Morphological Analysis of Amoeboid–Mesenchymal Transition Plasticity After Low and High LET Radiation on Migrating and Invading Pancreatic Cancer Cells

Facoetti, Angelica; Gioia, Carmela Di; Pasi, Francesca; Liberto, Riccardo Di; Corbella, Franco; Nano, Rosanna; Ciocca, Mario; Valvo, Francesca; Orecchia, Roberto

doi:10.21873/anticanres.12763

Cited by 9 publications

(11 citation statements)

References 15 publications

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“…Seven studies ( 14 , 15 , 19 , 23 , 28 , 29 , 34 ) did not report whether the experiments were repeated, resulting in attrition bias. None of the publications stated whether the selection of treatment was blinded, and only one paper ( 21 ) reported blinding the outcomes assessors to minimize detection bias. All papers except one ( 28 ) described the implementation process.…”

Section: Resultsmentioning

confidence: 99%

“…The characteristics of included (13)(14)(15)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34) studies are summarized in Table 1. Briefly, 13 carbon ion irradiation and seven proton irradiation studies were included in our final SR; these studies were published between 2004 and 2021.They were conducted in 7 different countries: Japan, Germany, Belgium, Italy, Korea, the United States of America, and Lithuania.…”

Section: Study Characteristicsmentioning

confidence: 99%

“…There were five publications (13,(21)(22)(23)26) involved migration, invasion, or the expression of related genes (Table 5). Fujita and colleagues (13,22,23) conducted a series of studies and demonstrated carbon ion irradiation (2Gy) could repress the migration of AsPC, BxPC-3, and MIAPaCa-2 cells, diminish MIAPaCa-2 cell invasiveness via prevented the activity of Rac 1 and RhoA, and reduce the invasiveness of AsPC, BxPC-3 via inhibited the activity of Rac 1 through Ub-mediated proteasomal degradation.…”

Section: Migration and Invasion Abilitymentioning

confidence: 99%

“…Facoetti, A. 2018 (21) The migratory behavior of Aspc-1 cells is modulated by factors released by normal fibroblasts and tumor cells, and this is in turn modulated by both the radiation dose and the radiation quality. Fujita, M. 2014 (22) Nitric oxide increases the invasion of PANC-1 cells via activation of the PI3K-AKT and RhoA pathways after carbon ion irradiation.…”

Section: Author Year Migration and Invasion Abilitymentioning

confidence: 99%

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Charged Particle Irradiation for Pancreatic Cancer: A Systematic Review of In Vitro Studies

et al. 2022

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PurposeGiven the higher precision accompanied by optimized sparing of normal tissue, charged particle therapy was thought of as a promising treatment for pancreatic cancer. However, systematic preclinical studies were scarce. We aimed to investigate the radiobiological effects of charged particle irradiation on pancreatic cancer cell lines.MethodsA systematic literature search was performed in EMBASE (OVID), Medline (OVID), and Web of Science databases. Included studies were in vitro English publications that reported the radiobiological effects of charged particle irradiation on pancreatic cancer cells.ResultsThirteen carbon ion irradiation and seven proton irradiation in vitro studies were included finally. Relative biological effectiveness (RBE) values of carbon ion irradiation and proton irradiation in different human pancreatic cancer cell lines ranged from 1.29 to 4.5, and 0.6 to 2.1, respectively. The mean of the surviving fraction of 2 Gy (SF2) of carbon ion, proton, and photon irradiation was 0.18 ± 0.11, 0.48 ± 0.11, and 0.57 ± 0.13, respectively. Carbon ion irradiation induced more G2/M arrest and a longer-lasting expression of γH2AX than photon irradiation. Combination therapies enhanced the therapeutic effects of pancreatic cell lines with a mean standard enhancement ratio (SER) of 1.66 ± 0.63 for carbon ion irradiation, 1.55 ± 0.27 for proton irradiation, and 1.52 ± 0.30 for photon irradiation. Carbon ion irradiation was more effective in suppressing the migration and invasion than photon irradiation, except for the PANC-1 cells.ConclusionsCurrent in vitro evidence demonstrates that, compared with photon irradiation, carbon ion irradiation offers superior radiobiological effects in the treatment of pancreatic cancer. Mechanistically, high-LET irradiation may induce complex DNA damage and ultimately promote genomic instability and cell death. Both carbon ion irradiation and proton irradiation confer similar sensitization effects in comparison with photon irradiation when combined with chemotherapy or targeted therapy.

