Morphologic and morphometric aspects of oenocytes of Apis mellifera queens and workers in different phases of life

Ruvolo, Maria Claudia Colla; Landim, Carminda da Cruz

doi:10.1590/s0074-02761993000300007

Cited by 13 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results, however, differ from those obtained in queens of Apis mellifera [15] , which have two distinct populations of oenocytes thus showing in basal ant species as well as derived ants here examined, an absence of functional compartmentalization among parietal and perivisceral cells.…”

Section: Morphology Histology and Histochemistrycontrasting

confidence: 99%

Fat Body Cells of Gynes and Queens of Four Species of Fungus Growing Ants (Hymenoptera: Formicidae: Attini); Relationship with the Vitellogenesis

Roma¹,

Camargo‐Mathias²,

Bueno³

2006

American J. of Agricultural and Biological Sciences

View full text Add to dashboard Cite

Abstract:The morphometric analysis of fat body cells of gynes of Cyphomyrmex rimosus and Mycetarotes parallelus and queens of Acromyrmex disciger and Atta laevigata revealed significant differences in area of parietal and perivisceral trophocytes in all studied species. This was also observed for parietal and perivisceral oenocytes. No differences, however, were observed when the areas of oenocytes of gynes are compared. The morphological study revealed that parietal and perivisceral trophocytes of gynes and queens were spherical or polygonal in shape and exhibited several vacuoles and a nucleus, often irregular in shape. These cells contained proteins, lipids and neutral polysaccharides. In gynes, however, histochemical tests for the former two components were weakly positive, as opposed to the observed in queens. Parietal and perivisceral oenocytes in all studied species were mostly spherical and exhibited vacuoles, especially in C. rimosus and A. laevigata, in which larger quantities of vacuoles were observed. The nuclei of oenocytes in gynes were spherical or oval in shape, while in queens, most were irregular. The cytoplasm of oenocytes was strongly stained for proteins and weakly stained for neutral polysaccharides in all studied species. Compared to queens, weak staining for lipids was observed in gynes.

show abstract

Section: Morphology Histology and Histochemistrycontrasting

confidence: 99%

Fat Body Cells of Gynes and Queens of Four Species of Fungus Growing Ants (Hymenoptera: Formicidae: Attini); Relationship with the Vitellogenesis

Roma¹,

Camargo‐Mathias²,

Bueno³

2006

American J. of Agricultural and Biological Sciences

View full text Add to dashboard Cite

show abstract

“…Roma et al 42 suggest that a few modifications had occurred during the evolution of the insects in the cells of the fat body, which resulted in the functional specialization of the individuals. Ruvolo and Cruz-Landim 43 confirmed that each of the castes has its own specificity in terms of cell size and place of occurrence in the body (e.g. head, thorax, etc.).…”

Section: Introductionmentioning

confidence: 86%

“…Therefore, the second aim was to answer the above question. The fat body has been characterized as the visceral part which surrounds the internal organs, and as the subcuticle element which, thanks to the loose arrangement of cells and being washed by the hemolymph, ensures metabolite exchange 28 , 43 , 47 , 48 . We applied a new approach to analyzing the subcuticular part of the fat body, not as a whole comprised between the 2nd and 7th abdominal segments, but as separate sets of cells in individual segments.…”

Section: Introductionmentioning

confidence: 99%

Segmentation of the subcuticular fat body in Apis mellifera females with different reproductive potentials

Strachecka

Olszewski

Kuszewska

et al. 2021

Sci Rep

View full text Add to dashboard Cite

Evolution has created different castes of females in eusocial haplodiploids. The difference between them lies in their functions and vulnerability but above all in their reproductive potentials. Honeybee queens are highly fertile. On the other hand, the workers are facultatively sterile. However, rebel workers, i.e. workers that develop in a queenless colony, reproduce more often than normal workers. As a result, the fat body of these bees, which apart from acting as the energy reserve, is also the site of numerous metabolic processes, had to specialize in different functions perfected over millions of years of eusocial evolution. Assuming that the variety of functions manifests itself in the pleomorphic structure of the fat body cells, we predicted that also different parts of the fat body, e.g. from different segments of the abdomen, contain different sets of cells. Such differences could be expected between queens, rebels and normal workers, i.e. females with dramatically different reproductive potentials. We confirmed all these expectations. Although all bees had the same types of cells, their proportion and segmental character corresponded with the caste reproductive potential and physiological characteristics shaped in the evolutionary process. The females with an increased reproductive potential were characterized by the presence of oenocytes in the third tergite and high concentrations of compounds responsible for energy reserves, like glucose, glycogen and triglycerides. Queens had very large trophocytes, especially in the third tergite. Only in workers did we observe intercellular spaces in all the segments of the fat body, as well as high protein concentrations—especially in the sternite. As expected, the rebels combined many features of the queens and normal workers, what with other findings can help understand the ways that led to the origin of different castes in females of eusocial Hymenoptera.

show abstract

“…The location of oenocytes varies in different insect species. In Drosophila , oenocytes form subepidermal clusters located on both sides of each abdominal segment 14 ; in Apis mellifera , oenocytes are present in groups below the epidermis in close association with the fat bodies 15 16 ; and in Tenebrio molitor , oenocytes lie among the fat bodies and are tightly associated with the tracheal system 17 .…”

mentioning

confidence: 99%

LmCYP4G102: An oenocyte-specific cytochrome P450 gene required for cuticular waterproofing in the migratory locust, Locusta migratoria

Zhang

Wang

et al. 2016

Sci Rep

View full text Add to dashboard Cite

Cytochrome P450 superfamily proteins play important roles in detoxification of xenobiotics and during physiological and developmental processes. To contribute to our understanding of this large gene family in insects, we have investigated the function of the cytochrome P450 gene LmCYP4G102 in the migratory locust Locusta migratoria. Suppression of LmCYP4G102 expression by RNA interference (RNAi) does not interfere with moulting but causes rapid loss of body weight - probably due to massive loss of water, and death soon after moulting. Accordingly, maintaining these animals at 90% relative humidity prevented lethality. Consistently, RNAi against LmCYP4G102 provoked a decrease in the content of cuticular alkanes, which as an important fraction of cuticular hydrocarbons have been shown to confer desiccation resistance. In addition, the cuticle of LmCYP4G102-knockdown locusts was fragile and easier deformable than in control animals. Presumably, this phenotype is due to decreased amounts of cuticular water that is reported to modulate cuticle mechanics. Interestingly, LmCYP4G102 was not expressed in the epidermis that produces the cuticle but in the sub-epdiermal hepatocyte-like oenocytes. Together, our results suggest that the oenocyte-specific LmCYP4G102 plays a critical role in the synthesis of cuticular hydrocarbons, which are important for cuticle waterproofing and mechanical stability in L. migratoria

show abstract

Morphologic and morphometric aspects of oenocytes of Apis mellifera queens and workers in different phases of life

Cited by 13 publications

References 0 publications

Fat Body Cells of Gynes and Queens of Four Species of Fungus Growing Ants (Hymenoptera: Formicidae: Attini); Relationship with the Vitellogenesis

Fat Body Cells of Gynes and Queens of Four Species of Fungus Growing Ants (Hymenoptera: Formicidae: Attini); Relationship with the Vitellogenesis

Segmentation of the subcuticular fat body in Apis mellifera females with different reproductive potentials

LmCYP4G102: An oenocyte-specific cytochrome P450 gene required for cuticular waterproofing in the migratory locust, Locusta migratoria

Contact Info

Product

Resources

About