Morphological Alternations of Intraepithelial and Stromal Telocytes in Response to Salinity Challenges

Abstract

Summary statementThe article represent an experimental study in which we investigated the effect of the ssalinty on the communicating cells (telocytes) and their target cells including chloride, stem, Rodlet cells, myoblastsAbstractTelocyte is a communicating cell established relations to various types of cells. Few experimental studies are performed on telocytes. The current study investigated responce of telocytes to salinity stress in relations to osmoregualtory, immune and stem cells. We exposed Common carp to salinity level 0.2, 6, 10, 14 ppt. Gill samples were fixed and processed for microscopic and TEM. Two types of telocytes were identified: intraepithelial and stromal telocytes. Intraepithelial telocytes comprised the cellular lining of the lymph spaces where they shed the secretory vesicles. Stromal telocytes shed their secretory vesicles in the secondary circulatory vessels. Telocyte enlarged and exhibited high secretory activities. They exert their effect either by direct contact or by paracrine mode. In sanity treated samples, chloride cells enlarged and the mitochondria became cigar-shaped. pavement cells enlarged and micro-ridges elongated. Stromal telocytes established contact with stem cell and skeletal myoblast. Macrophages and Rodlet cells increased in number. In conclusion, intraepithelial and stromal responded to salinity stress by activation of cellular signaling. They play a major role in osmoregulation, immunity, and regeneration.

Recently discovered interstitial cells termed telocytes: distinguishing cell-biological and histological facts from fictions

“…To date, telocytes have been described in all classes of vertebrates, but also in many other animal species from different taxa. For instance, Soliman and Emeish studied the distribution of telocytes in the gills of carp species Cyprinus carpio in connection to the levels of water salinity (Soliman and Emeish 2017). Ghose et al (2008) discovered telocytes in the postcaval vein of the frog Rana tigrina and demonstrated that telocytes are responsible for the rhythmic contraction of this vein, independent of the electrical conduction system of the heart.…”

Detection of food fraud of meat products from the different brands by application of histological methods

“…The PhotoFiltre software (version 6.3.2; PhotoFiltre) was used to digitally color the SEM images to make it easier to identify the detailed structures of various tissues and organs on the same electron image (Abd‐Elhafeez & Soliman, 2016; Abd‐Elhafeez & Soliman, 2017; Abdel‐latief Soliman & Ali Emeish, 2017; Abdel‐Maksoud, Abd‐Elhafeez, & Soliman, 2019; Anwar, Abd‐Elhafeez, Abdel‐maksoud, & Abdalla, 2021; Soha Soliman, Hanan Hassan, & Enas, 2017; Soliman, 2021; Soliman & Abd‐Elhafeez, 2016; Soliman, Abd‐Elhafeez, Sa, & Hh, 2016; Soliman & Madkour, 2017; Soliman & Madkour, 2021) have used these techniques.…”

Telocytes are major constituents of the angiogenic apparatus

“…The samples underwent postfixation by osmium tetroxide, dehydration, impregnation in a mixture of alcohol/resin and pure resin, resin embedding, and crystallization in an oven at 60 °C. Semithin sections were taken at 1 μm using an ultramicrotome Ultracut E (Reichert-Leica, Germany) and stained with toluidine blue 20,21 and Periodic acid-Schiff (PAS) 22 . Staining of semithin sections was performed after dissolving the resin using a saturated alcoholic solution of sodium hydroxide.…”