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Section: Resultsmentioning

confidence: 99%

Section: Study Characteristicsmentioning

confidence: 99%

Section: Migration and Invasion Abilitymentioning

confidence: 99%

Section: Author Year Migration and Invasion Abilitymentioning

confidence: 99%

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Charged Particle Irradiation for Pancreatic Cancer: A Systematic Review of In Vitro Studies

et al. 2022

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“…Cells were vertically irradiated in T12.5 flasks inside a water phantom put at the isocenter on the treatment table, at the depth of 15 cm, corresponding to the mid spread-out Bragg peak (SOBP). The SOBP (6 cm width, from 12 to 18 cm depth in water) was achieved with active beam energy modulation (9). Samples were irradiated at 0, 2 and 4 Gy.…”

Section: Methodsmentioning

confidence: 99%

Effects of L-DOPA Pretreatment on the Kinetics, Migration and Carbon Ion Radiation Response of T98G Cells

et al. 2018

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Background/Aim: Glioblastoma is the most malignant and widespread brain tumor in adults, with a rapid clinical course. Recently, it has been hypothesized that L-DOPA plays a role in the diagnosis and treatment of glioblastoma. The aim of this study was to assess the effects of pretreatment with L-DOPA on the biological behavior of human T98G cells in vitro. Materials and Methods: T98G cells were treated with 50 μg/ml or 100 μg/ml of L-DOPA for 4 h and their morphology, growth rate, clonogenic survival and migratory capacity in basal conditions and after carbon ion irradiation were evaluated using standard methods. Results: Treated cells showed a lower growth rate and an increased migratory capacity that correlated with the dose of tested L-DOPA. Treatment with L-DOPA increased the growth rate of carbon ion irradiated T98G cells compared to control non-treated cells exposed to the same radiation dose. Conclusion: Our results open further questions about the overall advantage of L-DOPA treatment of glioblastoma. Glioblastoma (GBM) is the most common and aggressive malignant brain tumor in adults, known for its aggressive proliferation and extensive invasion to normal tissue before any symptoms are presented, with long-term survival being extremely rare (1, 2).

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The clinical roadmap in a dual-beam hadrontherapy centre: tumour-based and patient-tailored selection criteria, management of range uncertainties and oncological patient pathway

Orlandi,

Lillo,

Camarda

et al. 2024

Health Technol.

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Purpose In the present short report, we encompass the radiobiological and dosimetric advantages of particle beam radiotherapy and we illustrate the oncological pathway for patients eligible for hadrontherapy being used at the National Center for Oncological Hadrontherapy (CNAO). Methods With a narrative approach, we examined the clinical and practical aspects of hadrontherapy compared to traditional photon beam radiotherapy. We described the need to select patients considering the intrinsic characteristics both of the tumour and the patient. Moreover, we described the decisional oncological tree and the patient pathway by our facility. Results Considering the dosimetric and radiobiological characteristics of particle beam radiotherapy, the selection of patients can not be done regardless of the intrinsic tumour and patient hallmarks. In particular, the tumour radioresistance, the patient radiosensitivity and the need to avoid post-actinic toxicities in long-term survivors should guide the clinical indication. The finite range of particles should be considered to avoid treatment uncertainties. Multidisciplinary national and international collaboration is crucial to better manage patients to treat with hadrontherapy and to create robust clinical evidence. Conclusion Hadrontherapy, with its distinctive physical and biological advantages, heralds a promising era in the field of precision radiotherapy. Patients’ radiosensitivity, tumour radioresistance, and treatment uncertainties should be considered to enhance the efficacy and to ameliorate the selection of patients.

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Morphological Analysis of Amoeboid–Mesenchymal Transition Plasticity After Low and High LET Radiation on Migrating and Invading Pancreatic Cancer Cells

Abstract: The migratory behavior of Aspc-1 cells is modulated by factors released by normal fibroblasts and tumor cells, and this is in turn modulated by both the radiation dose and the radiation quality.

Cited by 9 publications

References 15 publications

Charged Particle Irradiation for Pancreatic Cancer: A Systematic Review of In Vitro Studies

Charged Particle Irradiation for Pancreatic Cancer: A Systematic Review of In Vitro Studies

Effects of L-DOPA Pretreatment on the Kinetics, Migration and Carbon Ion Radiation Response of T98G Cells

The clinical roadmap in a dual-beam hadrontherapy centre: tumour-based and patient-tailored selection criteria, management of range uncertainties and oncological patient pathway

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