Morphologic characteristics of a transplantable tumor derived from a spontaneous malignant fibrous histiocytoma in the rat.

Yamate, Jyoji; Tajima, Masanori; Shibuya, Kazumoto; Ihara, Miheko; Kudo, Satoru

doi:10.1292/jvms1939.51.587

Cited by 16 publications

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“…Cultured MT-P cell line was induced from MFH-MT. The original and serially transplanted MFH-MT tumors, as well as tumors induced in syngeneic rats by injection with MT-P cells, were composed of an admixture of fibroblastic, histiocytic cells and immature mesenchymal cells arranged in a storiform growth pattern, of which histology was similar to that of human MFHs [ 96 , 98 ].…”

Section: Cellular Characteristics Of Mfhsmentioning

confidence: 99%

Stem cell pathology: histogenesis of malignant fibrous histiocytoma and characterization of myofibroblasts appearing in fibrotic lesions

Yamate¹

2023

The Journal of Veterinary Medical Science

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The concept of “stem cell pathology” is to establish the role of the stem cells by exploring their contribution to lesion development. The somatic stem cells are present in the body. Malignant fibrous histiocytoma (MFH; recently named “undifferentiated pleomorphic sarcoma”) includes pluripotential undifferentiated mesenchymal stem cells as a cell element. An antibody (A3) generated by using rat MFH cells as the antigen labels somatic stem cells such as bone marrow stem cells and immature endothelial cells and pericytes, as well as immature epithelial cells in epithelialization. By using A3 and other antibodies recognizing somatic stem cells, it is considered that myofibroblasts appearing in rat fibrotic lesions are developed partly from immature hepatic stellate cells in hepatic fibrosis, immature pancreatic stellate cells in pancreatic fibrosis, pericytes/endothelial cells in neovascularization in injured tissues, as well as via the epithelial-mesenchymal transition. These progenitors may be in the stem cell lineage. In this review, the author introduces the histogenesis of MFH and the characteristics of myofibroblasts appearing in fibrosis, based mainly on the author’s studies.

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Section: Cellular Characteristics Of Mfhsmentioning

confidence: 99%

Stem cell pathology: histogenesis of malignant fibrous histiocytoma and characterization of myofibroblasts appearing in fibrotic lesions

Yamate¹

2023

The Journal of Veterinary Medical Science

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“…A tissue fragment (2 mm in diameter) of MT-8 or MT-9 tumor was transplanted subcutaneously in the interscapular region of rats through a trochar of 2 mm in diameter under light anesthesia with ether. After the implantation, in order to obtain samples of the same size between MT-8 and MT-9 tumors, the major (a) and minor (b) axes of a developing tumor were measured in mm with a caliper, and the tumor volume was estimated by the formula a × b 2 /2 [34]. Out of eight rats in MT-8 implant group, two rats were sacrificed each on post-implantation (PI) days 3, 7, 9 and 17.…”

Section: Tumor Implantationmentioning

confidence: 99%

Expressions of Vascular Endothelial Growth Factor and Basic Fibroblast Growth Factor in Tumors Induced by Two Different Cloned Cell Lines Established from Transplantable Rat Malignant Fibrous Histiocytoma.

