Morphological analysis of embryonic cerebellar grafts in SCA2 mice

Purkartová, Zdeňka; Tůma, Jan; Pešta, Martin; Kulda, Vlastimil; Hájková, Lucie; Šebesta, Ondřej; Vožeh, F; Cendelín, Jan

doi:10.1016/j.neulet.2013.11.020

Cited by 12 publications

(3 citation statements)

References 26 publications

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“…Before staining, the sliced sections were washed in PB (2 × 10 min) and were blocked for 30 min at room temperature with agitation in 10% NDS and 1% Triton X‐100 (Sigma‐Aldrich) in PB. Secondary antibodies were diluted in antibody diluent at 1:400 NPY—donkey anti‐mouse/rabbit 488 (green) (Alexa Fluor® 488 AffiniPure donkey anti‐mouse) IgG (H + L) (Luo et al, 2015), and α‐MSH: donkey anti‐rabbit IgG (H + L), secondary antibody (red) (Alexa Fluor® 594 conjugate a21207) (Zhang, ), and c‐Fos donkey anti‐sheep 647 (purple) (Alexa Fluor® 647) (Purkartova et al, ). Sections were incubated with secondary antibodies (3 hr) at room temperature.…”

Section: Methodsmentioning

confidence: 99%

Caralluma fimbriata extract activity involves the 5‐HT2c receptor in PWS Snord116 deletion mouse model

2018

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IntroductionIn Prader–Willi syndrome (PWS), nonprotein coding small nucleolar (sno) RNAs are involved in the paternally deleted region of chromosome 15q11.2‐q13, which is believed to cause the hyperphagic phenotype of PWS. Central to this is SnoRNA116. The supplement Caralluma fimbriata extract (CFE) has been shown to decrease appetite behavior in some individuals with PWS. We therefore investigated the mechanism underpinning the effect of CFE on food intake in the Snord116del mouse. Experiments utilized appetite stimulants which included a 5‐hydroxytryptamine (5‐HT) 2c receptor antagonist (SB242084), as the 5‐HT2cR is implicated in central signaling of satiety.MethodsAfter 9‐week chronic CFE treatment (33 mg or 100 mg kg−1 day−1) or placebo, the 14‐week‐old Snord116del (SNO) and wild‐type mice (n = 72) were rotated through intraperitoneal injections of (a) isotonic saline; (b) 400 mg/kg of 2‐deoxyglucose (2DG) (glucose deprivation); (c) 100 mglkg beta‐mercaptoacetate (MA), fatty acid signaling; and (d) SB242084 (a selective 5HT2cR antagonist), with 5 days between reagents. Assessments of food intake were from baseline to 4 hr, followed by immunohistochemistry of neural activity utilizing c‐Fos, neuropeptide Y, and alpha‐melanocyte‐stimulating hormone within hypothalamic appetite pathways.Results Caralluma fimbriata extract administration decreased food intake more strongly in the SNO100CFE group with significantly stimulated food intake demonstrated during coadministration with SB242084. Though stimulatory deprivation was expected to stimulate food intake, 2DG and MA resulted in lower intake in the snord116del mice compared to the WT animals (p = <0.001). Immunohistochemical mapping of hypothalamic neural activity was consistent with the behavioral studies.ConclusionsThis study identifies a role for the 5‐HT2cR in CFE‐induced appetite suppression and significant stimulatory feeding disruptions in the snord116del mouse model.

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Section: Methodsmentioning

confidence: 99%

Caralluma fimbriata extract activity involves the 5‐HT2c receptor in PWS Snord116 deletion mouse model

2018

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“…The cerebellum represents a structure for which specific topographically arranged projections with other structures, but also connections within the cerebellar cortex as well as corticonuclear projections, are essential for its function [ 21 ]. A good source of cerebellar cell phenotypes to substitute lost cells is embryonic (fetal) cerebellar tissue [ 31 – 33 , 35 , 38 , 56 , 58 , 65 , 80 , 94 ]. Nevertheless, functional integration of this kind of graft is not easy (see above).…”

Section: Mechanisms Of the Effect Of The Graftsmentioning

confidence: 99%

Experimental neurotransplantation treatment for hereditary cerebellar ataxias

Cendelín

2016

cerebellum ataxias

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Hereditary cerebellar degenerations are a heterogeneous group of diseases often having a detrimental impact on patients’ quality of life. Unfortunately, no sufficiently effective causal therapy is available for human patients at present. There are several therapies that have been shown to affect the pathogenetic process and thereby to delay the progress of the disease in mouse models of cerebellar ataxias. The second experimental therapeutic approach for hereditary cerebellar ataxias is neurotransplantation. Grafted cells might provide an effect via delivery of a scarce neurotransmitter, substitution of lost cells if functionally integrated and rescue or trophic support of degenerating cells. The results of cerebellar transplantation research over the past 30 years are reviewed here and potential benefits and limitations of neurotransplantation therapy are discussed.

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“…Despite good graft survival and the presence of graft-derived Purkinje cells in a type-2 transgenic mice model of spinocerebellar ataxia, the structure of the graft did not show any significant specific functional effects. Nonetheless, further search for ways of enhancement of connections between the graft and the host is promising [51].…”

Section: Introductionmentioning

confidence: 99%

Cerebellar Nonmotor Functions – Approaches and Significance

Sveljo

Ćulić

2015

Neurophysiology

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The cerebellum is involved in the control of motor and nonmotor functions. Refined and innovative experimental and clinical approaches, starting from anatomy and including functional magnetic resonance imaging (fMRI), have allowed researchers to store extensive information on the cerebellar contributions to motor control and also helped them to understanding cerebellar nonmotor functions. Does the cerebellum process exclusively cerebral information related to certain specific actions, or does it also process some forms of information independent of such relation? At present, researchers are close to evaluating how the cerebellum is active during resolution of cognitive tasks. Various therapy lines in perspective, from cerebellar stimulation to cerbellar grafts and artificial cerebellum, are of particular significance, as they can restore lost brain functions in animal models and repair insufficient brain processes in patients.

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Morphological analysis of embryonic cerebellar grafts in SCA2 mice

Cited by 12 publications

References 26 publications

Caralluma fimbriata extract activity involves the 5‐HT2c receptor in PWS Snord116 deletion mouse model

Caralluma fimbriata extract activity involves the 5‐HT2c receptor in PWS Snord116 deletion mouse model

Experimental neurotransplantation treatment for hereditary cerebellar ataxias

Cerebellar Nonmotor Functions – Approaches and Significance

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