Morphologic and mucin histochemical analysis of transitional zones in advanced ulcerated colorectal carcinomas: potential prognostic indicators

Tamai, Osamu; Miyazato, Hiroshi; Shiraishi, Masayuki; Kusano, Toshiomi; Muto, Yoshihiro

doi:10.1002/(sici)1096-9098(199802)67:2<85::aid-jso3>3.0.co;2-h

Cited by 8 publications

(7 citation statements)

References 13 publications

(10 reference statements)

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“…The current study is one of the few which includes data from transitional tissue, and little is known on this subject so far. Some alterations in transitional tissue have been described regarding its healthy counterpart: sialomucins increase or hyperdevelopment and AG elongation, among others, suggesting that secretor activity is stimulated [27,28]. Despite the foregoing, it is unclear whether the neoplastic process leads to changes in the adjacent tumour mucosa or it is the other way around.…”

Section: Discussionmentioning

confidence: 99%

Synthesis and expression of CDw75 antigen in human colorectal cancer

et al. 2009

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BackgroundIncreased ST6Gal I activity has been associated with the α(2,6)sialylation enhancement of membrane glycoconjugates observed in metastatic colorectal carcinomas (CRC). Siaα(2,6)Galβ(1,4)GlcNAc sequence, known as CDw75, is a sialylated carbohydrate determinant generated by the ST6Gal I. This epitope has been reported to be associated with the progression of gastric and colorectal tumours, hence there are only a few conclusive studies to date.MethodsBy radioisotopic techniques we evaluated the ST6Gal I activity in healthy, transitional and tumour tissues from 43 patients with CRC. By immunohistochemistry we assessed the CDw75 expression in 25 colorectal adenomas, 43 tumours, 13 transitional and 28 healthy tissues of CRC patients.ResultsST6Gal I activity was likewise found to be statistically higher in tumour tissue respect to healthy tissue from CRC patients. CDw75 expression was positive in 20% of colorectal adenomas. Furthermore, 70% of tumour specimens and 8.3% of transitional specimens were positive for CDw75 expression, whereas none of the healthy ones showed the presence of the epitope.ConclusionThe major contribution of this study is the inclusion of data from transitional tissue and the analysis of CDw75 antigen expression in CRC and in colorectal adenomas, little known so far. ST6Gal I activity and CDw75 antigen expression were increased in CRC. Although their comparison did not reach the statistical significance, a great extent of patients showed both, an enhanced tumour ST6Gal I activity and an increased CDw75 expression in the tumour tissue. So, these two variables may play a role in malignant transformation. The expression of CDw75 in colorectal adenomas suggests that this antigen may be a tumour marker in CRC.

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Section: Discussionmentioning

confidence: 99%

Synthesis and expression of CDw75 antigen in human colorectal cancer

et al. 2009

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“…While it can be argued that the mucosa surrounding colorectal carcinomas is far from normal , there have been a number of observations of genetic lesions, especially in KRAS , in the apparently phenotypically‐normal mucosa from excision specimens . Similarly, the DNA repair gene O 6 ‐methylguanine‐DNA methyltransferase ( MGMT ) is frequently methylated in colorectal cancer, and fields of MGMT promoter methylation were seen in sporadic colorectal cancer as well as in the paired, apparently normal, colonic mucosa, showing near‐identical patterns of methylation associated with an increased incidence of KRAS mutations .…”

Section: Pre‐tumour Clones In Gastrointestinal Mucosamentioning

confidence: 99%

Pre‐tumour clones, periodic selection and clonal interference in the origin and progression of gastrointestinal cancer: potential for biomarker development

Baker

Graham²,

Wright

2013

The Journal of Pathology

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Classically, the risk of cancer progression in premalignant conditions of the gastrointestinal tract is assessed by examining the degree of histological dysplasia. However, there are many putative pro-cancer genetic changes that have occurred in histologically normal tissue well before the onset of dysplasia. Here we summarize the evidence for such pre-tumour clones and the existing technology that can be used to locate these clones and characterize them at the genetic level. We also discuss the mechanisms by which pre-tumour clones may spread through large areas of normal tissue, and highlight emerging theories on how multiple clones compete and interact within the gastrointestinal mucosa. It is important to gain an understanding of these processes, as it is envisaged that certain pre-tumour changes may be powerful predictive markers, with the potential to identify patients at high risk of developing cancer at a much earlier stage.

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“…Tumor stroma and contributes to the cancer initiation and progression [ 23 ]. We therefore investigated proliferation activity of stromal cells in the adenomatous/CRC stroma.…”

Section: Resultsmentioning

confidence: 99%

Temporal and spatial changes of cells positive for stem-like markers in different compartments and stages of human colorectal adenoma-carcinoma sequence

Cui

Zhu

et al. 2017

Oncotarget

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Considerable evidence supports the idea that stem-like cells may play an essential role during the development of colorectal cancer (CRC). To accomplish this aim, we use immunohistochemistry (IHC) and double IHC with different potential stem-like markers, anti-musashi (Msi), anti-CD133, anti- LGR5 and anti-ALDH1 to examine the presentation of stem-like cells in different compartments including adenoma/CRC epithelium, transitional crypts and tumor stroma in colorectal adenoma and CRC. The results showed that cells positive for stem-like markers were remarkably increased in number and frequently observed in the adenoma/CRC epithelium, transitional crypts and tumor stroma. Notably, the population of cells positive for stem-liker markers was expanded from the base to the middle part of the transitional crypt in both adenoma and CRC tissues, reflecting that stem-like cells are likely involved in the process of colorectal tumorigenesis. Counting results showed that the grading scores of cells positive for LGR5 and ALDH1 in the adenoma/CRC epithelium were significantly increased relative with the control epithelium, and associated with the degree of dysplasia in the adenoma and node involvement in the CRC (all P < 0.05). In addition, the density of cells positive for stem–like markers in the adenomatous/cancerous stroma was also increased and paralleled an increase in the density of proliferative stromal cells labeled by PCNA, which were primarily identified as vimentin positive fibroblasts. Our results have revealed a changed temporal and spatial presentation of stem-like markers in different stages of human colorectal adenoma-carcinoma sequence, which might be a hallmark of the adenoma-carcinoma transition.

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Morphologic and mucin histochemical analysis of transitional zones in advanced ulcerated colorectal carcinomas: potential prognostic indicators

Cited by 8 publications

References 13 publications

Synthesis and expression of CDw75 antigen in human colorectal cancer

Synthesis and expression of CDw75 antigen in human colorectal cancer

Pre‐tumour clones, periodic selection and clonal interference in the origin and progression of gastrointestinal cancer: potential for biomarker development

Temporal and spatial changes of cells positive for stem-like markers in different compartments and stages of human colorectal adenoma-carcinoma sequence

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