Tsunenari

Yamate

Sharma

et al. 2000

The Journal of Veterinary Medical Science

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ABSTRACT. In order to establish base-line data on angiogenic factors in development of mesenchymal tumors, expressions of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in implanted MT-8 and MT-9 tumors, both derived from a transplantable malignant fibrous histiocytoma (MFH) in the F344 rat, were investigated by immunohistochemistry and Western blotting method. MT-8 and MT-9 tumors were developed in syngeneic rats by implant of a tumor tissue fragment. MT-8 tumors were examined on post-implantation (PI) days 3, 6, 9 and 17, and MT-9 tumors were on PI days 9, 14, 17 and 23. The growth of MT-8 tumors was faster than that of MT-9 tumors. Histologically, MT-8 tumors were features of undifferentiated sarcomas, whereas MT-9 tumors exhibited a typical storiform growth pattern of MFH. Immunohistochemically, all cells constituting MT-8 and MT-9 tumors reacted with antibodies to VEGF and bFGF, indicating production of these factors by mesenchymal neoplastic cells. However, there were no marked differences in these immunoreactions between tumors examined. Thus, the bands obtained in the Western blotting methods were densitometrically scanned. The expression levels of VEGF and bFGF gradually increased PI day 3 to 9 in MT-8 tumors and PI day 9 to 17 in MT-9 tumors. On last examination day, the levels of bFGF in both tumors and of VEGF in MT-9 tumors decreased, but the VEGF expression level in MT-8 tumors was still increased. These findings indicated that VEGF and bFGF may contribute cooperatively to angiogenesis in an early growth of mesenchymal tumor development.

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“…Antibodies specific to certain cell types are very useful to pursue the kinetics and participation in genesis and lesion development of cells. To investigate the histogenesis of rat malignant fibrous histiocytoma (MFH), A3 was generated as a monoclonal antibody against MT-8 cells as the antigen 1 , 2 , 3 , 4 . MT-8 cells were established from a spontaneous rat MFH and regarded as primitive pluripotential mesenchymal cells that may be capable of differentiating into well-differentiated mesenchymal cells, such as adipogenic, osteogenic, and myofibrogenic cells 4 , 5 .…”

Section: Introductionmentioning

confidence: 99%

The localization and distribution of cells labeled by a somatic stem cell-recognizing antibody (A3) in rat colon development; possible presence of a new cell type forming the intestinal stem cell niche

et al. 2019

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A3, generated as a monoclonal antibody against rat malignant fibrous histiocytoma (MFH)-derived cloned cells, recognizes somatic stem cells (bone-marrow/hair follicle stem cells). We investigated the distribution of cells immunoreactive to A3 in the developing rat intestine (particularly, the colon), focusing on the ontogenic kinetics of A3-positive cells. In the rat intestine, A3 labeled spindle-shaped stromal cells localized in the submucosa and labeled endothelial cells of capillaries in the lamina propria forming villi in the early development stage. With development progression, A3-positive cells were exclusively localized around the crypts of the colon. Double immunofluorescence revealed that A3-positive cells around the crypts reacted to vimentin (for mesenchymal cells) and Thy-1 (for mesenchymal stromal cells) but not to α-SMA (for mesenchymal myofibroblastic cells) or CD34 (for hematopoietic stem cells), indicating that A3-positive cells around the crypts may have characteristics of immature mesenchymal cells. In addition, A3 labeled a few epithelial cells at the base of colon crypts. Furthermore, immunoelectron microscopy revealed that A3-positive cells lay inside myofibroblasts adjacent to the epithelium of the crypts. A3-positive cells were regarded as a new type of immature mesenchymal cells around the crypts. Collectively, A3-positive cells might take part in the stem cell niche in the colon, which is formed through epithelial-mesenchymal interaction.

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Morphologic characteristics of a transplantable tumor derived from a spontaneous malignant fibrous histiocytoma in the rat.

Cited by 16 publications

References 0 publications

Stem cell pathology: histogenesis of malignant fibrous histiocytoma and characterization of myofibroblasts appearing in fibrotic lesions

Stem cell pathology: histogenesis of malignant fibrous histiocytoma and characterization of myofibroblasts appearing in fibrotic lesions

Expressions of Vascular Endothelial Growth Factor and Basic Fibroblast Growth Factor in Tumors Induced by Two Different Cloned Cell Lines Established from Transplantable Rat Malignant Fibrous Histiocytoma.

The localization and distribution of cells labeled by a somatic stem cell-recognizing antibody (A3) in rat colon development; possible presence of a new cell type forming the intestinal stem cell niche

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