Morphologic, immunologic, enzymehistochemical and chromosomal analysis of a cell line derived from Hodgkin's disease. Evidence for a B-cell origin of sternberg-reed cells

Abstract

Cell lines derived from Hodgkin's disease may provide a clue to the nature of Sternberg‐Reed cells. In the current study, the establishment of an Epstein‐Barr‐virus‐negative lymphoblastoid cell line, derived from the pleural fluid of a patient with the nodular sclerosis type of Hodgkin's diseae, is described. The morphologic and immunologic cell marker findings indicate that this cell line is derived from Sternberg‐Reed cells. The immunologic findings and a chromosomal analysis are in agreement with a B‐lymphocyte origin of these cultured cells. Extrapolation of the results to Hodgkin's disease in vivo would indicate that Hodgkin's disease, like most non‐Hodgkin's lymphomas, is the result of B‐cell proliferation.

BCL6 alternative breakpoint region break and homozygous deletion of 17q24 in the nodular lymphocyte predominance type of Hodgkin's lymphoma–derived cell line DEV

“…Most of these cell lines are derived from nodular sclerosis or mixed cellularity-subtype CHL cases. The DEV cell line, although originally described as being derived from a nodular sclerosis-type CHL case [8], was retyped as of NLPHL origin [9] and is the only NLPHL-derived cell line.…”

“…Although the original histopathologic classification indicated a nodular sclerosissubtype CHL in 1985 [8], more recent subtyping clearly demonstrated that this HL case is, in fact, an NLPHL case [9]. The cell line is cultured in RPMI-1640 (Cambrex Biosciences, Walkersville, Md) supplemented with 20% fetal calf serum (Cambrex Biosciences), ultraglutamine-1 (Cambrex Biosciences), 100 IU of penicillin or 100 Ag/mL of streptomycin (Cambrex Biosciences) in 5% CO 2 at 378C.…”

Mutations in the genes coding for the NF- B regulating factors I B and A20 are uncommon in nodular lymphocyte-predominant Hodgkin's lymphoma

“…Initially, we wanted to confirm the NLPHL derivation of the DEV cell line, which was originally described to have been established from a cHL patient, 15 but which was later reclassified as being derived from NLPHL. 16 We used global gene expression profiles from primary microdissected HRS and LP cells, 4 and identified a set of 134 genes that best distinguished between them.…”

TP53 gene mutations in Hodgkin lymphoma are infrequent and not associated with absence of Epstein-Barr virus

“…The DEV cell line (EBV negative) was derived from nodular lymphocyte predominance HL. 24 Formalin-fixed, paraffin-embedded tissues from 67 Hodgkin cases were retrieved from the tissue bank of the Department of Pathology and Laboratory Medicine, University Hospital Groningen. The cases were sub-classified as nodular sclerosis (n ϭ 56), mixed cellularity (n ϭ 9), nodular lymphocyte predominance (n ϭ 1) and lymphocyte depleted (n ϭ 1).…”