tespit yönteminin tespit artefaktları açısından birbirleriyle histomorfometrik ve stereolojik olarak karşılaştırılması gerçekleştirildi. On sekiz adet dişi Wistar albino sıçan kullanıldı. Hayvanlar sırasıyla daldırma, damlatma ve kalp perfüzyonu olmak üzere üç eşit gruba ayrıldı. Her bir gruptaki hayvanlara ait siyatik sinir örneklerinin kantitatif değerlendirilmesi toplam miyelinli akson sayısı, normal miyelinli akson sayısı, miyelin kompaksiyonundaki değişimler içeren aksonların sayısı, düzensiz demet yapısı ile kıvrımlı miyelin halkaları içeren miyelinli aksonların sayısı ve g oranları hesaplanarak gerçekleştirildi. Elde edilen bulgulara göre daldırma tespit grubundaki normal miyelinli akson sayısında, damlatma ve kalp perfüzyonu grupları ile karşılaştırıldığında belirgin bir düşüş belirlendi. Bununla beraber damlatma tesp...

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“…To prevent this occurrence, a luminal filler must be used for providing a substrate for early nerve fiber regeneration [5][6][7]. A number of different materials have been investigated as luminal fillers for nerve guides, including biological or artificial substrates [8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Peripheral Nerve Reconstruction Using Enriched Chitosan Conduits

Rochkind¹,

Mandelbaum-Livnat²,

StefaniaRaimondo³

et al. 2017

Scaffolds in Tissue Engineering - Materials, Technologies and Clinical Applications

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The repair of peripheral nerve traumatic lesions still represents a major cause of permanent motor and sensory impairment. In case of substance loss, a nerve guide should be used to bridge the proximal with the distal stump of the severed nerve. The effectiveness of hollow nerve guides is limited by the delay of axonal growth due to the absence of a regeneration substrate inside the conduit. To fasten up nerve regeneration, nerve guides should thus be enriched by a luminal filler. In this study, we investigated, in a 12-mm rat sciatic nerve defect experimental model, the effectiveness of chitosan-based conduits of different acetylation filled either with a hyaluronic acid gel (NVR gel) or with a magnetic fibrin hydrogel, in comparison with traditional autografts. Results showed that all types of artificial nerve conduits led to functional recovery not significantly different from autografts. By contrast, morphological and morphometrical analyses showed that the best results among nerve guides were found in medium degree of acetylation (DAII: ∼5%) chitosan conduits enriched with the NVR gel.

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Morphological analysis of peripheral nerve regenerated by means of vein grafts filled with fresh skeletal muscle

Cited by 43 publications

References 15 publications

The sciatic nerve injury model in pre-clinical research

The sciatic nerve injury model in pre-clinical research

Comparison of Fixation Methods for Peripheral Nerve Fiber

Peripheral Nerve Reconstruction Using Enriched Chitosan Conduits

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