Morphologic, cytometric and functional characterisation of Anadara kagoshimensis hemocytes

Кладченко, Е. С.; Andreyeva, A. Yu.; Кухарева, Т. А.; Солдатов, А. А.

doi:10.1016/j.fsi.2019.11.061

Cited by 17 publications

(4 citation statements)

References 12 publications

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“…Generally, granulocytes show higher phagocytosis capacities than hyalinocytes, which has been documented in oysters C. ariakensis (151) (113), M. unguiculatus (117), P. canaliculus (119), P. perna (120), P. viridis (122) and X. securis (124); and in freshwater mussels Amblema plicata, Quadrula quadrula (131), D. chilensis (128), L. marginalis (140) and S. cumingii (145) and in the pearl oyster P. imbricata (175). There are also studies where no differences in immune responses between the haemocytes types were found, as it happened in the ark clam A. kagoshimensis (86). In the clam Callista chione no differences in phagocytosis activity between granulocytes and hyalinocytes were observed (55).…”

Section: Phagocytosis and Encapsulationmentioning

confidence: 99%

Bivalve Haemocyte Subpopulations: A Review

et al. 2022

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Bivalve molluscs stand out for their ecological success and their key role in the functioning of aquatic ecosystems, while also constituting a very valuable commercial resource. Both ecological success and production of bivalves depend on their effective immune defence function, in which haemocytes play a central role acting as both the undertaker of the cellular immunity and supplier of the humoral immunity. Bivalves have different types of haemocytes, which perform different functions. Hence, identification of cell subpopulations and their functional characterisation in immune responses is essential to fully understand the immune system in bivalves. Nowadays, there is not a unified nomenclature that applies to all bivalves. Characterisation of bivalve haemocyte subpopulations is often combined with 1) other multiple parameter assays to determine differences between cell types in immune-related physiological activities, such as phagocytosis, oxidative stress and apoptosis; and 2) immune response to different stressors such as pathogens, temperature, acidification and pollution. This review summarises the major and most recent findings in classification and functional characterisation of the main haemocyte types of bivalve molluscs.

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Section: Phagocytosis and Encapsulationmentioning

confidence: 99%

Bivalve Haemocyte Subpopulations: A Review

et al. 2022

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“…La variedad celular confirma la complejidad funcional de este tejido y la posible participación en los mecanismos de tolerancia a las condiciones ambientales del manglar. Al igual que en la mayoría de los bivalvos, los granulocitos son un tipo celular abundante, los cuales contienen en su citoplasma numerosos gránulos ricos en diferentes moléculas de almacenaje, inmunitarias y pigmentos respiratorios (Allan & Raftos 2015, Kladchenko et al 2020a). Se ha sugerido que la aparición de varios tipos de gránulos intracelulares podrían estar relacionados con los procesos de diferenciación y maduración; concretamente los gránulos inmaduros son basófilos, y que al madurarse se convierten en acidófilos (Chen et al 1996).…”

Section: Discussionunclassified

Parámetros hemocitarios y química de la hemolinfa en reproductores de la concha prieta Anadara tuberculosa (Arcoida: Arcidae)

Espinoza

Zapata-Vívenes²,

Lodeiros³

2022

Rev peru biol

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Con motivo de conocer el estado de salud de poblaciones silvestres de la concha prieta Anadara tuberculosa se estudiaron los parámetros hemocitarios (viabilidad, conteo total y diferencial, estabilidad lisosomal, fragilidad osmótica) y la química sanguínea (hemoglobina-Hb, proteínas, triglicéridos, glucosa, lactato deshidrogenasa-LDH y catalasa-CAT). Se extrajo hemolinfa en reproductores salvajes aparentemente sanos colectados en la isla Corazón, río Chone, Ecuador. La viabilidad celular fue elevada, con presencia de 5 morfotipos: eritrocitos (74%), granulocitos traslucidos (6%), amebocitos (3%), hialinocitos (12%), blastocitos (5%). Los hemocitos presentaron membranas lisosomales estables al rojo neutro durante 240 min y una fragilidad osmótica media (FO50) de 4.8‰. Las concentraciones de Hb, proteínas, lípidos y glucosa denotan la función respiratoria y reservas energéticas durante los cambios de marea. La actividad de LDH está vinculada al metabolismo anaeróbico y CAT a la capacidad de mantener el equilibrio redox del sistema inmunitario. Los parámetros hemocitarios y química de la hemolinfa pueden servir como índices fisiológicos normales de referencia en reproductores de A. tuberculosa.

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“…A. kagoshimensis possesses salinity and temperature tolerance, and survives under hypoxia and anoxia. Hemocytes of three morphotypes circulating in hemolymph contain hemoglobin and, therefore, are characterized with high oxygen-carrying capacity in contrast to other benthic bivalves (De Zwaan et al, 2002, Xu et al, 2015Kladchenko et al, 2020). The unique functional, physiological and biochemical features found in soft tissues of blood clam reveal its great potential for research.…”

Section: Introductionmentioning

confidence: 99%

Respiration of invasive bivalve Anadara kagoshimensis (Tokunaga, 1906) at the 14-days starvation and different oxygen content in sea water

Vialova¹

2022

Preprint

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In the present work the influence of oxygen content in sea water and 14-days starvation on respiration rate of the bivalve species, Anadara kagoshimensis (Tokunaga, 1906), has been studied. The blood clams with shell height 25-30 mm have been used for experiments. Respiration rate varied in the wide range, from maximal value 89.9 mgO2·l-1 (at 100% saturation) to 5.0 µgО2·h-1·gww-1 (at 76% saturation) at the salinity 17-18 psu and temperature 18°C. The oxygen consumption of the blood clam was substantially lower compared to other benthic bivalves. Our results suggest the ability of A.kagoshimensis to rapidly transition from aerobic to anaerobic metabolism under conditions that are not critical for them. Two-weeks starvation led to an increase in respiration rates of the blood clam A. kagoshimensis, which indicates an intensification of oxidative processes of organism by 35 ˗ 55%. The article discusses metabolic processes that require increased oxygen consumption.

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Morphologic, cytometric and functional characterisation of Anadara kagoshimensis hemocytes

Cited by 17 publications

References 12 publications

Bivalve Haemocyte Subpopulations: A Review

Bivalve Haemocyte Subpopulations: A Review

Parámetros hemocitarios y química de la hemolinfa en reproductores de la concha prieta Anadara tuberculosa (Arcoida: Arcidae)

Respiration of invasive bivalve Anadara kagoshimensis (Tokunaga, 1906) at the 14-days starvation and different oxygen content in sea water

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