Morphological alterations in the digestive diverticula of Mizuhopecten yessoensis (Bivalvia: Pectinidae) from polluted areas of Peter the Great Bay, Sea of Japan

Syasina, I. G.; Vaschenko, Marina A.; Жадан, П. М.

doi:10.1016/s0141-1136(96)00104-3

Cited by 25 publications

(13 citation statements)

References 32 publications

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“…In our study, regressive changes at digestive tissue level were frequently reported. The digestive gland tissue showed the most severe damage in the mussels from LD and PN over the samplings; the most represented lesions were lumen dilation, and tubule necrosis correlated with inflammatory lesions, as also previously reported in the bivalves from heavily stressful and polluted sites ( Syasina et al, 1997 ; Usheva et al, 2006 ).…”

Section: Discussionsupporting

confidence: 83%

Assessment of the Health Status of Mussels Mytilus galloprovincialis Along the Campania Coastal Areas: A Multidisciplinary Approach

et al. 2018

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The bivalve Mytilus galloprovincialis has a broad geographic distribution, represent an important species for the ecology of coastal waters, also constituting a major aquaculture species. In the present work, molecular and tissue biomarkers were examined in mussel populations (M. galloprovincialis) located in four different areas of the coastal water of the Campania Region. During an annual life cycle, we analyzed the expression patterns of several genes commonly used to estimate cellular stress response and damage, namely p53, p63, HSP70, MT-10, and MT-20, related tissue lesions (pathogens, inflammations, digestive tubules damage), oxidative stress indicators (H2O2, SOD specific activity) and associated environmental data. The computed Principal Component Analysis showed that the areas were discernible based on the environmental data and biomarker results. About animal health status, mussels from Gulf of Pozzuoli and Naples’s harbor did show a thinnest epithelial cell of digestive tubules compared to mussels sampled from other sampling sites; moreover, high prevalence of cases of intersex in three of the examinated areas were observed. The presence of a potential zoonotic pathogen (Nocardia crassostreae) was identified, appearing as an important possible emerging disease. We also reported the OIE notifiable protozoa Marteilia refringens in three areas out of four. The likely impact of both observed pathogens on the mussel health and shellfish aquaculture needs to be urgently addressed. Results are discussed considering animal histopathological health parameters and biological effects.

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Section: Discussionsupporting

confidence: 83%

Assessment of the Health Status of Mussels Mytilus galloprovincialis Along the Campania Coastal Areas: A Multidisciplinary Approach

et al. 2018

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“…Histopathological lesions in bivalves have already been related to soft-tissue concentrations of contaminants (Gold-Bouchot et al 1995). In general, the gills and digestive glands are, in mollusks, the major target organs for pollution studies (Gold-Bouchot et al 1995;Syasina et al 1997;Zaldibar et al 2007Zaldibar et al , 2008. Still, histopathology studies in C. edule are absent.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the potential of the common cockle (Cerastoderma edule L.) for the ecological risk assessment of estuarine sediments: bioaccumulation and biomarkers

et al. 2010

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Common cockles (Cerastoderma edule, L. 1758, Bivalvia: Cardiidae) were subjected to a laboratory assay with sediments collected from distinct sites of the Sado Estuary (Portugal). Cockles were obtained from a mariculture site of the Sado Estuary and exposed through 28-day, semi-static, assays to sediments collected from three sites of the estuary. Sediments from these sites revealed different physico-chemical properties and levels of metals and organic contaminants, ranging from unimpacted (the reference site) to moderately impacted, when compared to available sediment quality guidelines. Cockles were surveyed for bioaccumulation of trace elements (Ni, Cu, Zn, As, Cd and Pb) and organic contaminants (PAHs, PCBs and DDTs). Two sets of potential biomarkers were employed to assess toxicity: whole-body metallothionein (MT) induction and digestive gland histopathology. The bioaccumulation factor and the biota-to-soil accumulation factor were estimated as ecological indices of exposure to metals and organic compounds. From the results it is inferred that C. edule responds to sediment-bound contamination and might, therefore, be suitable for biomonitoring. The species was found capable to regulate and eliminate both types of contaminants. Still, the sediment contamination levels do not account for all the variation in bioaccumulation and MT levels, which may result from the moderate metal concentrations found in sediments, the species' intrinsic resistance to pollution and from yet unexplained xenobiotic interaction effects.

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“…Indeed, the digestive epithelium has been proposed to be fully regenerated every feeding cycle, which lasts a few hours (Nelson and Morton 1979). Regardless of whether such rapid cell renewal occurs, diverse sources of environmental stress have been demonstrated to cause a reorganisation of the epithelium resulting in an increased proportion of basophilic cells (Thompson et al 1974;Rasmussen et al 1983;Cajaraville et al 1990;Marigómez et al 1990Marigómez et al , 1998aSyasina et al 1997). This phenomenon, known as cell-type replacement (CTR), is clearly initiated after merely a few hours of environmental insult and constitutes a fully reversible process, with cell-type ratios returning to normal within a few days after the cessation of the stress (Marigómez et al 1998b).…”

Section: Introductionmentioning

confidence: 99%

“…In contrast, digestive cells proliferate after the cessation of exposure to environmental insults, as shown in recovery experiments under controlled laboratory conditions (Zaldibar et al 2001). Unfortunately, searches for unambiguous mitotic figures in the digestive gland have been unsuccessful (Thompson et al 1974;Rasmussen et al 1983;Cajaraville et al 1990;Marigómez et al 1990Marigómez et al , 1998aMarigómez et al , 1998bMarigómez et al , 1999Syasina et al 1997;Zaldibar et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Circatidal variation in epithelial cell proliferation in the mussel digestive gland and stomach

2004

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Epithelial cell renewal in mussel ( Mytilus galloprovincialis, Lmk) digestive gland and stomach was investigated by bromodeoxyuridine (BrdU) immunohistochemistry. Mussels were exposed to 4 mg BrdU/l seawater continuously. Starting at 6 h after treatment, samples were collected every 2 h for 2 days and BrdU labelling was estimated by direct counting at the light microscope, with values being noted per thousand BrdU-positive cells. BrdU-positive reaction was observed in the nuclei of digestive, basophilic, duct and stomach cells, and in haemocytes. Cell renewal in digestive diverticula was synchronised following a circatidal pattern: BrdU labelling increased during low tide and decreased during high tide. Clearcut mitotic figures were identified in digestive cells, thereby confirming that mature cell types proliferate, in agreement with results from immunohistochemistry for proliferating cell nuclear antigen and BrdU. Epithelial cell renewal in the stomach also appeared to be synchronised.

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Morphological alterations in the digestive diverticula of Mizuhopecten yessoensis (Bivalvia: Pectinidae) from polluted areas of Peter the Great Bay, Sea of Japan

Cited by 25 publications

References 32 publications

Assessment of the Health Status of Mussels Mytilus galloprovincialis Along the Campania Coastal Areas: A Multidisciplinary Approach

Assessment of the Health Status of Mussels Mytilus galloprovincialis Along the Campania Coastal Areas: A Multidisciplinary Approach

Evaluation of the potential of the common cockle (Cerastoderma edule L.) for the ecological risk assessment of estuarine sediments: bioaccumulation and biomarkers

Circatidal variation in epithelial cell proliferation in the mussel digestive gland and stomach

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