Morphologic and Functional Characteristics of Alveolar Macrophages Following Cryopreservation

Lamb, Lawrence S.; Willoughby, Judith Barbaras; Willoughby, W. F.

doi:10.1006/cryo.1995.1035

Cited by 8 publications

(6 citation statements)

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“…The discrepancy with our results may be accounted for by our tissue processing protocol, which involved TA tissue cryopreservation at −80°C and sterilization by gamma irradiation before implantation. As reported by other researchers, 29 30 31 this treatment induces immunomodulation of the antigen response of biological structures without inducing morphological changes, 32 thus reducing tissue immunogenic power. 29 30 Finally, our grafts were sutured with 5-0 Vicryl degradable polymer suture, to reduce the risk of inflammation, 33 whereas Ferretti et al .…”

Section: Discussionmentioning

confidence: 70%

Microsurgical tunica albuginea transplantation in an animal model

Sansalone

Loreto

Leonardi³

et al. 2017

Asian J Androl

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Several andrological diseases require surgical repair or reconstruction of tunica albuginea, which envelops the corpora cavernosa penis. Despite intense research efforts involving a variety of biological materials, such as skin, muscle aponeurosis, human dura mater, tunica vaginalis, and pericardium, engineered tunica albuginea suitable for graft use is yet to be obtained. The study investigates microsurgical tunica albuginea allotransplantation in an animal model with the purpose of creation of an organ-specific tissue bank to store penile tissue, from cadaveric donors and male-to-female trans-sexual surgery, for allogeneic transplantation. Materials were tunica albuginea tissue explanted from 15 donor rats, cryopreserved at −80°C, gamma-irradiated, and implanted in 15 recipient rats, of which three rats were used as controls. Penile grafts were explanted at different time intervals; after macroscopic evaluation of the organ, the grafts were processed to morphological, histochemical, and immunohistochemical examinations by light microscopy. Detection of pro-inflammatory cytokines was also performed. Examination of the tunica albuginea allografts collected 1, 3, or 6 months after surgery and of control tunica albuginea fragments showed that tunica albuginea implants achieved biointegration with adjacent tissue at all-time points. The integration of cryopreserved rat tunica albuginea allografts, documented by our study, encourages the exploration of tunica albuginea allotransplantation in humans. In conclusion, the effectiveness and reliability of the tunica albuginea conditioning protocol described here suggest the feasibility of setting up a tunica albuginea bank as a further tissue bank.

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Section: Discussionmentioning

confidence: 70%

Microsurgical tunica albuginea transplantation in an animal model

Sansalone

Loreto

Leonardi³

et al. 2017

Asian J Androl

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“…In a previous study, we have shown that resident alveolar macrophages (AM) from individual rabbits can be frozen, stored, and recovered with morphology and selected functions intact retaining inducibte functions such as 02-release and production of tumor necrosis factor (TNF) and interleukin-1 (IL-1) (15). In this report, we have shown that normal resident AM may be pooled from outbred donor animals and frozen for up to 3 too.…”

Section: Letter To the Editor Cryopreserved Normal Macrophages As A Cmentioning

confidence: 96%

“…AM from individual donors and a pool of all three were processed and cryopreserved for up to 3 mo. as previously described (15). Each set of individual cells and the pool were tested before cryopreservation and following thawing for adherence as well as O2-, IL-1, and TNF release.…”

Section: Letter To the Editor Cryopreserved Normal Macrophages As A Cmentioning

confidence: 99%

Cryopreserved normal macrophages as a control for assays of macrophage function

Lamb

1999

In Vitro Cell.Dev.Biol.-Animal

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“…It seems that cryopreservation of bone marrow macrophage and mononuclear cells does not affect their morphology or their biological properties. 93 Their adherence is stable and they retain their ability to produce superoxide (O 2 ) anions, tumor necrosis factor (TNF) and interleukin-1 (IL-1).…”

Section: Cryopreservation Of Corneasmentioning

confidence: 99%